Peroxidase Activity in Native and Callus Culture of Moringa Oleifera Lam

Abstract

In vitro Moringa oleifera Lam. seedlings were successfully produced through seed culture on Murashige & Skoog (MS) agar medium containing 3% (w/v) sucrose and 0.2% (w/v) Gelrite TM in the absence of growth regulators under 1,500 lux of light density, 16 hour photoperiod light at temperature of 25 ± 2°C. Shoot-derived callus and rootderived callus of M. oleifera were established via culture of shoot and root on the MS medium supplemented with 0.5 mg/l of 2,4-dichlorophenoxy acetic acid (2,4-D) in the dark at 25 ± 2°C. Stem, leaf and root of native M. oleifera and M. oleifera callus were assayed for peroxidase activity using guaiacol as a substrate of the enzyme. In native plant, crude extract from root provided the highest peroxidase specific activity, followed by those from stem and leaf with the specific activity of 19.73 ± 0.18, 16.56 ± 1.43 and 13.38 ± 1.04 unit/mg protein, respectively. Crude extract of root-derived callus and shoot-derived callus of M. oleifera possessed specific activity of 167.25 ± 16.12 and 103.99 ± 10.64 unit/mg protein. These values are significantly higher than their counter parts from native M. oleifera suggesting the potential use of the callus cultures as new and improved sources of peroxidase.

Moringa oleifera in Vitro Culture and its Application as Anti-Diabetic in Alloxan Induced Diabetic Albino Mice

“…Callus, mainly comprising masses of undifferentiated cells, is good starting material for in vitro manipulation (Oriabi, 2013). But these results was disagreement with those obtained by Shank et al, (2013) who reported that MS medium supplemented with 0.5mg/l of 2,4-D was the most effective medium for callus induction of M. oleifera with 100% of callus induction from week-3 after culturing of shoot, Figure 1 showing the callus mass of M. oleifera which grown on MS medium after two subcultures. Results in table 3 described the changes in the weight of animals based on varying extract doses during the 15 days of treatment and showed that the body weight of mice significantly increased in the positive control group and those treated with 20, 40 and 80% of M. oleifera leaves or callus extracts compared with negative control which was significantly decreased with increasing days of treatment, these results was agreement with those obtained by Aja et al, (2013) who reported a significant (P<0.05) reductions in the mean body weight of rats in diabetic control compared to positive group while rats in treated groups showed significant (P<0.05) increase in their mean body weight compared to diabetic control group in work done earlier on antidiabetic effect of aqueous extract of Moringa oleifera and Bridelia ferruginea leaves in alloxan-induced diabetic albino rats.…”

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

“…They found that the activity of peroxidase on the callus of all explants was higher than that of the intact plant. Shank et al [54] examined the peroxidase activity in the calli of different explants of Moringa oleifera. They also showed that the enzyme activity was higher in callus than in plant.…”

Efficiently Removal of Copper and Cadmium from Wastewater using Activated Carbon Produced from Moringa oleifera Bark

“…Moringa oleifera have the valuable effect as medicine, nutrition, water management and so on [15]. Leaves, fruits, roots of MO are used as vegetables [23] and source of vitamins [24,25]. Other sides, various parts of MO such as stem, bark, and roots have antimicrobial activities [26,27].…”