Peroxidase activity of erythrocytes hemoglobin under action of low-frequency vibration

Abstract

Background. Hemoglobin is a hemoprotein which in the presence of oxidative equivalents, such as H2O2, can act as peroxidase with a very high oxidative potential. Hemoglobin oxidation is accompanied by generation of highly oxidized forms of iron and globin radicals that have high oxidative activity and are toxic to cells. In addition, peroxidase activity may indicate structural changes that occur in the hemoglobin molecule as a result of chemical modification. Materials and Methods. Erythrocyte suspension was subjected to vibration for 3 h within the frequency range from 8 to 32 Hz with amplitudes of 0.5 ± 0.04 and 0.9 ± 0.08 mm. At certain intervals, hemoglobin peroxidase activity was determined together with the content of its ligand forms in the hemolysates of cells. Additionally, experiments were performed to investigate the mechanism and calculate the kinetic parameters of peroxidase reaction. Results and Discussion. Experimental data on low-frequency vibrations effect on erythrocyte hemoglobin peroxidase activity were analyzed. The kinetics of the oxidation reaction of p-phenylenediamine by hemoglobin in erythrocytes was studied. It was found that peroxidase oxidation has a ping-pong mechanism. The kinetic parameters of the peroxidase reaction involving hemoglobin were determined. The change of kinetic parameters after two-hour exposure to the incubation medium and low-frequency vibration was studied. A possible mechanism of action of hemoglobin in oxidation reactions involving H2O2 was proposed. Conclusion. Any effect that initiates the formation of methemoglobin leads to an increase in the peroxidase activity of hemoglobin due to the involvement of the latter in the pseudoperoxidase cycle and the formation of toxic reactive globin radicals. The high content of oxyhemoglobin in the cell, observed under vibrations within the frequency range of 16–32 Hz with an amplitude of 0.9 ± 0.08 mm, can prevent its oxidation and involvement in the pseudoperoxidase cycle.

Mechanotransduction signaling pathways of erythrocytes associated with restructuring of cell metabolism

“…As we have shown earlier, vibration at a certain frequency, amplitude and duration leads to a decrease in the content of dissolved O 2 and CO 2 through degassing, creating a state of hypoxia (Dotsenko et al, 2021) and changing the intracellular pH. In this regard, all the signaling processes described above can be observed in cells under vibration conditions.…”

“…The hemoglobin content in the studied suspensions was at the level of 3.02 ± 0.18 mg/mL. Suspensions were exposed to low-frequency vibration for 3 hours in the frequency range from 8 to 32 Hz (Dotsenko & Troshchynskaya, 2014Dotsenko et al, 2021). The vibration frequency was changed in steps of 4 Hz.…”

“…The amplitude of vibration (A) was maintained at the level of 0.50 ± 0.04 and 0.90 ± 0.08 mm. Vibration was carried out with the help of a vibrostand, which consisted of a generator of low-frequency sinusoidal signals, an amplifier and a vibrator that oscillates in a vertical plane with a given frequency and amplitude (Dotsenko et al, 2021). The experimental cuvette, filled with a suspension of erythrocytes, was tightly fixed vertically on the moving part of the vibrator (in this case, mechanical vibrations are transmitted to the experimental cuvette with minor power losses).…”

“…Vibration can be perceived directly by membrane mechanoreceptors, as shaking creates fluid flows around the cell surface. Also, the target of vibration is nanobubbles of dissolved air, which are retained on the surface of erythrocytes due to Coulomb interactions and are able to coagulate and increase in size under the action of vibration (Bunkin et al, 2011;Dotsenko et al, 2021;Gudkov et al, 2021;Takahashi et al, 2021). It is assumed that the oxygen of the bubbles is involved in the processes of oxygenationdeoxygenation with the participation of hemoglobin (Bunkin et al, 2011).…”

Physical Fitness Training and Reaction Speed in Sports Table Tennis Players

“…In addition, the adjustment of the nervous system can enhance the coordination between the agonist, synergist, antagonist, and support muscles, thereby improving neuromuscular coordination, sensitivity, and precision. 14 In the test, some players ignored the accuracy of the movements to be fast. Some players are more careful.…”