Peroxidase activity of perennial ryegrass and tall fescue seedlings artificially infected with endophytes

Naffaa, Walid; Ravel, Catherine; Boyer, Nicole; Guillaumin, J. J.

doi:10.1051/agro:19990705

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…33 Except for the initial sampling period, peroxidase activity remained decreased in response to PM389. This result is supported by results of Naffaa et al 34 which suggested that the reduction in peroxidase level may be a possible mechanism to facilitate endophytic colonization of bacteria. On the contrary, peroxidase activity was not enhanced in response to pathogen up to 12 HAI indicating suppression of oxidative burst in the initial phase of infection.…”

Section: Discussionsupporting

confidence: 81%

Endophytic Bacteria Pseudomonas aeruginosa PM389 Subsists Host’s (Triticum aestivum) Immune Response for Gaining Entry Inside the Host

Gupta¹,

Das²,

Jha³

2021

J. Pure Appl. Microbiol.

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The present study was designed to compare the defense response of the host plant towards endophytic bacteria Pseudomonas aeruginosa PM389 and pathogenic bacteria Erwinia carotovora and to correlate the level of defense enzymes vis-a-vis bacterial colonization in the host. Wheat seedlings were treated with 107-108 cells ml-1 endophytic and pathogenic bacteria in the separate experimental set-up, and the level of plant defense enzyme was measured at various time intervals. Comparatively reduced level of most defense enzymes was produced in endophytic bacteria treated plants. While the endophytic bacterial population was almost constant after 24 HAI (hour after inoculation), the population of pathogenic bacteria kept fluctuating during the study period from 24 HAI. Unlike pathogenic bacteria, we observed attenuated defense response in challenged host plants towards endophytic bacteria, which helps endophytes establish inside plant. This study would be useful for understanding the mechanism of colonization and strategies of endophytes to fight against the host defense response.

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Section: Discussionsupporting

confidence: 81%

Endophytic Bacteria Pseudomonas aeruginosa PM389 Subsists Host’s (Triticum aestivum) Immune Response for Gaining Entry Inside the Host

Gupta¹,

Das²,

Jha³

2021

J. Pure Appl. Microbiol.

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“…Further proteomic studies done earlier documented significant suppression of peroxidase, Mn-superoxide dismutase, PR10 and stress-induced protein during nodule emergence in legume-rhizobium association [58]. Suppression of peroxidase (PR9) in the host during colonization is supported by other reports as well [59,60]. Like PR9, PR2 resulted in a similar trend supported by downregulation of β-glucanase (MtBGLU1) gene from the roots of M. truncatula after 24 h of inoculation of Sinorhizobium meliloti [61].…”

Section: Discussionsupporting

confidence: 61%

Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region

Gupta

Paul

Singh

et al. 2022

Land

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Diazotrophic endophytic bacteria (DEB) are the key drivers of nitrogen fixation in rainfed soil ecosystems and, hence, can influence the growth and yield of crop plants. Therefore, the present work investigated the structure and composition of the DEB community at different growth stages of field-grown pearl millet plants, employing the cultivation-dependent method. Diazotrophy of the bacterial isolates was confirmed by acetylene reduction assay and amplification of the nifH gene. ERIC-PCR-based DNA fingerprinting, followed by 16S rRNA gene analysis of isolates recovered at different time intervals, demonstrated the highest bacterial diversity during early (up to 28 DAS (Days after sowing)) and late (63 DAS onwards) stages, as compared to the vegetative growth stage (28–56 DAS). Among all species, Pseudomonas aeruginosa was the most dominant endophyte. Assuming modulation of the immune response as one of the tactics for successful colonization of P. aeruginosa PM389, we studied the expression of the profile of defense genes of wheat, used as a host plant, in response to P. aeruginosa inoculation. Most of the pathogenesis-related PR genes were induced initially (at 6 h after infection (HAI)), followed by their downregulation at 12 HAI. The trend of bacterial colonization was quantified by qPCR of 16S rRNAs. The results obtained in the present study indicated an attenuated defense response in host plants towards endophytic bacteria, which is an important feature that helps endophytes establish themselves inside the endosphere of roots.

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“…Gliocladium-like endophytes have been reported in perennial ryegrass and they can sporulate on the host plant [33]. Artificial infection of tall fescue and perennial ryegrass plants with Gliocladium-like endophytes resulted in a significant increase in peroxidase activity in leaf sheaths [34]. This indicates that Gliocladium-like endophytes can trigger a systemic defense mechanism, a response typical for infection with parasitic fungi.…”

Section: Introductionmentioning

confidence: 90%

Epichloë (formerly Neotyphodium) fungal endophytes increase adaptation of cool-season perennial grasses to environmental stresses

Malinowski¹,

Belesky

2019

Acta Agrobot

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Many cool-season grass species have evolved with asexual, nonsymptomatic fungal endophytes of the genus <em>Epichloë</em> (formerly <em>Neotyphodium</em>) of the family Clavicipitaceae. These associations range from parasitic to mutualistic and have dramatic effects on grass host chemistry, increasing resistance to abiotic (drought, soil mineral imbalance) and biotic (vertebrate and invertebrate herbivory, nematodes, plant pathogens, plant competition) stresses. Native endophyte strains produce a range of bioprotective alkaloid and other nonalkaloid secondary compounds, several of them known to have detrimental effects on grazing animals. In the past two decades, epichloid endophyte strains have been selected with marginal or no capacity of producing ergot and/or lolitrem alkaloids. These novel endophyte strains have been introduced to several grass cultivars with the idea to increase grass host resistance to abiotic stresses without hindering grazing livestock, and abiotic stresses to ensure high competitive ability of symbiotic grass cultivars. In this presentation, we discuss mechanisms underlying the competitiveness of epichloid endophyte/grass associations and consequences of endophyte infection for grassland ecosystem functions.

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Peroxidase activity of perennial ryegrass and tall fescue seedlings artificially infected with endophytes

Abstract: -An increase in peroxidase activity is a common response of plants to various stresses, especially to infection by pathogens. It

Cited by 7 publications

References 23 publications

Endophytic Bacteria Pseudomonas aeruginosa PM389 Subsists Host’s (Triticum aestivum) Immune Response for Gaining Entry Inside the Host

Endophytic Bacteria Pseudomonas aeruginosa PM389 Subsists Host’s (Triticum aestivum) Immune Response for Gaining Entry Inside the Host

Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region

Epichloë (formerly Neotyphodium) fungal endophytes increase adaptation of cool-season perennial grasses to environmental stresses

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