Peroxidase-Based Amperometric Sensor for the Determination of Total Phenols Using Two-Stage Peroxidase Reactions

Kong, Young Tae; Imabayashi, Shin Ichiro; Kano, Kenji; Ikeda, Tokuji; Kakiuchi, Takashi

doi:10.5344/ajev.2001.52.4.381

Cited by 26 publications

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“…14 The principal mainstay in such sensors is enzyme immobilization onto the working electrode. Tyrosinase, laccase, polyphenol oxidase and z E-mail: sudeshnadatta@yahoo.com; baishali.kanjilal@gmail.com; sarkarpriya@ gmail.com peroxidase have been immobilized onto a variety of substrates using physical adsorption, 19,20 entrapments into polymer matrices 21,22 and by glutaraldehyde crosslinking onto substrates with appropriate functionalities. 23 The choice of method of immobilization provides a wide range of studies to monitor efficiency of the sensor by efficient electron transfer mechanism.…”

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Electrochemical Sensor for Detection of Polyphenols in Tea and Wine with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy Utilizing Tyrosinase and Gold Nanoparticles Decorated Biomembrane

Datta

Kanjilal

Sarkar

2017

J. Electrochem. Soc.

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This paper reports a biocompatible sensor based on gold nanoparticles (AuNPs) and enzyme tyrosinase (Tyr) modified egg shell membrane (ESM) on glassy carbon electrode (GCE) as an effective analytical tool for the detection of polyphenols. AuNPs were synthesized on the membrane from gold chloride solution (HAuCl 4 ) without the application of toxic reducing agent. The sensor exhibited excellent linear relationship (r > 0.99) with micromolar concentrations of analyte and high sensitivity in differential pulse voltammetry for gallic acid (GA), caffeic acid (CA) and catechin hydrate (CH). The detection limits were found to be 1.707 μM, 0.752 μM and 0.714 μM for GA, CA and CH respectively (S/N = 3) at three different anodic potentials vs Ag/AgCl reference electrode (pH 6). Results showed good sensor reusability without loss of sensitivity. Modified membrane without AuNPs was also analyzed to detect polyphenol content by simple colorimetry. The modified sensor was characterized by electrochemical impedance spectroscopy (EIS). The developed sensor was successfully employed for detection of polyphenol content in commercially available tea and wine samples and results compared to traditional methods e.g. HPLC. The sensitivity values (193.9, 82 and 73.4 nA μM −1 cm −2 for CH, GA and CA) were high enough making the method a promising tool for practical gradation of real samples.

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Electrochemical Sensor for Detection of Polyphenols in Tea and Wine with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy Utilizing Tyrosinase and Gold Nanoparticles Decorated Biomembrane

Datta

Kanjilal

Sarkar

2017

J. Electrochem. Soc.

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An Amperometric Biosensor for trans‐Resveratrol Determination in Aqueous Solutions by Means of Carbon Paste Electrodes Modified with Peroxidase Basic Isoenzymes from Brassica Napus

et al. 2008

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The catalytic properties of peroxidase basic isoenzymes (PBIs) from Brassica napus towards trans-resveratrol (t-Res) oxidation were demonstrated by the first time by conventional UV-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBIs to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBIs entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBIs-Fc-CP) and is based on the fact that the decreased amount of H 2 O 2 produced by the action of PBIs is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBIs activity was much lower than commercial horseradish peroxidase (HRP) activity, t-Res was a much better substrate for PBIs biosensors than those biosensors constructed by using HRP. The PBIs-Fc-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (c t-Res ) range from 1 Â 10 À6 to 2.5 Â 10 À5 M, with a sensibility of (2.31 AE 0.05) Â 10 6 nA M À1. The lowest c t-Res value measured experimentally for a signal to noise ratio of 3 : 1 was 0.83 mM.

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State of the Art in the Field of Electronic and Bioelectronic Tongues – Towards the Analysis of Wines

et al. 2009

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This review compares various types of (bio)electronic tongues. The design and principles of potentiometric and voltammetric electronic tongues are discussed together with applications in food and environmental analysis. Different approaches towards bioelectronic tongue are presented. Several methods for evaluation and interpretation of the measured data are described. Finally, the potential of such devices for analysis of wine is discussed.

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Peroxidase-Based Amperometric Sensor for the Determination of Total Phenols Using Two-Stage Peroxidase Reactions

Cited by 26 publications

References 0 publications

Electrochemical Sensor for Detection of Polyphenols in Tea and Wine with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy Utilizing Tyrosinase and Gold Nanoparticles Decorated Biomembrane

Electrochemical Sensor for Detection of Polyphenols in Tea and Wine with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy Utilizing Tyrosinase and Gold Nanoparticles Decorated Biomembrane

An Amperometric Biosensor for trans‐Resveratrol Determination in Aqueous Solutions by Means of Carbon Paste Electrodes Modified with Peroxidase Basic Isoenzymes from Brassica Napus

State of the Art in the Field of Electronic and Bioelectronic Tongues – Towards the Analysis of Wines

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