2015
DOI: 10.1016/j.ajpath.2015.01.013
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Peroxidase Enzymes Regulate Collagen Extracellular Matrix Biosynthesis

Abstract: Myeloperoxidase and eosinophil peroxidase are heme-containing enzymes often physically associated with fibrotic tissue and cancer in various organs, without any direct involvement in promoting fibroblast recruitment and extracellular matrix (ECM) biosynthesis at these sites. We report herein novel findings that show peroxidase enzymes possess a well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functiona… Show more

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Cited by 35 publications
(37 citation statements)
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“…The area of tumour necrosis was then quantified as a percentage of total tumour area. For immunohistochemistry, sections (6 µm thick) of 4T1 tumours were probed with primary antibodies; rabbit anti-human collagen type I polyclonal; rabbit anti-human collagen type VI polyclonal (Rockland Immunochemicals, Inc., Pottstown, PA, UsA), mouse anti-human α-sMA (sigma-Aldrich, st. Louis, MO, UsA), rabbit anti-human CD31 (Abcam, Cambridge, Uk) as previously described (14). Quantitative analysis of the degree of positive staining was performed by digitized image analysis with ImageJ software as previously described (18).…”
Section: Animals Five Week-old Female Balb/c Mice (Institute Ofmentioning
confidence: 99%
See 3 more Smart Citations
“…The area of tumour necrosis was then quantified as a percentage of total tumour area. For immunohistochemistry, sections (6 µm thick) of 4T1 tumours were probed with primary antibodies; rabbit anti-human collagen type I polyclonal; rabbit anti-human collagen type VI polyclonal (Rockland Immunochemicals, Inc., Pottstown, PA, UsA), mouse anti-human α-sMA (sigma-Aldrich, st. Louis, MO, UsA), rabbit anti-human CD31 (Abcam, Cambridge, Uk) as previously described (14). Quantitative analysis of the degree of positive staining was performed by digitized image analysis with ImageJ software as previously described (18).…”
Section: Animals Five Week-old Female Balb/c Mice (Institute Ofmentioning
confidence: 99%
“…Cell viability/ growth was then assessed using the AlamarBlue™ fluorescent dye assay (Invitrogen Life Technologies, Melbourne, Australia) as per manufacturer's instructions. The amount of soluble type I and VI collagen in cell-conditioned medium was measured by a direct coat ELIsA method using standard curves constructed from purified type I and VI human placental collagen (BD Biosciences) as previously described (14).…”
Section: Animals Five Week-old Female Balb/c Mice (Institute Ofmentioning
confidence: 99%
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“…119 ROS toxicity is one of the first identified predictive toxicity paradigms of nanomaterials, which has been extensively studied in the last two decades. 118,128 However, unbalanced capability of intracellular ROS scavenging unavoidably leads to inflammatory reactions and DNA damage. OH˙); 121-124 3) NPs trigger mitochondria dysfunction, leading to an imbalance of the respiratory chain and disturbed ROS signalling; 125,126 4) macrophages and neutrophils produce ROS when activated by NPs.…”
Section: Oxidative Stress Inflammation and Genotoxicitymentioning
confidence: 99%