Peroxidase mimetic activity of fluorescent NS-carbon quantum dots and their application in colorimetric detection of H<sub>2</sub>O<sub>2</sub> and glutathione in human blood serum

Singh, Vikas Kumar; Yadav, Pradeep Kumar; Chandra, Subhash; Bano, Daraksha; Talat, Mahe; Hasan, S. H.

doi:10.1039/c8tb01286e

Cited by 98 publications

(39 citation statements)

References 69 publications

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“…Enzymes are good catalysts in many biological processes [16], but require specific physiological conditions to perform their optimal catalytic activity. The peroxidase enzyme catalyzes the oxidation of the substrate in the presence of hydrogen peroxide (H 2 O 2 ), acting as electron acceptor.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

et al. 2020

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Recent developments in the area of nanotechnology have focused on the development of nanomaterials with catalytic activities. The enzyme mimics, nanozymes, work efficiently in extreme pH and temperature conditions, and exhibit resistance to protease digestion, in contrast to enzymes. We developed an environment-friendly, cost-effective, and facile biological method for the synthesis of ZnO-Pd nanosheets. This is the first biosynthesis of ZnO-Pd nanosheets. The synthesized nanosheets were characterized by UV–visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray. The d-spacing (inter-atomic spacing) of the palladium nanoparticles in the ZnO sheets was found to be 0.22 nm, which corresponds to the (111) plane. The XRD pattern revealed that the 2θ values of 21.8°, 33.3°, 47.7°, and 56.2° corresponded with the crystal planes of (100), (002), (112), and (201), respectively. The nanosheets were validated to possess peroxidase mimetic activity, which oxidized the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate in the presence of H2O2. After 20 min of incubation time, the colorless TMB substrate oxidized into a dark-blue-colored one and a strong peak was observed at 650 nm. The initial velocities of Pd-ZnO-catalyzed TMB oxidation by H2O2 were analyzed by Michaelis–Menten and Lineweaver–Burk plots, resulting in 64 × 10−6 M, 8.72 × 10−9 Msec−1, and 8.72 × 10−4 sec−1 of KM, Vmax, and kcat, respectively.

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Section: Introductionmentioning

confidence: 99%

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

et al. 2020

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“…In the case of pristine Bi2O3, the peaks at 431, 509 and 525 cm −1 can be assigned to the vibrations of Bi-O bonds in α-Bi2O3 [44][45][46]. For the spectrum of the 15C/3R-Bi2O3-x sample, the characteristic peaks of Bi2O3 are also detected, and the deformation vibration of C-H (~635 cm −1 ), the stretching vibration of C-C (~1630 cm −1 ) and the stretching vibration of C-OH (~1120 cm −1 ) are found [47,48]. This demonstrates the presence of CQDs in the composite.…”

Section: Xrd and Ftir Analysismentioning

confidence: 92%

Carbon Quantum Dots (CQDs) Decorated Bi2O3-x Hybrid Photocatalysts with Promising NIR-Light-Driven Photodegradation Activity for AO7

Tang

Sun

et al. 2019

Catalysts

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In this work, Bi2O3-x with surface oxygen vacancies was prepared through the NaBH4 reduction of Bi2O3. After that, carbon quantum dots (CQDs) were deposited onto the surface of the Bi2O3-x to obtain a series of the CQDs/Bi2O3-x composites. The HRTEM and XPS characterizations of the CQDs/Bi2O3-x composites suggest that the thickness of surface oxygen vacancies could be adjusted by changing the concentration of NaBH4 solution, and the intimate contact between CQDs and the Bi2O3-x is achieved. Acid orange 7 (AO7) was adopted as the target reactant for investigating the photocatalytic degradation activities of the CQDs/Bi2O3-x composites under simulated sunlight and NIR light irradiation. It is found that the photocatalytic activities of the samples are closely related to the concentration of NaBH4 and content of CQDs. The Bi2O3-x samples exhibit enhanced simulated-sunlight-driven photocatalytic activity compared with Bi2O3. Specifically, the optimal degradation efficiency of AO7 is achieved over the 3R-Bi2O3-x (concentration of NaBH4: 3 mmol/L), which is 1.38 times higher than the degradation AO7 efficiency over Bi2O3. After the decoration of the 3R-Bi2O3-x surface with CQDs, the simulated-sunlight-driven photocatalytic activity of the CQDs/Bi2O3-x composite could be further enhanced. Among the samples, the 15C/3R-Bi2O3-x sample reveals the highest photocatalytic activity, leading to an AO7 degradation percentage of ~97% after 60 min irradiation. Different from Bi2O3 and the 3R-Bi2O3-x, the 15C/3R-Bi2O3-x sample also exhibits near-infrared (NIR)-light-driven photocatalytic degradation activity. In addition, the intrinsic photocatalytic activity of CQDs/Bi2O3-x composite was further confirmed by the degradation of phenol under simulated sunlight and NIR light irradiation. The photocurrent response and electrochemical impedance spectroscopy (EIS) measurements confirm the efficient migration and separation of photogenerated charges in the CQDs/Bi2O3-x samples. The •OH and h+ are proved to be the main reactive species in the simulated sunlight and NIR light photocatalytic processes over the CQDs/Bi2O3-x composites. According to the above experiments, the photocatalytic degradation mechanisms of the CQDs/Bi2O3-x composites under simulated sunlight and NIR light illumination were proposed.

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“…The biosynthesized nanoclusters exhibited ultrasensitive and selective detection of H 2 O 2 . The novelty of the reported method for the detection of H 2 O 2 , in comparison with previously reported methods, is presented in a tabulated form in Table 1 [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. The biosynthesis of palladium nanoclusters in itself is a novel achievement, and no reports are available on the use of biological methods for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species

Tripathi

Chung

2020

Molecules

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Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a simple, ultrasensitive, and selective method for H2O2 detection is crucial, this remains a strategic challenge. The peroxidase mimetic activity of palladium nanoclusters (PdNCs) has not previously been evaluated. In this study, we developed an ultrasensitive and selective colorimetric detection method for H2O2 using PdNCs. An unprecedented eco-friendly, cost-effective, and facile biological method was developed for the synthesis of PdNCs. This is the first report of the biosynthesis of PdNCs. The synthesized nanoclusters had a significantly narrow size distribution profile and high stability. The nanoclusters were demonstrated to possess a peroxidase mimetic activity that could oxidize peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Various interfering substances in serum (100 μM phenylalanine, cysteine, tryptophan, arginine, glucose, urea, Na+, Fe2+, PO43−, Mn+2, Ca2+, Mg2+, Zn2+, NH4+, and K+) were included to evaluate the selectivity of the assay, and oxidation of TMB occurred only in the presence of H2O2. Therefore, PdNCs show an efficient nanozyme for the peroxidase mimetic activity. The assay produced a sufficient signal at the ultralow concentration of 0.0625 µM H2O2. This colorimetric assay provides a real-time, rapid, and easy-to-use platform for the detection of H2O2 for clinical purposes.

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Peroxidase mimetic activity of fluorescent NS-carbon quantum dots and their application in colorimetric detection of H₂O₂ and glutathione in human blood serum

Abstract: Synthesis of nitrogen–sulphur dual-doped carbon quantum dots and their peroxidase like mimetic activity for the colorimetric detection of H2O2 and glutathione in human blood serum.

Cited by 98 publications

References 69 publications

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Carbon Quantum Dots (CQDs) Decorated Bi2O3-x Hybrid Photocatalysts with Promising NIR-Light-Driven Photodegradation Activity for AO7

Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species

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Peroxidase mimetic activity of fluorescent NS-carbon quantum dots and their application in colorimetric detection of H2O2 and glutathione in human blood serum

Abstract: Synthesis of nitrogen–sulphur dual-doped carbon quantum dots and their peroxidase like mimetic activity for the colorimetric detection of H2O2 and glutathione in human blood serum.

Cited by 98 publications

References 69 publications

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Enzyme Mimetic Activity of ZnO-Pd Nanosheets Synthesized via a Green Route

Carbon Quantum Dots (CQDs) Decorated Bi2O3-x Hybrid Photocatalysts with Promising NIR-Light-Driven Photodegradation Activity for AO7

Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species

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Peroxidase mimetic activity of fluorescent NS-carbon quantum dots and their application in colorimetric detection of H₂O₂ and glutathione in human blood serum