Peroxidase-producing actinobacteria from Algerian environments and insights from the genome sequence of peroxidase-producing Streptomyces sp. S19

Maibeche, Rima; Boucherba, Nawel; Bendjeddou, K.; Prins, Alaric; Bouiche, Cilia; Hamma, Samir; Benhoula, Mohammed; Azzouz, Zahra; Bettache, Azzeddine; Benallaoua, Saı̈d; Roes‐Hill, Marilize Le

doi:10.1007/s10123-022-00236-x

Cited by 5 publications

(2 citation statements)

References 86 publications

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“…Genomic DNA was isolated from SF actinobacterial isolates using a modified bead-beating method (Miller et al 1999) as described by Maibeche et al (2022). A colony PCR protocol was used for the ANP isolates.…”

Section: Isolation Of Genomic Dna and 16s Rrna Gene Sequence Analysismentioning

confidence: 99%

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Prins,

Dyani,

Vreulink

et al. 2024

Ann Microbiol

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Background South Africa is known for its great biodiversity. The Agulhas Plain represents one such unique environment where low-gradient topography has resulted in extensive wetland formation. It is fed by two major river systems, bringing in brackish, alkaline water. It has been exposed to major marine transgression and regression events, and harbours great Fynbos diversity as well as a Mediterranean-type climate, thereby creating unique ecosystems. It is therefore surprising that little is known about the bacterial diversity associated with the Agulhas Plain and associated marine ecosystems. Methods In this study, we focused on the actinobacterial diversity (Phylum Actinomycetota) associated with an emerging peatland on the Agulhas Plain (SF; Areas 1–3) and a marine site (ANP; Ocean, Rocky, Dry) located 10 km away from SF. A combined metataxanomics and isolation approach was taken to evaluate the actinobacterial diversity of the sampling sites and to determine the effect of environmental physicochemical parameters on these populations. Various genome analyses were performed on an Sva0096 marine bin to gain insight into its ecological role. Results Metataxanomics showed that the two sites shared defined major taxa, including Blastococcus, Geodermatophilus, Microbacterium, Mycobacterium, Nocardioides, Streptomyces, and the Sva0996 marine group. Analysis of the biosynthetic potential of an Sva0996 marine bin134 (obtained from GenBank) provided insights into the potential ecological role of this group of bacteria in both the marine and terrestrial environments. Higher actinobacterial diversity (Shannon index > 5) was observed for Areas 2 and 3 (SF), as well as the ANP Dry samples. The actinobacterial population composition was found to be driven by salinity, pH, Mn, and Ca, with certain areas of SF exhibiting similar (and even higher) salinity (SF: 70–100 Ω vs. ANP: 100–160 Ω) and lower pH levels (SF: 6.3-8.0 vs. ANP: 8.6–8.9) to that of the marine environment. Conclusion This snapshot study has provided some insights into the actinobacterial diversity of the two sites studied. Analysis of an Sva0096 marine bin134 provided further insights into the potential ability of the Sva0096 marine group to survive in a unique terrestrial environment that is periodically exposed to environmental pressures that mimic the marine environment.

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Section: Isolation Of Genomic Dna and 16s Rrna Gene Sequence Analysismentioning

confidence: 99%

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Prins,

Dyani,

Vreulink

et al. 2024

Ann Microbiol

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“…Furthermore, it was reported that the marine-derived S. rochei MHM13 and S. rochei MS-37 have the ability to synthesize silver nanoparticles, exhibiting a wide range of inhibition against both Gram-positive and -negative bacteria [13,14]. Additionally, enzymes with biotechnological interest [15,16] are produced by a new soil-derived Streptomyces rochei subsp. chromatogenes NEAE-K [17].…”

Section: Introductionmentioning

confidence: 99%

Statistical Medium Optimization for the Production of Anti-Methicillin-Resistant Staphylococcus aureus Metabolites from a Coal-Mining-Soil-Derived Streptomyces rochei CMB47

et al. 2023

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The development of novel antibacterial drugs needs urgent action due to the global emergence of antibiotic resistance. In this challenge, actinobacterial strains from arid ecosystems are proving to be promising sources of new bioactive metabolites. The identified Streptomyces rochei strain CMB47, isolated from coal mine Saharan soil, provided an ethyl acetate extract which tested against a series of pathogens. It displayed a minimum inhibitory concentration of <0.439 µg/mL against MRSA. A statistical experimental design using a response surface methodology (RSM) based on the second-order rotatable central composite design (RCCD) was planned to develop an efficient fermentation process able to improve the bioactive metabolite production. The optimal conditions were determined for starch and NaNO3 concentrations, incubation time and the initial pH value, reaching the inhibition zone diameter of 20 mm, close to the experimental value, after validation of the model. A bioassay-guided fractionation of the crude extract provided the most active fractions, which were analyzed by HPLC equipped with a photodiode array detector and coupled online with an electrospray mass spectrometer (HPLC-DAD/ESI-MS), obtaining preliminary indications on the molecular structures of the metabolites. These results support the potential interest in further investigations into the purification and full characterization of the metabolites responsible for the biological activity observed so far.

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Olive mill wastewater biodegradation for bacterial lipase production using a response surface methodology

Benhoula

Azzouz

Bettache

et al. 2023

Biomass Conv. Bioref.

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Peroxidase-producing actinobacteria from Algerian environments and insights from the genome sequence of peroxidase-producing Streptomyces sp. S19

Cited by 5 publications

References 86 publications

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Actinobacteria diversity associated with marine sediments and a wetland system, Agulhas-South Africa

Statistical Medium Optimization for the Production of Anti-Methicillin-Resistant Staphylococcus aureus Metabolites from a Coal-Mining-Soil-Derived Streptomyces rochei CMB47

Olive mill wastewater biodegradation for bacterial lipase production using a response surface methodology

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