Peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic approaches

Heawchaiyaphum, Chukkris; Pientong, Chamsai; Phusingha, Pensiri; Vatanasapt, Patravoot; Promthet, Supannee; Daduang, Jureerut; Teeramatwanich, Watchareporn; Kongyingyoes, Bunkerd; Chuerduangphui, Jureeporn; Ekalaksananan, Tipaya

doi:10.1016/j.jprot.2017.11.022

Cited by 26 publications

(18 citation statements)

References 42 publications

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“…The aberrant expression of PA28γ gene was suggested to be a prognostic biomarker of OSCC patients [11]. Peroxiredoxin-2 (PRDX-2) and zinc-alpha-2-glycoprotein (ZAG) were proved to be up-regulated in OSCC patients, which might be adopted as potential biomarkers for OSCC detection [12]. On the other hand, epigenetic factors also play a pivotal role in OSCC, such as DNA methylation.…”

Section: Introductionmentioning

confidence: 99%

KRT84 is a potential tumor suppressor and good prognosis signature of oral squamous cell carcinoma

Liu

Ren

2020

Bioscience Reports

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Aims: Oral squamous cell carcinoma (OSCC) is a common oral cancer; however, current therapeutic approaches still show limited efficacy. Our research aims to explore effective biomarkers related to OSCC. Main methods: Gene expression profiles of paired OSCC tumor and paracancerous samples from The Cancer Genome Atlas (TCGA) were analyzed. mRNA and protein levels of KRT84 in OSCC cell line HSC-3 were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. KRT84 protein levels in OSCC tumor samples of different stages were determined by immunohistochemistry. Overall survival (OS) of OSCC samples was evaluated and association of multiple factors with OS was assessed. Key findings: Compared with paracancerous samples, 4642 DEGs were identified in OSCC tumor samples. Among them, KRT84 expression level in OSCC tumor tissues was obviously decreased, which was validated in HSC-3 cells. KRT84 expression level showed decreasing tendency with the increase of tumor grade and stage. Patients with low KRT84 expression level had inferior OS independently of multiple factors. Besides, antigen processing and presentation pathway were significantly activated in OSCC samples with high KRT84 expression. Elevated KRT84 mRNA as well as protein levels were confirmed by RT-qPCR and Western blot in OSCC and normal cell lines, and immunohistochemistry in OSCC tumor and paracancerous tissues. Significance: Our study suggests KRT84 as a tumor suppressor and good prognostic indicator for OSCC, which might be significant for OSCC diagnosis and treatment.

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Section: Introductionmentioning

confidence: 99%

KRT84 is a potential tumor suppressor and good prognosis signature of oral squamous cell carcinoma

Liu

Ren

2020

Bioscience Reports

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“…Regarding sampling methods, HPV DNA load, and HPV antibodies can be detected in plasma as well as in saliva ( 23 , 24 , 26 , 72 ). Wang et al demonstrated that HPV DNA could be detected in the plasma of 86% of the patients, compared to only 40% of the saliva of these same patients, indicating that plasma would be more informative to diagnose HPV-associated tumors despite the need for invasive sampling ( 24 ).…”

Section: Resultsmentioning

confidence: 99%

“…As already mentioned, human papillomavirus (HPV) and Epstein-Barr virus (EBV) are identified viral risk factors for head and neck neoplasms (72). Since HPV-16 accounts for >90% of HPV-DNA positive head and neck squamous cell carcinomas (HNSCC), it is regarded as the predominant HPV type in these specific malignancies (73).…”

Section: Human Papillomavirus Dna Loadmentioning

confidence: 99%

A Literature Review of the Potential Diagnostic Biomarkers of Head and Neck Neoplasms

et al. 2020

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Head and neck neoplasms have a poor prognosis because of their late diagnosis. Finding a biomarker to detect these tumors in an early phase could improve the prognosis and survival rate. This literature review provides an overview of biomarkers, covering the different-omics fields to diagnose head and neck neoplasms in the early phase. To date, not a single biomarker, nor a panel of biomarkers for the detection of head and neck tumors has been detected with clinical applicability. Limitations for the clinical implementation of the investigated biomarkers are mainly the heterogeneity of the study groups (e.g., small population in which the biomarker was tested, and/or only including high-risk populations) and a low sensitivity and/or specificity of the biomarkers under study. Further research on biomarkers to diagnose head and neck neoplasms in an early stage, is therefore needed.

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“…Peroxiredoxin (PRDX‐2) is an endogenous thiol‐dependent antioxidant enzyme involved in the regulation of cell growth, differentiation and apoptosis; salivary PRDX‐2 expression is increased in OSCC . PRDX‐2, ANXA‐1 and ZA2G may thus function as putative markers for early OSCC detection, irrespective of aetiology;Table . Further verification is required to determine their discriminatory potential, however, especially as salivary PRDX‐2 expression may increase in inflammatory oral and systemic disease.…”

Section: Proteomic/peptidomic Biomarker Combinationsmentioning

confidence: 99%

“Search less, verify more”—Reviewing salivary biomarkers in oral cancer detection

Adeoye

Brennan

Thomson

2020

J Oral Pathology Medicine

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Oral squamous cell carcinoma is one of the commonest head and neck malignancies with approximately 350 000 cases reported annually and a mortality rate of 50% often attributed to late clinical presentation. Due to the close relationship between saliva bio‐fluid and tumour lesions, optimizing salivary biomarkers for disease detection and screening provides a major new research direction in diagnostic oral oncology. As inter‐tumour heterogeneity and intra‐tumour heterogeneity are common within oral cavity neoplasms, it is unlikely that a single diagnostic or “risk‐stratifying” saliva biomarker will suffice for universal translation to clinical practice. Therefore, this article highlights a number of promising saliva biomarker combinations for oral cavity cancer detection that require further research and validation to determine their true diagnostic potential.

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Peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic approaches

Cited by 26 publications

References 42 publications

KRT84 is a potential tumor suppressor and good prognosis signature of oral squamous cell carcinoma

KRT84 is a potential tumor suppressor and good prognosis signature of oral squamous cell carcinoma

A Literature Review of the Potential Diagnostic Biomarkers of Head and Neck Neoplasms

“Search less, verify more”—Reviewing salivary biomarkers in oral cancer detection

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