Peroxiredoxin 2 Inhibits Granulosa Cell Apoptosis During Follicle Atresia Through the NFKB Pathway in Mice1

Yang, Shuhong; Luo, Aiyue; Xing, Hao; Lai, Zhiwen; Ding, Ting; Ma, Xiangyi; Mayinuer, Maitituohe; Shen, Wei; Wang, Xi; Lu, Yao; Ma, Ding; Wang, Shixuan

doi:10.1095/biolreprod.110.087569

Cited by 36 publications

(37 citation statements)

References 33 publications

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“…25 After 2 h of treatment with 200 mM H 2 O 2 , the rate of apoptosis in GCs transfected with Cmv6-Egr1 at 48 h after transfection showed an even stronger increase (30.2%) compared with the vector group (18.5%) (Fig. 3B and D).…”

Section: Up-regulation Of Egr1 Significantly Promoted Gc Apoptosismentioning

confidence: 88%

“…Considering that EGR1 can be stimulated by multiple factors such as UV radiation and ROS to regulate oxidative stress and apoptosis 24 and that ROS have been reported to play an important role in GCs apoptosis during ovarian aging, 25 the pro-apoptotic effect of EGR1 was also evaluated under stimulation with H 2 O 2 , a type of ROS that we have previously shown to induce GC apoptosis. 25 After 2 h of treatment with 200 mM H 2 O 2 , the rate of apoptosis in GCs transfected with Cmv6-Egr1 at 48 h after transfection showed an even stronger increase (30.2%) compared with the vector group (18.5%) (Fig.…”

Section: Up-regulation Of Egr1 Significantly Promoted Gc Apoptosismentioning

confidence: 99%

“…Identification of follicle types and scores and classification of follicles were based on the criteria used in our previously published articles. 25 …”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Total RNA was extracted, digested and reverse-transcribed into cDNA and then Real-time PCR was carried out according to our previously published report. 25 The glyceraldehyde-3 phosphate dehydrogenase (Gapdh) gene was used as an internal control for Egr1 template normalization. The primers were synthesized by TIANYI HUIYUAN (Wuhan), with the following sequences (5 0 to 3 0 ; sense and antisense, respectively):…”

Section: Rna Extraction and Quantitative Real-time Pcrmentioning

confidence: 99%

“…The proportions of EdU-positive cells were calculated based on our previous criteria. 25 Experiments were repeated 3 times.…”

Section: Edu Assaymentioning

confidence: 99%

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Age-associated up-regulation of EGR1 promotes granulosa cell apoptosis during follicle atresia in mice through the NF-κB pathway

et al. 2016

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Follicular atresia is the main process responsible for the loss of follicles and oocytes from the ovary, and it is the root cause of ovarian aging. Apoptosis of granulosa cells (GCs) is the cellular mechanism responsible for follicular atresia in mammals. Recent advances have highlighted fundamental roles for EGR1 in agerelated diseases via the induction of apoptosis. In the present study, we found that the expression of EGR1 was significantly increased in aged mouse ovaries compared with young ovaries. Immunohistochemical analysis revealed strongly positive EGR1 staining in atretic follicles, especially in apoptotic granulosa cells. We further showed that EGR1 up-regulation in mouse primary granulosa cells inhibited cell proliferation and promoted apoptosis. In addition, the promotion of apoptosis in GCs by EGR1 increases over time and with reactive oxygen species (ROS) stimulation. Our mechanistic study suggested that EGR1 regulates GC apoptosis in a mitochondria-dependent manner and that this mainly occurs through the NF-kB signaling pathway. In conclusion, our results suggested that age-related up-regulation of EGR1 promotes GC apoptosis in follicle atresia during ovarian aging.

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Section: Up-regulation Of Egr1 Significantly Promoted Gc Apoptosismentioning

confidence: 88%

Section: Up-regulation Of Egr1 Significantly Promoted Gc Apoptosismentioning

confidence: 99%

“…Identification of follicle types and scores and classification of follicles were based on the criteria used in our previously published articles. 25 …”

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Rna Extraction and Quantitative Real-time Pcrmentioning

confidence: 99%

“…The proportions of EdU-positive cells were calculated based on our previous criteria. 25 Experiments were repeated 3 times.…”

Section: Edu Assaymentioning

confidence: 99%

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Age-associated up-regulation of EGR1 promotes granulosa cell apoptosis during follicle atresia in mice through the NF-κB pathway

et al. 2016

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A novel form of chondrocyte stress is triggered by a COMP mutation causing pseudoachondroplasia

et al. 2011

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Pseudoachondroplasia (PSACH) results from mutations in cartilage oligomeric matrix protein (COMP) and the p.D469del mutation within the type III repeats of COMP accounts for approximately 30% of PSACH. To determine disease mechanisms of PSACH in vivo, we introduced the Comp D469del mutation into the mouse genome. Mutant animals were normal at birth but grew slower than their wild-type littermates and developed short-limb dwarfism. In the growth plates of mutant mice chondrocyte columns were reduced in number and poorly organized, while mutant COMP was retained within the endoplasmic reticulum (ER) of cells. Chondrocyte proliferation was reduced and apoptosis was both increased and spatially dysregulated. Previous studies on COMP mutations have shown mutant COMP is co-localized with chaperone proteins, and we have reported an unfolded protein response (UPR) in mouse models of PSACH-MED (multiple epiphyseal dysplasia) harboring mutations in Comp (T585M) and Matn3, Comp etc (V194D). However, we found no evidence of UPR in this mouse model of PSACH. In contrast, microarray analysis identified expression changes in groups of genes implicated in oxidative stress, cell cycle regulation, and apoptosis, which is consistent with the chondrocyte pathology. Overall, these data suggest that a novel form of chondrocyte stress triggered by the expression of mutant COMP is central to the pathogenesis of PSACH. Hum Mutat 33:218–231, 2012. © 2011 Wiley Periodicals, Inc.

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ACT001 can prevent and reverse tamoxifen resistance in human breast cancer cell lines by inhibiting NF‐κB activation

Jin

Jia

Shi

et al. 2018

J of Cellular Biochemistry

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Endocrine therapy is one of the main treatments for estrogen receptor–positive breast cancers. Tamoxifen is the most commonly used drug for endocrine therapy. However, primary or acquired tamoxifen resistance occurs in a large proportion of breast cancer patients, leading to therapeutic failure. We found that the combination of tamoxifen and ACT001, a nuclear factor‐κB (NF‐κB) signaling pathway inhibitor, effectively inhibited the proliferation of both tamoxifen‐sensitive and tamoxifen‐resistant cells. The tamoxifen‐resistant cell line MCF7R/LCC9 showed active NF‐κB signaling and high apoptosis‐related gene transcription, especially for antiapoptotic genes, which could be diminished by treatment with ACT001. These results demonstrate that ACT001 can prevent and reverse tamoxifen resistance by inhibiting NF‐κB activation.

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Peroxiredoxin 2 Inhibits Granulosa Cell Apoptosis During Follicle Atresia Through the NFKB Pathway in Mice1

Cited by 36 publications

References 33 publications

Age-associated up-regulation of EGR1 promotes granulosa cell apoptosis during follicle atresia in mice through the NF-κB pathway

Age-associated up-regulation of EGR1 promotes granulosa cell apoptosis during follicle atresia in mice through the NF-κB pathway

A novel form of chondrocyte stress is triggered by a COMP mutation causing pseudoachondroplasia

ACT001 can prevent and reverse tamoxifen resistance in human breast cancer cell lines by inhibiting NF‐κB activation

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