Peroxisome Protein Prediction in Drosophila melanogaster

Anderson-Baron, Matthew; Simmonds, Andrew

doi:10.1007/978-981-13-2233-4_10

Cited by 6 publications

(5 citation statements)

References 71 publications

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“…Peroxisomes fight bacterial infections through classic innate immune signals, and studies have shown that the reduction of peroxisome function impairs the turnover of the oxidative burst necessary for fighting infection. Further research on peroxisomes and their role in the immune system is needed [55]. The downregulated DEGs were also enriched in ABC transporters.…”

Section: Analysis Of Other Pathwaysmentioning

confidence: 99%

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Ce-jie

Gao

et al. 2020

Parasites Vectors

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Background: Dengue virus (DENV) is a flavivirus transmitted by mosquitoes that is prevalent in tropical and subtropical countries and has four serotypes (DENV1-4). Aedes aegypti, as the main transmission vector of DENV, exhibits strong infectivity and transmission. With the aim of obtaining a better understanding of the Ae. aegypti-DENV interaction, the transcriptome changes in DENV-2-infected Aag2 cells were studied to describe the immune responses of mosquitoes using the Ae. aegypti Aag2 cell line as a model. Methods: RNAseq technology was used to sequence the transcripts of the Ae. aegypti Aag2 cell line before and after infection with DENV-2. A bioinformatics analysis was then performed to assess the biological functions of the differentially expressed genes, and the sequencing data were verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The transcriptome analysis generated 8866 unigenes that were found in both groups, 225 unigenes that were only found in the infection group, and 683 unigenes that only existed in the control group. A total of 1199 differentially expressed genes, including 1014 upregulated and 185 downregulated genes, were identified. The bioinformatics analysis showed that the differentially expressed genes were mainly involved in the longevity regulating pathway, circadian rhythm, DNA replication, and peroxisome, purine, pyrimidine, and drug metabolism. The qRT-PCR verification results showed the same trend, which confirmed that the expression of the differentially expressed genes had changed, and that the transcriptome sequencing data were reliable. Conclusions: This study investigated the changes in the transcriptome levels in the DENV-2-infected Ae. aegypti Aag2 cell line, which provides a faster and effective method for discovering genes related to Ae. aegypti pathogen susceptibility. The findings provide basic data and directions for further research on the complex mechanism underlying host-pathogen interactions.

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Section: Analysis Of Other Pathwaysmentioning

confidence: 99%

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Ce-jie

Gao

et al. 2020

Parasites Vectors

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“…multiple potential orthologs for many of the peroxisome proteins, based on the presence of a PTS1 domain or homology to known peroxisome proteins in yeast or mammals. Baron et al (2016) systematically tested the peroxisome localization of each of these predicted peroxisomal proteins encoded by these, confirming the subset that was targeted to peroxisomes, and likely represented bona-fide peroxisome factors (Tables 2-4; reviewed in Anderson- Baron and Simmonds, 2018). Notably, in S2 cells, Pex3 and Pex19 were localized to peroxisomes at a level less than was expected, but functional tests suggested this was likely due to the epitope tags used for visualization (Baron et al, 2016).…”

Section: Identification Of Other Peroxisome Factorsmentioning

confidence: 80%

Rosy Beginnings: Studying Peroxisomes in Drosophila

Pridie

Ueda

Simmonds

2020

Front. Cell Dev. Biol.

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Research using the fruit fly Drosophila melanogaster has traditionally focused on understanding how mutations affecting gene regulation or function affect processes linked to animal development. Accordingly, flies have become an essential foundation of modern medical research through repeated contributions to our fundamental understanding of how their homologs of human genes function. Peroxisomes are organelles that metabolize lipids and reactive oxygen species like peroxides. However, despite clear linkage of mutations in human genes affecting peroxisomes to developmental defects, for many years fly models were conspicuously absent from the study of peroxisomes. Now, the few early studies linking the Rosy eye color phenotype to peroxisomes in flies have been joined by a growing body of research establishing novel roles for peroxisomes during the development or function of specific tissues or cell types. Similarly, unique properties of cultured fly Schneider 2 cells have advanced our understanding of how peroxisomes move on the cytoskeleton. Here, we profile how those past and more recent Drosophila studies started to link specific effects of peroxisome dysfunction to organ development and highlight the utility of flies as a model for human peroxisomal diseases. We also identify key differences in the function and proliferation of fly peroxisomes compared to yeast or mammals. Finally, we discuss the future of the fly model system for peroxisome research including new techniques that should support identification of additional tissue specific regulation of and roles for peroxisomes.

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“…Peroxisomes ght bacterial infections through classic innate immune signals, and studies have shown that the reduction of peroxisome function impairs the turnover of the oxidative burst necessary for ghting infection. Further research on peroxisomes and their role in the immune system is needed [55]. The downregulated DEGs were also enriched in ABC transporters.…”

Section: Analysis Of Other Pathwaysmentioning

confidence: 99%

Transcriptome Analysis of Aedes aegypti Aag2 Cells in Response to Dengue Virus-2 Infection

Lan

Gao

et al. 2020

Preprint

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Background: Dengue virus (DENV) is a flavivirus transmitted by mosquitoes that is prevalent in tropical and subtropical countries and has four serotypes (DENV1-4). Aedes aegypti, as the main transmission vector of DENV, exhibits strong infectivity and transmission. With the aim of obtaining a better understanding of the Ae. aegypti-DENV interaction, the transcriptome changes in DENV-2-infected Aag2 cells were studied to describe the immune responses of mosquitoes using the Ae. aegypti Aag2 cell line as a model.Methods: RNAseq technology was used to sequence the transcripts of the Ae. aegypti Aag2 cell line before and after infection with DENV-2. A bioinformatics analysis was then performed to assess the biological functions of the differentially expressed genes, and the sequencing data were verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).Results: The transcriptome analysis generated 8866 unigenes that were found in both groups, 225 unigenes that were only found in the infection group, and 683 unigenes that only existed in the control group. A total of 1199 differentially expressed genes, including 1014 upregulated and 185 downregulated genes, were identified. The bioinformatics analysis showed that the differentially expressed genes were mainly involved in the longevity regulating pathway, circadian rhythm, DNA replication, and peroxisome, purine, pyrimidine, and drug metabolism. The qRT-PCR verification results showed the same trend, which confirmed that the expression of the differentially expressed genes had changed and that the transcriptome sequencing data were reliable.Conclusions: This study investigated the changes in the transcriptome levels in the DENV-2-infected Ae. aegypti Aag2 cell line, which provides a faster and effective method for discovering genes related to Ae. aegypti pathogen susceptibility. The findings provide basic data and directions for further research on the complex mechanism underlying host-pathogen interactions.

show abstract

Peroxisome Protein Prediction in Drosophila melanogaster

Cited by 6 publications

References 71 publications

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Rosy Beginnings: Studying Peroxisomes in Drosophila

Transcriptome Analysis of Aedes aegypti Aag2 Cells in Response to Dengue Virus-2 Infection

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