Peroxynitrite dominates sodium nitroprusside-induced apoptosis in human hepatocellular carcinoma cells

Quan, Yingyao; Liu, Yuhong; Lin, Chunmei; Wang, Xiaoping; Chen, Tongsheng

doi:10.18632/oncotarget.16164

Cited by 13 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on our recent finding that ONOO ─ from the reaction between NO and O2· − dominated the sodium nitroprusside (SNP)-induced apoptosis in HepG2 cells [ 49 ], we speculated that ONOO ─ might dominate the synergistic chemo-photothermal anti-cancer effect of nGO-PEG-ARS. As expected, FCM analysis with DHR 123 staining showed that nGO-PEG-ARS treatment for 6 h potently induced abundant ONOO ─ generation which was significantly enhanced by NIR irradiation (Figure 4C ).…”

Section: Resultsmentioning

confidence: 99%

“…ROS and ONOO ─ generations inside living cells were measured by FCM with an oxidation-sensitive probe 2′, 7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma, USA) and Dihydrorhodamine 123 (DHR 123, Wuhan, Aimeijie) as described previously [ 49 ]. DCF-DA is cleaved by nonspecific esterases and becomes highly fluorescent DCF upon oxidation by ROS.…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular NO level was quantified by using FCM analysis with DAF-FM DA staining as described previously [ 49 ]. HepG2 cells were cultured with nGO-PEG and nGO-PEG-ARS for the time indicated with or without 5 W/cm 2 of 808 nm NIR laser for 15min, and then the cells were stained with 5 μM DAF-FM DA for 30 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Artesunate-modified nano-graphene oxide for chemo-photothermal cancer therapy

et al. 2017

View full text Add to dashboard Cite

Poor water-solubility of artesunate (ARS) hampers its clinical application. We here covalently linked ARS to PEGylated nanographene oxide (nGO-PEG) to obtain ARS-modified nGO-PEG (nGO-PEG-ARS) with excellent photothermal effect and dispersibility in physiological environment. nGO-PEG-ARS induced reactive oxygen species (ROS) and peroxynitrite (ONOO─) generations. Although nGO-PEG with near-infrared (NIR) irradiation did not induce cytotoxicity, the photothermal effect of nGO-PEG under NIR irradiation enhanced not only cell uptake but also ONOO─ generation of nGO-PEG-ARS, resulting in the synergistic chemo-photothermal effect of nGO-PEG-ARS in killing HepG2 cells. Pretreatment with Fe(III) 5,10,15,20-tetrakis (4-sulfonatophenyl) porphyrinato chloride (FeTTPS, a ONOO─ scavenger) instead of antioxidant N-Acetyle-Cysteine (NAC, an ROS scavenger) significantly blocked the cytotoxicity of nGO-PEG-ARS with or without NIR irradiation, demonstrating that ONOO─ instead of ROS dominated the synergistic chemo-photothermal anti-cancer action of nGO-PEG-ARS. nGO-PEG-ARS with NIR irradiation resulted in a complete tumor cure within 15 days earlier than other treatment groups, and did not induce apparent histological lesion for the mice treated with nGO-PEG-ARS with or without NIR irradiation for 30 days, further proving the synergistic chemo-photothermal anti-cancer effect of nGO-PEG-ARS. Collectively, nGO-PEG-ARS is a versatile nano-platform for multi-modal synergistic cancer therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Artesunate-modified nano-graphene oxide for chemo-photothermal cancer therapy

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This finding is consistent with the previous report that SNP ( 500 lM) was toxic to mammalian cells. 33,37 However, it is interesting that there observed SNP induced cytotoxicity discrepancy between Acanthamoeba and HCECs. Exposure to 100 lM of SNP for 3 days induced only 30% of cytotoxicity on HCEC, whereas >90% of Acanthamoeba were killed under the same condition.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nitric Oxide onAcanthamoeba castellanii

Yim

Park

Jeong

et al. 2018

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Введення L-аргініну, напроти, не впливало на цей показник і запобігало підвищенню СОД активності під впливом CdCl2. Різні ефекти донорів NO на активність СОД можна пов'язати з появою АФК за введення SNP, під час спонтанного розкладання якого разом з NO утворюються також іони заліза, пероксид водню і супероксид-аніон (Quan et al, 2017).…”

Section: таблицяunclassified

The effect of nitrogen monoxide donors on the indexes of cadmium-induced oxidative stress in different rat tissues

2020

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

View full text Add to dashboard Cite

Oxidative stress is considered to be the main mechanism of cadmium ions toxic effect on the cells and is caused by cadmium, as a non-transition metal, indirectly. Oxidative damage to cells due to the action of cadmium ions is tissue-specific and is associated with the antioxidant system inhibition, free heme accumulation and essential metals substitution in metalloproteins. Nitrogen monoxide (NO) exhibits high affinity for heme and proteins and peptides sulfhydryl groups, known to be the main molecular targets for cadmium ions. Taking all the above-mentioned into account, the aim of this work was to study the effect of NO radicals donors on the prooxidant-antioxidant state of mammalian tissues under oxidative stress caused by cadmium chloride administration in vivo. Male Wistar rats weighing 160–200 g were used in the study. CdCl2 was administered subcutaneously at a dose of 14 mg/kg body weight. The direct donor of the NO radical sodium nitroprusside (SNP, 1 mg/kg mass) and the substrate of the NO synthase reaction L-arginine (600 mg/kg mass) were administered intraperitoneally. In order to study the corrective action, donors of the NO radical were injected 0.5 h before the cadmium salt. The objects of investigation were blood plasma and liver, kidneys and spleen homogenates of rats. The cadmium chloride treatment caused a number of prooxidant-antioxidant balance disorders, most of which were revealed a day after injection. The accumulation of lipid peroxidation products was found in rat serum, liver, and spleen. The enhancement of prooxidant processes in these tissues may originate from cadmium ions and hemolysis products entry. In the antioxidant system, significant changes were observed under cadmium action only in the liver: an increase in the reduced glutathione content and SOD activity and a decrease in catalase activity. The precursor of nitric oxide L-arginine did not change the basal level of prooxidant-antioxidant parameters, and in most cases did not affect their dynamics in the organs studied after cadmium chloride administration. A direct NO donor, sodium nitroprusside, acted in liver and spleen mostly as a prooxidant. In liver, the injection of only nitroprusside, as well as the combined administration of SNP and CdCl2, led to free radical processes activation just in two hours. In spleen, the combined treatment by SNP and cadmium salt also caused an earlier development of oxidative stress, as witnessed by an increase in lipid hydroperoxides level and a decrease in reduced glutathione content. Therefore, the injection of a direct NO donor, sodium nitroprusside, and a substrate of NO synthase, L-arginine, in selected doses has insignificant corrective action on cadmium-induced oxidative stress in the liver, kidneys and spleen. However, in blood both donors of NO effectively prevented the accumulation of lipid peroxidation products under CdCl2 treatment; in addition, L-arginine significantly reduced the lactate dehydrogenase release, which may indicate blood cells and blood vessels protection from the damage caused by cadmium ions.

show abstract

Peroxynitrite dominates sodium nitroprusside-induced apoptosis in human hepatocellular carcinoma cells

Cited by 13 publications

References 59 publications

Artesunate-modified nano-graphene oxide for chemo-photothermal cancer therapy

Artesunate-modified nano-graphene oxide for chemo-photothermal cancer therapy

Effect of Nitric Oxide onAcanthamoeba castellanii

The effect of nitrogen monoxide donors on the indexes of cadmium-induced oxidative stress in different rat tissues

Contact Info

Product

Resources

About