Peroxynitrite irreversibly decreases diastolic and systolic function in cardiac muscle

Bcl-xL Augmentation Potentially Reduces Ischemia/Reperfusion Induced Proximal and Distal Tubular Apoptosis and Autophagy

“…Renal sections were deparaffinized, rehydrated, and stained immunohistochemically for presence of in vivo markers of lipid peroxidation, 4-HNE-protein adducts, by incubation with a polyclonal antibody (Alpha Diagnostic International; San Antonio, TX) (29), with rabbit polyclonal anti-3-NT antibody (Alpha Diagnostic International) (28) and a autophagy marker of Beclin-1 (AnaSpec, Inc., San Jose, CA) diluted at 1:50. The percentage of beclin-1/autophagy was calculated as beclin-1-stained area/total areaϫ100% and analyzed by Adobe Photoshop 7.0.1 image software analysis.…”

“…Accordingly, we searched for renal deposition of 3-NT and 4-HNE adducts with a specific antibody to 3-NT and 4-HNE adducts (28,29). The results were illustrated in representative micrographs (Fig.…”

Peroxynitrite-induced cardiac depression: role of myofilament desensitization and cGMP pathway

“…The concentration of peroxynitrite in the syringe was routinely monitored before use by measuring the extinction coefficient at 303 nm after the addition of 5 Al of peroxynitrite solution to 995 Al of 1 N NaOH. Hearts were exposed to peroxynitrite essentially as previously described [12]. Briefly, the peroxynitrite solution was delivered into the aortic cannula via a catheter ( f 10 cm long) attached to a port.…”

Diprivan attenuates the cytotoxicity of nitric oxide in cultured human bronchial epithelial cells

“…Tyrosine nitration correlates with impaired cardiac [34] and pulmonary [35] functions. Although the exact mechanism by which Diprivan protects against NO-induced toxicity is not well studied, some investigators have shown that NO and NO derivatives may be inactivated by directly nitrating propofol [36,37].…”