Peroxyoxalate chemiluminescence detection with capillary liquid chromatography

Weber, Andrew J.; Grayeski, Mary Lynn

doi:10.1021/ac00137a017

Cited by 21 publications

(3 citation statements)

References 42 publications

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“…For example, peaks that elute between 13-16 min with UV detection have a peak width at half height of 0.64 min, while peaks in the CL electropherogram do not exhibit peak areas of this magnitude until after 30 min. This type of phenomenon has been well documented in the literature for CL detection and is called the "chemical band narrowing" effect [4,5,19,21]. The CL kinetics allow for an intense, rapidly decaying signal to be produced when the acridinium tagged analyte enters the reaction zone of the detector.…”

Section: Phe Ala-pro-arg-leu-arg-phe-tyr-ser-mentioning

confidence: 97%

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Investigation of acridinium labelling for chemiluminescence detection of peptides separated by capillary electrophoresis

Ruberto

Grayeski

1994

J Microcolumn Separations

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Abstract. An acridinium ester has been used to tag mixtures of peptides followed by separation by capillary electrophoresis with chemiluminescence detection. The labelling of the peptides was performed at the picomole level. The chemiluminescence reaction conditions were optimized for the acridinium tag resulting in CE separations performed on attomole amounts of tagged peptides. Baseline resolution was obtained for a separation of standard peptides. The detection sensitivity was found to improve when multiple acridinium labels were attached to the peptides. The effect of acridinium tagging on the migration rate of the peptides was also studied. The increased weight of the acridinium tagged analyte increased the run time and resolution of a separated tryptic digest without resulting in excessive band broadening.

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Section: Phe Ala-pro-arg-leu-arg-phe-tyr-ser-mentioning

confidence: 97%

“…Chemiluminescence detection is a sensitive measurement uniquely suited for the low volume, on-line detection needed for CE. The nature of the CL kinetic measurement allows for rapid and sensitive on-column detection in larger volume flow cells without a significant introduction of band broadening [4,5].…”

Section: Introductionmentioning

confidence: 99%

Investigation of acridinium labelling for chemiluminescence detection of peptides separated by capillary electrophoresis

Ruberto

Grayeski

1994

J Microcolumn Separations

View full text Add to dashboard Cite

show abstract

“…For any particular application, the best choice of reagent depends on the instrumental approach and the nature of the assay [83,[89][90][91][92]. For example, DNPO reacts at faster rates than TCPO and produces greater chemiluminescence intensities [93], but this is only an advantage if the detection manifold is configured so that the most intense emission occurs within the flow cell [89,90]. TDPO is much more soluble in acetonitrile and more stable in the presence of hydrogen peroxide [94].…”

Section: Peroxyoxalate Reagentsmentioning

confidence: 99%