Persistence and Leaching Potential of Microorganisms and Mineral N in Animal Manure Applied to Intact Soil Columns

Amin, M. G. Mostofa; Forslund, Anita; Bui, Xuan Thanh; Juhler, René K.; Petersen, Søren O.; Lægdsmand, Mette

doi:10.1128/aem.02506-12

Cited by 41 publications

(27 citation statements)

References 68 publications

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“…In addition, various common agricultural practices, such as storage practices, method of application, timing of application, and tillage practices, have been shown to impact bacterial survival and must be considered (Hutchison et al 2004b;Arrus et al 2006;Coelho et al 2007;Semenov et al 2009;Samarajeewa et al 2012;Amin et al 2013;Hoang et al 2013). While numerous studies have documented persistence of SALM, and other pathogens in soils amended with swine and bovine manures, fewer have evaluated bacterial survival on artificially drained glacial till-derived soils (Gessel et al 2004;Rogers et al 2011;Samarajeewa et al 2012;Hoang et al 2013;Garder et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Hruby

Soupir

Pederson

et al. 2018

Water Air Soil Pollut

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Minimizing the risks associated with manure-borne pathogenic microorganisms requires an understanding of microbial survival under realistic field conditions. The objective of this 3-year study was to assess the fate of Salmonella (SALM) and fecal indicator bacteria (FIB), E. coli (EC) and enterococci (ENT), in glacial tillderived soils, after application of poultry manure (PM) to cornfields under chisel-plowed (CP) or no-till (NT) management. From 2010 to 2012, soil samples were obtained each spring at 0-15-and 15-30-cm depths, to determine whether over-wintering of target bacteria had occurred. Sampling was followed by application of PM at low (PM1) and high (PM2) rates, based on nitrogen application goals. In 2012, soil samples were collected 21, 42, and 158 days after manure application (DAM), to assess the effects of time, application rates, and tillage on frequency of detection and concentrations of target bacteria. Despite dry conditions, all three target organisms were detected 158 DAM in 2012, and detection of these organisms in spring soil samples from manured plots in 2011 and 2012, nearly a full year after PM application, suggests that these organisms can persist in the soil environment long after application. The highest SALM concentration (790 cfu/g dry weight) and detection rate (25%) was found in PM2 plots 42 DAM. SALM were detected more frequently in CP plots (20%) compared to NT plots (5%). In contrast, tillage practices had no apparent effect on EC or ENT survival, as indicated by both soil, and decay rates estimated from tile-water bacteria concentrations. Decay rate constants (μ) ranged from 0.044 to 0.065 day−1 for EC and 0.010 to 0.054 day−1 for ENT. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Abstract Minimizing the risks associated with manureborne pathogenic microorganisms requires an understanding of microbial survival under realistic field conditions. The objective of this 3-year study was to assess the fate of Salmonella (SALM) and fecal indicator bacteria (FIB), E. coli (EC) and enterococci (ENT), in glacial till-derived soils, after application of poultry manure (PM) to cornfields under chisel-plowed (CP) or no-till (NT) management. From 2010 to 2012, soil samples were obtained each spring at 0-15-and 15-30-cm depths, to determine whether over-wintering of target bacteria had occurred. Sampling was followed by application of PM at low (PM1) and high (PM2) rates, based on nitrogen application goals. In 2012, soil samples were collected 21, 42, and 158 days after manure application (DAM), to assess the effects of time, application rates, and tillage on frequency of detection and concentrations of target bacteria. Despite dry conditions, all three target organisms were detected 158 DAM in 2012, and detection of these organisms in spring soil samples from manured plots in 2011 and 2012, nearly a full year after PM application, suggests that these or...

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Section: Introductionmentioning

confidence: 99%

Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Hruby

Soupir

Pederson

et al. 2018

Water Air Soil Pollut

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“…Manure is frequently applied for field fertilization and that requires the observance of the appropriate withdrawal period. Otherwise, a large load of pathogenic bacteria and viruses can be introduced to the soil, posing a major epidemiological threat (Amin et al, 2013). Microorganism survival in the soil environment is favored by high temperature and moisture (Boes et al, 2005;Ngole et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microbial contamination of feces and soil on a laying-hen farm depending on sampling site and season

Trawińska

Chmielowiec-Korzeniowska

Nowakowicz-Dębek

et al. 2016

R. Bras. Zootec.

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-The objective of the present study was to evaluate soil collected from a laying-hen farm and bird manure according to the season of the year and sampling site. Soil samples were taken at the poultry facility wall and at the distances of 15 m and 45 m from the building. Bird feces samples were collected inside the poultry house at the entrance and at ¼ and ½ length of the building. Soil and bird feces samples were evaluated by bacteriological qualitative and quantitative analyses. The largest bacterial load was determined in the samples taken at the poultry facility wall in December/January. Soil microbial contamination degree was low. The highest bacterial count in bird manure was found in the samples collected at ½ length of the hen house at the end of December/January. The qualitative study of bird feces showed the presence of E. coli bacteria all through the research period and Enterobacter spp. in the samples taken from July until September. Microbial contamination of soil environment and bird feces is most likely to be affected by winter period as at that time the highest microbial population can be determined. This fact may be linked to the prevailing climatic and microclimatic conditions.

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“…This accords with the leaching of bacteria in other studies (e.g. Saini et al ., ; Amin et al ., ) and with the leaching of components of dairy slurry in the sandy loam and loam (Glæsner et al ., ,b). The numbers recorded, however, depend on the experimental conditions (e.g.…”

Section: Resultsmentioning

confidence: 99%

Bacteria as transporters of phosphorus through soil

Glæsner

Bælum

Jacobsen

et al. 2015

European J Soil Science

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Summary The transport of phosphorus (P) from agricultural land has led to the eutrophication of surface waters worldwide, especially in areas with intensive animal production. In this research, we investigated the role of bacteria in the leaching of P through three agricultural soils with different textures, before and after the surface application of dairy slurry manure (Bos taurus L.). Leachates of intact soil columns (diameter 20 cm, height 20 cm) of loamy sand, sandy loam and loam were analysed for total bacterial cells measured by universal 16S rRNA genes, and compared with the P content of leachates. The number of 16S rRNA genes per bacterial cell was validated by real‐time polymerase chain reaction (PCR) with a universal 16S rRNA gene primer set. Enumeration of bacterial cells by DAPI staining of cells extracted by density gradient centrifugation of the sandy loam estimated 4.6 16S rRNA genes cell−1. Leaching of bacteria was in the range of 2.5–4.5 × 105 cells ml−1 prior to application of slurry to the three soil textures. After slurry application, leaching increased to 1.1 × 106 cells ml−1 in the loamy sand, 4.9 × 106 cells ml−1 in the sandy loam and 5.0 × 106 cells ml−1 in the loam. Based on the reported P content of soil bacteria, 0.3–1.8% of the total P leached was present in the bacterial biomass when no slurry was applied, whereas slurry application increased the leaching of P from the bacterial biomass to 3−7.9% of total P leached. Bacterial leaching was related to the particulate P fraction of leachates of the loamy sand and sandy loam, but was also related to both dissolved and particulate leachate fractions of the loam. These results indicate that bacterial transport might play a role in the leaching of P from soil where manure has been applied, but this mechanism of transport is generally not addressed when considering the leaching of P.

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Persistence and Leaching Potential of Microorganisms and Mineral N in Animal Manure Applied to Intact Soil Columns

Cited by 41 publications

References 68 publications

Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Evaluation of microbial contamination of feces and soil on a laying-hen farm depending on sampling site and season

Bacteria as transporters of phosphorus through soil

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