Persistence and transgenerational effect of plant-mediated RNAi in aphids

Coleman, Alexander D.; Wouters, Roland H. M.; Mugford, Sam T.; Hogenhout, Saskia A.

doi:10.1093/jxb/eru450

Cited by 91 publications

(104 citation statements)

References 36 publications

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“…Successes in inducing RNAi in aphids (Hemiptera: Aphididae) by introducing dsRNA in artificial diet and/or via in planta expression of dsRNA hairpins to control green peach aphid, Myzus persicae (Coleman et al, 2015;Pitino et al, 2011), the pea aphid Acyrthosiphon pisum (Mao et al, 2013;Mao and Zeng, 2012), and the grain aphid, Sitobion avenae , bolster the potential for achieving RNAi in stink bugs. Since stink bugs can have multiple generations per year (Pilkay, 2013), parental RNAi-based approaches could be particularly effective in controlling this pest complex.…”

Section: Introductionmentioning

confidence: 99%

“…Parental or transgenerational RNAi effects have been observed in both coleopteran (Bucher et al, 2002;Khajuria et al, 2015;Shukla and Palli, 2014) and hemipteran insects (Coleman et al, 2015;Liu and Kaufman, 2004;Mao et al, 2013;Paim et al, 2013). For example, Liu and Kaufman (2004) used pRNAi via injection of dsRNA into the adult female to investigate the developmental role of the hunchback gene in the milkweed bug, Oncopeltus fasciatus (Hemiptera: Lygaeidae).…”

Section: Introductionmentioning

confidence: 99%

“…For example, Liu and Kaufman (2004) used pRNAi via injection of dsRNA into the adult female to investigate the developmental role of the hunchback gene in the milkweed bug, Oncopeltus fasciatus (Hemiptera: Lygaeidae). While in most cases the parental RNAi applications have been carried out via injection, there are observations via oral and plant transgene delivery in the green peach aphid (Coleman et al, 2015). In WCR, parental RNAi (pRNAi) responses have been demonstrated by feeding adult female rootworms dsRNA targeting brahma (brm) and hunchback (Khajuria et al, 2015) genes involved in embryonic development.…”

Section: Introductionmentioning

confidence: 99%

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Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Fishilevich¹,

Vélez

Khajuria

et al. 2016

Insect Biochemistry and Molecular Biology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Fishilevich¹,

Vélez

Khajuria

et al. 2016

Insect Biochemistry and Molecular Biology

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“…Silencing salivary effectors in non-arthropod herbivores like nematodes has been shown to reduce their performance (40). Likewise, silencing salivary effectors in insects like aphids reduced their reproduction (41). These studies indicate that salivary components are key players in the plant-herbivore molecular battlefield, and hence their identification is a high priority.…”

mentioning

confidence: 97%

The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors

Jonckheere

Dermauw

Zhurov

et al. 2016

Molecular & Cellular Proteomics

102

128

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The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions.Here, the proteomic composition of saliva from T. urticae lines adapted to various host plants-bean, maize, soy, and tomato-was analyzed using a custom-developed feeding assay coupled with nano-LC tandem mass spectrometry. About 90 putative T. urticae salivary proteins were identified. Many are of unknown function, and in numerous cases belonging to multimembered gene families. RNAseq expression analysis revealed that many genes coding for these salivary proteins were highly expressed in the proterosoma, the mite body region that includes the salivary glands. A subset of genes encoding putative salivary proteins was selected for whole-mount in situ hybridization, and were found to be expressed in the anterior and dorsal podocephalic glands. Strikingly, host plant dependent expression was evident for putative salivary proteins, and was further studied in detail by micro-array based genome-wide expression profiling. This meta-analysis revealed for the first time the salivary protein repertoire of a phytophagous chelicerate. The availability of this salivary proteome will assist in unraveling the molecular interface between phytophagous mites and their host plants, and may ultimately facilitate the development of mite-resistant crops. Furthermore, the technique used in this study is a time-and resourceefficient method to examine the salivary protein composition of other small arthropods for which saliva or salivary glands cannot be isolated easily. The family of spider mites (Chelicerata: Acari: Tetranychidae) comprises well over 1000 species, including several that are important pests on crops, and about 0.9 billion euro is being spent annually for their control worldwide (1, 2). These minute herbivores-about 0.5 mm in size-use their stylets to pierce leaf mesophyll cells and to inject saliva, after which they suck out the cytoplasm. This results in cell death visible as chlorotic spots sometimes accompanied by necrosis, and ultimately in leaf abscission (3,4). Among the spider mites, the From the ‡Laboratory

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“…The non-cell autonomous effect of RNAi was confirmed by tracking distribution of fluorescently-labeled dsRNA in the aphid’s body after oral acquisition [24,26]. Importantly, persistent down-regulation of target genes was observed in the progeny of aphids that have been fed on transgenic plants expressing dsRNA [38]. …”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of RNAi-Based Methods to Down-Regulate Expression of Two Genes Expressed at Different Levels in Myzus persicae

Mulot

Boissinot

Monsion

et al. 2016

Viruses

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With the increasing availability of aphid genomic data, it is necessary to develop robust functional validation methods to evaluate the role of specific aphid genes. This work represents the first study in which five different techniques, all based on RNA interference and on oral acquisition of double-stranded RNA (dsRNA), were developed to silence two genes, ALY and Eph, potentially involved in polerovirus transmission by aphids. Efficient silencing of only Eph transcripts, which are less abundant than those of ALY, could be achieved by feeding aphids on transgenic Arabidopsis thaliana expressing an RNA hairpin targeting Eph, on Nicotiana benthamiana infected with a Tobacco rattle virus (TRV)-Eph recombinant virus, or on in vitro-synthesized Eph-targeting dsRNA. These experiments showed that the silencing efficiency may differ greatly between genes and that aphid gut cells seem to be preferentially affected by the silencing mechanism after oral acquisition of dsRNA. In addition, the use of plants infected with recombinant TRV proved to be a promising technique to silence aphid genes as it does not require plant transformation. This work highlights the need to pursue development of innovative strategies to reproducibly achieve reduction of expression of aphid genes.

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Persistence and transgenerational effect of plant-mediated RNAi in aphids

Abstract: HighlightThis work shows that the transgenerational effect of RNA interference in the green peach aphid Myzus persicae contributes to a 60% decline in aphid population growth.

Cited by 91 publications

References 36 publications

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors

Comparative Analysis of RNAi-Based Methods to Down-Regulate Expression of Two Genes Expressed at Different Levels in Myzus persicae

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