Persistence length of fascin-cross-linked actin filament bundles in solution and the in vitro motility assay

Takatsuki, Hideyo; Bengtsson, Elina; Månsson, Alf

doi:10.1016/j.bbagen.2014.01.012

Cited by 48 publications

(62 citation statements)

References 57 publications

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“…In contrast, the Diabased actin filaments of the caps were organized as prominent, distinct bundles. These bundles may stiffen the cap cortex further, since bundles are more rigid than single actin filaments in vitro (Bretscher, 1981;Takatsuki et al, 2014). Additionally, we found the bundles to localize to the base of folds within the cap, and to move away from each other and toward the cap circumference in an Arp2/3-dependent, but myosin-independent, process, coupled with cap growth and compartment formation.…”

Section: Discussionmentioning

confidence: 67%

Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage

Jiang

Harris

2019

Journal of Cell Biology

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Cell structure depends on the cortex, a thin network of actin polymers and additional proteins underlying the plasma membrane. The cell polarity kinase Par-1 is required for cells to form following syncytial Drosophila embryo development. This requirement stems from Par-1 promoting cortical actin caps that grow into dome-like metaphase compartments for dividing syncytial nuclei. We find the actin caps to be a composite material of Diaphanous (Dia)-based actin bundles interspersed with independently formed, Arp2/3-based actin puncta. Par-1 and Dia colocalize along extended regions of the bundles, and both are required for the bundles and for each other’s bundle-like localization, consistent with an actin-dependent self-reinforcement mechanism. Par-1 helps establish or maintain these bundles in a cortical domain with relatively low levels of the canonical formin activator Rho1-GTP. Arp2/3 is required for displacing the bundles away from each other and toward the cap circumference, suggesting interactions between these cytoskeletal components could contribute to the growth of the cap into a metaphase compartment.

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Section: Discussionmentioning

confidence: 67%

Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage

Jiang

Harris

2019

Journal of Cell Biology

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“…The measured microtubule persistence length was an order of magnitude greater than that of actin filaments; nevertheless, it is much lower than the accepted values for microtubules of 1 − 10 mm in recent literature (7). Since then, microtubule and actin flexural rigidity (therefore persistence length) has been further estimated from dynamic video images using various techniques, including spectral analysis of thermally fluctuating filaments (3,(8)(9)(10)(11), end fluctuations (12,13), and tangent correlations (11,14,15). There have also been non-equilibrium measurements, including hydrodynamic flow (8,12,16), optical trapping (17)(18)(19)(20)(21), and atomic force microscopy (22).…”

Section: Introductionmentioning

confidence: 85%

Measurement of the Persistence Length of Cytoskeletal Filaments using Curvature Distributions

Wisanpitayakorn

Mickolajczyk

Hancock

et al. 2018

Preprint

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Cytoskeletal filaments such as microtubules and actin filaments play important roles in the mechanical integrity of cells and the ability of cells to respond to their environment. Measuring the mechanical properties of cytoskeletal structures is crucial for gaining insight into intracellular mechanical stresses and their role in regulating cellular processes. One of the ways to characterize these mechanical properties is by measuring their persistence length, the average length over which filaments stay straight. There are several approaches in the literature for measuring filament deformations, including Fourier analysis of images obtained using fluorescence microscopy. Here, we show how curvature distributions can be used as an alternative tool to quantify bio-filament deformations, and investigate how the apparent stiffness of filaments depends on the resolution and noise of the imaging system. We present analytical calculations of the scaling curvature distributions as a function of filament discretization, and test our predictions by comparing Monte Carlo simulations to results from existing techniques. We also apply our approach to microtubules and actin filaments obtained from in vitro gliding assay experiments with high densities of non-functional motors, and calculate the persistence length of these filaments. The presented curvature analysis is significantly more accurate compared to existing approaches for small data sets, and can be readily applied to both in vitro or in vivo filament data through the use of an ImageJ plugin we provide.1 not peer-reviewed)

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“…On the other hand, more flexible polymers diffuse more efficiently in the tumor tissue, which leads to their increased tumor accumulation. 3,4 The importance of the chain conformation of several biologically relevant macromolecules such as actin 5 or DNA 6 on their functionality has been reported in literature as well. DNA compaction is, probably, the most spectacular example of conformation changes as the DNA persistence length strongly decreases after complexation with mono or divalent ions.…”

mentioning

confidence: 96%

Conformational properties of biocompatible poly(2-ethyl-2-oxazoline)s in phosphate buffered saline

et al. 2018

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Persistence length of fascin-cross-linked actin filament bundles in solution and the in vitro motility assay

Abstract: The findings give general insight into mechanics of cytoskeletal polymers that interact with molecular motors, aid rational development of nanotechnological applications and have implications for structure and in vivo functions of fascin-actin bundles.

Cited by 48 publications

References 57 publications

Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage

Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage

Measurement of the Persistence Length of Cytoskeletal Filaments using Curvature Distributions

Conformational properties of biocompatible poly(2-ethyl-2-oxazoline)s in phosphate buffered saline

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