Persistence of catalytic activity of fungal phosphatases incubated in tropical soils

A novel isotope pool dilution approach to quantify gross rates of key abiotic and biological processes in the soil phosphorus cycle

“…However, adequate abiotic controls with no contribution of biological processes have remained a major obstacle in measuring soil P dynamics with radiotracers, both in IEK and IPD experiments. Procedures in earlier studies ranged from short-term assays with no inhibitor addition as often performed in IEK assays Oehl et al, 2001b), to amendments of HgCl 2 , sodium azide, toluene or chloroform, and gamma irradiation or repeated autoclaving (Kellogg et al, 2006;Bünemann et al, 2007;Oehl et al, 2001b;Achat et al, 2010).…”

“…2 of IPD assays. The commonly used soil water extractions target only a small fraction of this target pool, whereas standard soil P extractants, such as Olsen, Mehlich 3 or Bray 1, extract a larger fraction (Kleinman et al, 2001) and, therefore, are suggested to be better suited to extract the rapidly exchanging P i pool (Kellogg et al, 2006).…”

“…Consequently, the litter protocol cannot be directly transferred to soil studies. In the present study we developed an IPD protocol to assess soil P dynamics, based on the previous work for litter by Mooshammer et al (2012) and soils by Kellogg et al (2006). The protocol is based on IPD theory (Kirkham and Bartholomew, 1954;Di et al, 2000) applied to parallel incubations of live and sterile soil with 33 P i tracer addition.…”

Fate of insecticidalBacillus thuringiensisCry protein in soil: differences between purified toxin and biopesticide formulation

“…Adsorbed proteins are largely immobilised, and adsorption has various consequences. Adsorption is thought to confer both physical and chemical protection against microbial breakdown, although recent studies of fungal phosphatases indicate that this may not always be the case . Conformational changes due to electrostatic and hydrophobic interactions may modify the biological properties of the proteins.…”

Phosphorus use efficiency in conservation agricultural system: impact of organic restitution

“…However, organic phosphorus also occurs in soils and its mineralization offers another important potential source of phosphorus for plants . enzymes from the loss of activity they naturally experienced in solution (KEDI et al, 2013a(KEDI et al, , 2013bPASCUAL et al, 2002). This stabilization process makes these soils potentially prone to the mineralization of organic phosphorus (Po) for plant nutrition.…”