Persistence of <i>Tilapia tilapinevirus</i> in fish rearing and environmental water and its ability to infect cell line

Yamkasem, Jidapa; Prasartset, Tharinthon; Tattiyapong, Puntanat; Sirikanchana, Kwanrawee; Mongkolsuk, Skorn; Soto, Esteban; Surachetpong, Win

doi:10.1111/jfd.13593

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…The samples were then immediately processed for RNA isolation by filtered through 0.45 μM MF‐Millipore™ mixed cellulose esters filter (Sigma‐Aldrich, USA). The RNA template was isolated from the membrane using the Quick‐RNA™ Fecal/Soil Microbe Microprep Kit (Zymo Research, California), as described in a previously published protocol (Yamkasem et al, 2022), and cDNA was subsequently synthesized. We used the optimized ddPCR protocol described in detail in section 2.3, while the same cDNA and primer concentration was tested with the RT‐qPCR assay for a comparative evaluation of both methods of TiLV detection.…”

Section: Methodsmentioning

confidence: 99%

A specific and sensitive droplet digital polymerase chain reaction assay for the detection of tilapia lake virus in fish tissue and environmental samples

Shahi

Prasartset

Surachetpong

2023

Journal of Fish Diseases

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Tilapia lake virus (TiLV) causes high mortality in farmed and wild tilapia in various countries. We developed a highly specific and sensitive droplet digital polymerase chain reaction (ddPCR) assay to detect and quantify TiLV. The ddPCR assay could detect the virus at a lower threshold than the reverse transcription‐quantitative polymerase reaction (RT‐qPCR) method, and the sensitivity of the ddPCR assay was 10‐fold higher. The diagnostic sensitivity and specificity of the ddPCR assay were 100% and did not cross‐react with tilapia tissues infected with Tilapia parvovirus, Infectious spleen and kidney necrosis virus, Aeromonas hydrophila, Streptococcus agalactiae, S. iniae and Francisella noatunensis. The assay reproducibility was demonstrated by a high correlation coefficient of 0.998, and the inter‐assay coefficients of variability indicated that the ddPCR assay exhibited low variability within and between measurements. The detection limit of the TiLV ddPCR assay was 100 fg cDNA, which is equal to 3.3 copies of TiLV. Furthermore, the ddPCR assay could detect TiLV in mucus, water and infected tissue samples and the lowest copy number of TiLV detected in water samples by the ddPCR assay was 7.9 ± 0.99 copies/reaction The results of the clinical samples tested for TiLV revealed that the ddPCR assay had a relatively higher detection rate than the RT‐qPCR method. Overall, the ddPCR method offers a highly promising approach for the absolute quantification of TiLV in carrier fish and samples from the environment with low viral concentrations.

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Section: Methodsmentioning

confidence: 99%

A specific and sensitive droplet digital polymerase chain reaction assay for the detection of tilapia lake virus in fish tissue and environmental samples

Shahi

Prasartset

Surachetpong

2023

Journal of Fish Diseases

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“…In epidemiological models, the proportion of infectious tilapia appears to be maximal after 5 days of contamination, decreasing but not disappearing after 16 days (Yang et al 2018), and fish without clinical signs also tested positive for TiLV (Senapin et al 2018). Considering the survival of the virus outside the host, water disinfection for 3-5 days before stocking new tilapia (Yamkasem et al 2022) could be an effective biosecurity measure, however, considering the genetic resistance shown in our study, in farming areas where the risk of TiLVD is high, the selected resistant strain could be an efficient preventive measure.…”

Section: Discussionmentioning

confidence: 99%

Resistance of Indonesian tilapia strains, Oreochromis niloticus to Tilapia Lake Virus Disease (TiLVD)

Taukhid,

Lusiastuti,

Novita

et al. 2024

Bulletin of the European Association of Fish Pathologists

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This study aims to evaluate the resistance of six Indonesian tilapia strains to TiLV disease and assess the impact of mimicked daily water temperature fluctuations on the disease severity. Six widely cultured Indonesian tilapia (Oreochromis niloticus) strains were evaluated for resistance to tilapia lake virus disease (TiLVD). Resistance to TiLVD was assessed using two artificial infection methods, cohabitation and intraperitoneal (IP) injection. Fish infected with TiLVD exhibited behavioural, clinical and mortality patterns associated with TiLV disease. Virus presence was also confirmed molecularly. All six tilapia strains were susceptible to TiLV infection, causing disease and mortality. The range of mortality for cohabitation was 1.67% - 28.33% and for IP injection was 16.67% - 61.67%. The most resistant tilapia strain to TiLV disease was Gesit, followed by Best, Red/Albino, Nirwana, Non-specific and Srikandi. Daily temperature variation results in higher significant virus-induced mortality pattern among tilapia strains. These findings can be used to guide the selection of appropriate tilapia strain and temperature control to mitigate the adverse effects of TiLV associated disease.

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Tilapia lake virus infection disrupts the gut microbiota of red hybrid tilapia (Oreochromis spp.)

Paimeeka,

Tangsongcharoen,

Lertwanakarn

et al. 2024

Aquaculture

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Persistence of Tilapia tilapinevirus in fish rearing and environmental water and its ability to infect cell line

Abstract: Tilapia tilapinevirus, also known as Tilapia Lake Virus (TiLV), is a highrisk emerging RNA virus in cultured and wild tilapia (Oreochromis

Cited by 4 publications

References 46 publications

A specific and sensitive droplet digital polymerase chain reaction assay for the detection of tilapia lake virus in fish tissue and environmental samples

A specific and sensitive droplet digital polymerase chain reaction assay for the detection of tilapia lake virus in fish tissue and environmental samples

Resistance of Indonesian tilapia strains, Oreochromis niloticus to Tilapia Lake Virus Disease (TiLVD)

Tilapia lake virus infection disrupts the gut microbiota of red hybrid tilapia (Oreochromis spp.)

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