Persistence of Klebsiella pneumoniae ST258 as the predominant clone of carbapenemase-producing Enterobacteriaceae in post-acute-care hospitals in Israel, 2008-13

Adler, Amos; Hussein, Omar; Ben‐David, Debby; Masarwa, Samira; Navon-Venezia, Shiri; Schwaber, Mitchell J.; Carmeli, Yehuda

doi:10.1093/jac/dku333

Cited by 58 publications

(36 citation statements)

References 22 publications

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“…This was followed by global reports of ST258 among K. pneumoniae isolates with bla KPC from countries such as Greece (126), Norway, Sweden (127), Italy (128), Poland (129), Canada (130), Brazil (131), and South Korea (132), suggesting that this ST had characteristics of international multidrug-resistant high-risk clones. Recent reports from Israel (133) and Italy (134) demonstrated the endemicity and persistence of CC258 over time while remaining the predominant clone among K. pneumoniae isolates with bla KPC . Interestingly, Israel has seen an overall dramatic decrease in the incidence of KPCs among K. pneumoniae isolates, but ST258 still remains the most predominant clone (133).…”

Section: Initial Studies Pertaining To K Pneumoniae St258mentioning

confidence: 99%

“…Recent reports from Israel (133) and Italy (134) demonstrated the endemicity and persistence of CC258 over time while remaining the predominant clone among K. pneumoniae isolates with bla KPC . Interestingly, Israel has seen an overall dramatic decrease in the incidence of KPCs among K. pneumoniae isolates, but ST258 still remains the most predominant clone (133).…”

Section: Initial Studies Pertaining To K Pneumoniae St258mentioning

confidence: 99%

“…There were sharp increases in the numbers of cases and outbreaks in Israel during 2006 to 2007 in acute-care hospitals, which were driven primarily by ST258. After the nationwide implementation of infection control guidelines during the late 2000s to limit the spread of K. pneumoniae with bla KPC , there has been a steep decline in the incidence of infections due to ST258 throughout Israel (133).…”

Section: Recent Developments Pertaining To K Pneumoniae St258mentioning

confidence: 99%

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The Role of Epidemic Resistance Plasmids and International High-Risk Clones in the Spread of Multidrug-Resistant Enterobacteriaceae

2015

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SUMMARY Escherichia coli sequence type 131 (ST131) and Klebsiella pneumoniae ST258 emerged in the 2000s as important human pathogens, have spread extensively throughout the world, and are responsible for the rapid increase in antimicrobial resistance among E. coli and K. pneumoniae strains, respectively. E. coli ST131 causes extraintestinal infections and is often fluoroquinolone resistant and associated with extended-spectrum β-lactamase production, especially CTX-M-15. K. pneumoniae ST258 causes urinary and respiratory tract infections and is associated with carbapenemases, most often KPC-2 and KPC-3. The most prevalent lineage within ST131 is named fimH30 because it contains the H30 variant of the type 1 fimbrial adhesin gene, and recent molecular studies have demonstrated that this lineage emerged in the early 2000s and was then followed by the rapid expansion of its sublineages H30-R and H30-Rx. K. pneumoniae ST258 comprises 2 distinct lineages, namely clade I and clade II. Moreover, it seems that ST258 is a hybrid clone that was created by a large recombination event between ST11 and ST442. Epidemic plasmids with bla CTX-M and bla KPC belonging to incompatibility group F have contributed significantly to the success of these clones. E. coli ST131 and K. pneumoniae ST258 are the quintessential examples of international multidrug-resistant high-risk clones.

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Section: Initial Studies Pertaining To K Pneumoniae St258mentioning

confidence: 99%

Section: Initial Studies Pertaining To K Pneumoniae St258mentioning

confidence: 99%

Section: Recent Developments Pertaining To K Pneumoniae St258mentioning

confidence: 99%

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The Role of Epidemic Resistance Plasmids and International High-Risk Clones in the Spread of Multidrug-Resistant Enterobacteriaceae

2015

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“…The Carba NP test has been extensively validated for the detection of carbapenemase producers in Enterobacteriaceae and Pseudomonas spp. (13)(14)(15)(16)(17)(18)(19)(20), and a slightly modified version of this test (CarbAcineto NP) has been developed for the detection of carbapenemase-producing Acinetobacter spp. (21).…”

mentioning

confidence: 99%

Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers

2015

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bPerformances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory worldwide. Multidrug resistance in Gram-negative bacteria is growing at an alarming rate (1). In this context, carbapenemase producers are becoming extremely important (2, 3). Rapid detection of carbapenemase producers provides critical information for antibiotic stewardship and rapid implementation of outbreak control measures (3).Few techniques are available for the rapid identification of carbapenemase producers (4) and include UV spectrophotometry (5), matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) technology (6-8), and molecular techniques (9-12). These techniques have overall good sensitivities and specificities but may require trained microbiologists, expensive equipment, and may be time-consuming and expensive. In addition, molecular techniques may fail to detect unknown carbapenemase genes or genes not included in the given gene panel.A rapid and biochemical detection of carbapenemase production was recently proposed, namely, the Carba NP test, which is based on the detection of the hydrolysis of the ␤-lactam ring of imipenem (13). The Carba NP test has been extensively validated for the detection of carbapenemase producers in Enterobacteriaceae and Pseudomonas spp. (13)(14)(15)(16)(17)(18)(19)(20), and a slightly modified version of this test (CarbAcineto NP) has been developed for the detection of carbapenemase-producing Acinetobacter spp. (21). Here, we evaluated the performance of the Rapidec Carba NP test (bioMérieux, La Balme-les-Grottes, France) from bacterial cultures and compared its performance to those of the Carba NP test (13) and the CarbAcineto NP test (21).Performance of the Rapidec Carba NP test compared to that of the Carba NP test. A total of 176 strains were used to evaluate the performance of the Rapidec Carba NP test. They were from various clinical origins (blood culture, urine, sputum, gut flora), of worldwide origin, and isolated from 2010 to 2014. These strains had previously been characterized for their carbapenemase content at the molecular level (Table 1). This strain collection included the most frequently acquired carbapenemases identified worldwide, including those found in Enterobacteriaceae, Pseudomonas sp., and Acinetobacter sp. clinical isolates, and also rare carbapenemases. Susceptibility testing was performed by determining MIC values using the Etest (bioMérieux) on Mueller-Hinton agar plates at 37°C, and results were recorded according to U.S. guidelines (CLSI) as updated in 2014 (22). A total of 98 isolates producing all types of carbapenem-hydrolyzing ␤-lactamases were included, with 75 carbapenemase-negative strains either carbapenem susceptible (...

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“…Asimismo, varios estudios de vigilancia llevados a cabo en Israel demostraron que la prevalencia del clon ST258 sigue en aumento, pues pasó de 65 % en el 2008 hasta el 80 % en el 2013 (32).…”

Section: Discussionunclassified

Caracterización molecular de un brote de Klebsiella pneumoniae resistente a carbapenémicos en un hospital de alto nivel de complejidad de Medellín, Colombia

et al. 2015

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Persistence of Klebsiella pneumoniae ST258 as the predominant clone of carbapenemase-producing Enterobacteriaceae in post-acute-care hospitals in Israel, 2008-13

Abstract: Despite the decreased prevalence of CPE in Israeli PACHs, and the emergence of new types of CPE, the KPC-KP ST258 clone remains the predominant clone represented.

Cited by 58 publications

References 22 publications

The Role of Epidemic Resistance Plasmids and International High-Risk Clones in the Spread of Multidrug-Resistant Enterobacteriaceae

The Role of Epidemic Resistance Plasmids and International High-Risk Clones in the Spread of Multidrug-Resistant Enterobacteriaceae

Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers

Caracterización molecular de un brote de Klebsiella pneumoniae resistente a carbapenémicos en un hospital de alto nivel de complejidad de Medellín, Colombia

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