Persistence of Neuroadapted Mumps Virus in Brains of Newborn Hamsters after Intraperitoneal Inoculation

Abstract

Neuroadapted mumps virus produces systemic infection in newborn hamsters after intraperitoneal inoculation. Virus is disseminated via a low-level viremia and appears to enter the central nervous system by passage through the choroid plexus. At such sites, choroidal and ependymal epithelial cells are productively infected and become a source for further viral spread throughout the brain parenchyma. The development of neutralizing and hemagglutination-inhibiting antibodies in serum correlates with the clearance of virus from most systemic sites. However, peristence of virus in both brain and kidney is demonstrated late in this infection.

The F Gene of Rodent Brain-Adapted Mumps Virus Is a Major Determinant of Neurovirulence

“…Cells were infected/transfected, fixed, processed, and mounted as previously described (16). A primary anti-MuV F monoclonal antibody (5.418) was used at a dilution of 1:500 (30 50 ) of the viruses in the right parietal area of the skull, approximately 1 mm right of the midline and midway between the eye and the ear under mild isoflurane anesthesia (10 l). Fourteen to 50 animals per virus were infected.…”

“…Encephalitis in the brains of newborn hamsters infected with rodent brainadapted strains of MuV has been studied extensively (21). Both intracerebral inoculation and intraperitoneal inoculation with the MuV KH strain lead to widespread CNS infection and mortality (21,50). However, the hamster model is unable to discriminate between MuV strains with known differences in human neurovirulence; comparison of the neurovirulence of three strains of MuV revealed that the nonneurovirulent Jeryl Lynn vaccine was most similar to the rodent brain-adapted MuV KH strain (51).…”

Studies on the Association of Meningitis and Mumps Virus Vaccination

“…Mumps virus can also infect urinary tract, genital organs, pancreas, kidney and central nervous system (CNS). It is not yet wellknown how mumps virus spreads to the CNS, however, studies in newborn hamster model suggest that virus spreads by passage of infected mononuclear cells across the epithelium to epithelial cells of the choroid plexus (Fleischer & Kreth, 1982;Wolinsky et al, 1976). Alternatively, direct spread of virus is possible.…”

Tioman Virus, a Paramyxovirus of Bat Origin, Causes Mild Disease in Pigs and Has a Predilection for Lymphoid Tissues

“…1K). Circulating leukocytes in viral dissemination have been reported in mumps and measles virus infections (13,19).…”