Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types

Frémaux, B.; Prigent‐Combaret, Claire; Delignette-Muller, Marie Laure; Mallen, Benoît; Dothal, M.; Gleizal, A.; Vernozy-Rozand, C.

doi:10.1111/j.1365-2672.2007.03532.x

Cited by 21 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Four strains of serotype 026:F111 were inoculated in manure-emended loam and clay loam soils at a level of approximately 6 log CFU/g. At 20°C, survival of the 026:H11 strains in emended soil ranged from 196 to >365 days, and at 4°C, the survival was 288 to >365 days (20). demonstrated that non-0157 STEC had prolonged survival in cattle waste such as manure or slurry and that non-0157 STEC survived for long periods in soil containing cow manure.…”

Section: Survival Of Stec In Cattle Manure and Soilmentioning

confidence: 95%

See 1 more Smart Citation

Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli

Smith

Fratamico

2012

Journal of Food Protection

View full text Add to dashboard Cite

Non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC) strains have emerged as important foodborne pathogens worldwide. Non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145 have been declared as adulterants in beef by the U. S. Department of Agriculture Food Safety and Inspection Service. While documentation is limited, treatments including heat and acid that have been shown to inactivate E. coli O157:H7 will likely also destroy non-O157 STEC; however, non-O157 STEC strains show variability in their responses to stress. It has been shown that non-O157 STEC may survive in fermented sausages and cheeses, and treatments such as high pressure may be necessary to eliminate non-O157 STEC from these products. The mechanisms used by non-O157 STEC to resist acid environments are similar to those used by O157:H7 strains and include the acid tolerance response, the oxidative system, and the glutamate and arginine decarboxylase systems. However, one study demonstrated that some non-O157 STEC strains utilize a chaperone-based acid stress response (HdeA and HdeB) to combat acidic conditions, which is lacking in E. coli O157:H7. Genomic studies suggest that while non-O157 STEC can cause diseases similar to those caused by E. coli O157:H7, O157 and non-O157 STECs have different evolutionary histories. Non-O157 STECs are a heterogeneous group of organisms, and there is currently a limited amount of information on their virulence, fitness, and stress responses, rendering it difficult to draw firm conclusions on their behavior when exposed to stress in the environment, in food, and during processing.

show abstract

Section: Survival Of Stec In Cattle Manure and Soilmentioning

confidence: 95%

“…STEC serotype 026:H11 survived for long periods in soils emended with cow manure (20). Four strains of serotype 026:F111 were inoculated in manure-emended loam and clay loam soils at a level of approximately 6 log CFU/g.…”

Section: Survival Of Stec In Cattle Manure and Soilmentioning

confidence: 99%

Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli

Smith

Fratamico

2012

Journal of Food Protection

View full text Add to dashboard Cite

show abstract

“…Although there are leafy green vegetable associated outbreaks caused by Salmonella and Cyclospora , a majority of them have been due to food contamination with Escherichia coli O157:H7 (Sivapalasingam et al, 2004). The most likely mechanisms of E. coli O157: H7 contaminations include contamination from soil amendments (i.e., manure, compost and compost teas), water (irrigation or flooding/runoff from adjacent land), wildlife, and airborne deposition from off-farm activities such as cattle/dairy and manure/composting operations (Franz et al, 2008, 2011; Fremaux et al, 2008; van Elsas et al, 2011). One of the worst incidents to date was a multistate Escherichia coli O157:H7 outbreak in August and September 2006, which was associated with consumption of fresh, bagged spinach that was traced to a field in California (California Food Emergency Response Team, 2007a,b; Cooley et al, 2007; Jay et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Topological data analysis of Escherichia coli O157:H7 and non-O157 survival in soils

Ibekwe¹,

Ma²,

Crowley³

et al. 2014

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Shiga toxin-producing E. coli O157:H7 and non-O157 have been implicated in many foodborne illnesses caused by the consumption of contaminated fresh produce. However, data on their persistence in soils are limited due to the complexity in datasets generated from different environmental variables and bacterial taxa. There is a continuing need to distinguish the various environmental variables and different bacterial groups to understand the relationships among these factors and the pathogen survival. Using an approach called Topological Data Analysis (TDA); we reconstructed the relationship structure of E. coli O157 and non-O157 survival in 32 soils (16 organic and 16 conventionally managed soils) from California (CA) and Arizona (AZ) with a multi-resolution output. In our study, we took a community approach based on total soil microbiome to study community level survival and examining the network of the community as a whole and the relationship between its topology and biological processes. TDA produces a geometric representation of complex data sets. Network analysis showed that Shiga toxin negative strain E. coli O157:H7 4554 survived significantly longer in comparison to E. coli O157:H7 EDL 933, while the survival time of E. coli O157:NM was comparable to that of E. coli O157:H7 EDL 933 in all of the tested soils. Two non-O157 strains, E. coli O26:H11 and E. coli O103:H2 survived much longer than E. coli O91:H21 and the three strains of E. coli O157. We show that there are complex interactions between E. coli strain survival, microbial community structures, and soil parameters.

show abstract

“…In addition to temperature, the indigenous microflora (93), scarcity of nutrients (96), and protozoan predations also influence survival. Recent passage of VTEC through the bovine gastrointestinal tract may enhance survival of O157 in water (97).…”

Section: Vtec In Watermentioning

confidence: 99%

“…However, it should be noted that not all strains belonging to these serogroups will cause severe illness and that other non-O157 VTEC serogroups also cause illness. Pathogenic VTEC strains are categorized as enterohemorrhagic E. coli, and usually in these strains, a large outer membrane protein (94)(95)(96)(97) called intimin mediates the intimate contact between the bacterium and the enterocyte cytoplasmic membrane (attachment) and the destruction of the enterocyte microvilli (effacement). The genetic determinants for this (eae, tir, esc, and sep genes) are grouped together on the chromosome, forming a pathogenicity island called LEE, for locus of enterocyte effacement (7).…”

Section: Human Pathogenic Vtecmentioning

confidence: 99%

Veterinary Public Health Approach to Managing Pathogenic Verocytotoxigenic Escherichia coli in the Agri-Food Chain

Duffy

McCabe

2014

Microbiol Spectr

View full text Add to dashboard Cite

Verocytoxigenic Escherichia coli (VTEC) comprises many diverse serogroups, but seven serogroups, O157, O26, O103, O145, O111, O21, and O45, have been most commonly linked to severe human infections, though illness has also been reported from a range of other VTEC serogroups. This poses challenges in assessing the risk to humans from the diverse range of VTEC strains that may be recovered from animals, the environment, or food. For routine assessment of risk posed by VTEC recovered from the agri-food chain, the concept of seropathotype can be used to rank the human risk potential from a particular VTEC serogroup on the basis of both serotype (top seven serogroups) and the presence of particular virulence genes (vt in combination with eae, or aaiC plus aggR). But for other VTEC serogroups or virulence gene combinations, it is not currently possible to fully assess the risk posed. VTEC is shed in animal feces and can persist in the farm environment for extended periods ranging from several weeks to many months, posing an ongoing reservoir of contamination for grazing animals, water courses, and fresh produce and for people using farmland for recreational purposes. Appropriate handling and treatment of stored animal waste (slurries and manures) will reduce risk from VTEC in the farm environment. Foods of animal origin such as milk and dairy products and meat may be contaminated with VTEC during production and processing, and the pathogen may survive or grow during processing operations, highlighting the need for well-designed and validated Hazard Analysis Critical Control Point management systems. This article focuses on a veterinary public health approach to managing VTEC, highlighting the various routes in the agri-food chain for transmission of human pathogenic VTEC and general approaches to managing the risk.

show abstract

Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types

Abstract: This study provides useful information regarding the ecology of STEC O26 in manure-amended soils and may have implications for land and waste management.

Cited by 21 publications

References 42 publications

Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli

Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli

Topological data analysis of Escherichia coli O157:H7 and non-O157 survival in soils

Veterinary Public Health Approach to Managing Pathogenic Verocytotoxigenic Escherichia coli in the Agri-Food Chain

Contact Info

Product

Resources

About