Persistence of transgenic genes and proteins during soybean food processing

González-Morales, Susana; Cruz‐Requena, Marisol; Rodríguez-Vidal, Arturo; Aguilar, Cristóbal N.; Rebolloso-Padilla, Óscar Noé; Rodríguez‐Herrera, Raúl

doi:10.1016/j.fbio.2015.04.002

Cited by 2 publications

(1 citation statement)

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“…Many countries established legislation for genetically modified organism (GMO) labelling of grains, feed and foodstuffs to provide consumer information. Generally labelling is mandatory if GMO content exceeds a certain threshold level (Gonzalez-Morales et al, 2015;Kamle & Ali, 2013;Lin & Pan, 2016). European Union (EC 1829(EC /2003 and Russia established food labelling above 0.9% GMO, Australia, Brazil, New Zealand, Saudi Arabia and Israel 1%, Chile 2% and Korea 3%.…”

Section: Introductionmentioning

confidence: 99%

New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR

et al. 2018

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The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization.

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Section: Introductionmentioning

confidence: 99%