Persistence of uterine bacterial infection, and its associations with endometritis and ovarian function in postpartum dairy cows

Ghanem, Mohamed Elshabrawy; Tezuka, Erisa; Devkota, Bhimsen; Izaike, Yoshiaki; Osawa, Takeshi

doi:10.1262/jrd.2014-051

Cited by 34 publications

(38 citation statements)

References 49 publications

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“…Eleven out of 17 (64Á7%) uterine samples had polymorphonuclear neutrophils (PMNs), more than 3% of which confirmed the presence of endometritis in these cows. A low (Bara nski et al 2012) to moderate (Ghanem et al 2015) correlation between the uterine cytology and bacteriology has been previously reported in postpartum endometritic dairy cows. Out of seven isolates of Staphylococcus aureus from the uterine samples, 6 (85%) were coagulase positive.…”

Section: Resultsmentioning

confidence: 91%

Detection of bacteria in bovine ovarian follicular fluid

Salary

Kafi

Derakhshandeh

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: The results of the present study show that ovarian follicular fluid is not sterile in bovines. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows. AbstractThe aim of this study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. In all, 17 samples of paired follicular fluids and uterine contents collected from post-slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Furthermore, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also investigated using PFGE analysis. Follicular fluid concentrations of lipopolysaccharides were also determined. Of 17 uterine samples, 15 (88%) were detected as contaminated. In total, nine different bacterial genera and species were identified in the uterine and follicular fluid samples. Escherichia coli was the most prevalent bacterial species isolated from the uterine samples. Out of seven isolates of Staphylococcus aureus from the uterine samples, 6 (85%) were coagulase positive. Six isolates of Staphylococcus spp. were identified in 6 out of 17 follicular fluid samples (35%). Two out of six isolates were identified as Staphylococcus aureus (33%). Our results show that ovarian follicular fluid is not sterile in the bovine. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows.

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Section: Resultsmentioning

confidence: 91%

Detection of bacteria in bovine ovarian follicular fluid

Salary

Kafi

Derakhshandeh

et al. 2020

Lett Appl Microbiol

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“…Some biological markers such as CD4 +, CD14 + and CD25 + leukocyte cells were in lesser concentrations in cows with SCE in peripheral blood. CD21 + and CD8 + lymphocyte cells were significantly decreased in SCE cows from uterine flushes (Ghanem et al, 2015).…”

Section: Immune Responsementioning

confidence: 88%

“…The process through which bacteria exerts its effects is still uncertain. It is clear that the presence of bacteria is related to clinical and/or subclinical uterine inflammation (Ghanem et al, 2015). The presence of specific bacterial populations in the uterus is also a factor involved in uterine disease.…”

Section: Infectious Factorsmentioning

confidence: 99%

“…The presence of specific bacterial populations in the uterus is also a factor involved in uterine disease. Cows with Trueperella pyogenes had a greater risk of presenting PVD, purulent uterine lavage, SCE and poor reproductive performance (Prunner et al, 2014a;Ghanem et al, 2015;Wagener et al, 2015;Bicalho et al, 2016). It is known that T. pyogenes secrete an exotoxin called pyolysin.…”

Section: Infectious Factorsmentioning

confidence: 99%

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Uterine inflammation affects the reproductive performance of dairy cows: A review

Molina‐Coto

Lucy

2018

Mesoam. Agron.

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Incidence of post-partum uterine disease is an important concern in dairy cattle, because it affects its reproduction. Therefore, the objective of this review of literature was to generate a multifactorial overview about uterine diseases, and the reproductive performance of dairy cows, from a zootechnical approach. Dairy cows face multiple challenges around parturition. Immune suppression around calving, exposition to trauma and uterine bacterial contamination, metabolic diseases, lactation, and changes in management make dairy cows susceptible to uterine diseases. Most cows are able to eliminate uterine infection after calving, however, some cows keep uterine disease. Uterine disease may show clinical signs, but also silent signs that affect fertility as well. Poor reproductive performance is not caused by those signs by themselves, but due to alterations in ovarian and uterine function. Also, the problem of this silent signs is that farmers become aware of the disease when it has already caused negative effects on the reproductive performance. Sometimes, uterine disease is still present at the moment of the first service after calving. Uterine disease make it harder for cows to get pregnant because it affects the establishment and maintenance of pregnancy, being another cause for infertility, increasing the cull rate and decreasing incomes from the dairy industry. ResumenEl ganado lechero, entre otros desafíos, presenta enfermedades uterinas post parto que afectan su reproducción, por tanto, el objetivo de esta revisión de literatura fue generar una visión desde múltiples aristas acerca de las enfermedades uterinas y el desempeño reproductivo de la vaca lechera desde un enfoque zootécnico. La vaca lechera enfrenta múltiples retos en el momento del parto. Inmunosupresión alrededor del parto, exposición a trauma y contaminación uterina, enfermedades metabólicas, lactancia y cambios en el manejo, hacen a la vaca lechera un animal vulnerable de sufrir algún tipo de enfermedad uterina. La mayoría de las vacas pueden eliminar la infección Literature review Agron. Mesoam. 29(2):449-468, mayo-agosto, 2018

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“…In a previous study, Ghanem et al [23] reported that while some cows presented with cytological endometritis without clinical endometritis, some others presented with clinical endometritis without cytological endometritis. The assumption that purulent vaginal discharge is associated with endometrial inflammation has never been validated [20].…”

Section: Discussionmentioning

confidence: 99%

Correlation of blood metabolite concentrations and body condition scores with persistent postpartum uterine bacterial infection in dairy cows

Ghanem

Tezuka

Sasaki

et al. 2016

Journal of Reproduction and Development

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To analyze the relationship of blood metabolite concentrations and body condition score (BCS) with persistent bacterial uterine infection, specifically that caused by Trueperella pyogenes and anaerobic bacteria, uterine bacteriological swabs (n = 128) were collected from 64 Holstein cows at 5 (W5) and 7 (W7) weeks postpartum, and the percentage of neutrophils in the endometrium was evaluated. Blood glucose, total cholesterol (T-cho), blood urea nitrogen (BUN), non-esterified fatty acid (NEFA), and β-hydroxybutyric acid concentrations were analyzed at 3 weeks (W-3) and 1 week (W-1) prepartum and W3, W5, and W7 postpartum. BCS were evaluated at W-3, W3, and W7. Blood glucose concentrations at W-3 and W-1 in cows with persistent bacterial infection were lower (P = 0.05) than in the rest of the cows. Total BUN concentrations in cows with persistent bacterial infection were lower (P < 0.01) than those in other cows, although the association between the pre or postpartum time and status of infection was not significant. Total NEFA concentrations in cows with persistent bacterial infection were similar to those in uninfected cows and cows positive for infection at W5 but not W7. Total BCS in cows with persistent bacterial infection were lower (P < 0.01) than those in cows positive for infection at both W5 but not W7 and W7 but not W5; however, the association between the pre or postpartum time and status of infection was not significant. Glucose concentrations at W-3 and W-1 negatively correlated with persistent bacterial infection at W5 and W7 (P < 0.01). BUN concentrations at W3 (P < 0.01), W5 (P < 0.05), and W7 (P < 0.05) and BCS at W3 (P < 0.01) negatively correlated with persistent postpartum bacterial infection. Decreased prepartum blood glucose concentrations might be an important risk factor for persistent postpartum bacterial uterine infection in dairy cows.

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Persistence of uterine bacterial infection, and its associations with endometritis and ovarian function in postpartum dairy cows

Cited by 34 publications

References 49 publications

Detection of bacteria in bovine ovarian follicular fluid

Detection of bacteria in bovine ovarian follicular fluid

Uterine inflammation affects the reproductive performance of dairy cows: A review

Correlation of blood metabolite concentrations and body condition scores with persistent postpartum uterine bacterial infection in dairy cows

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