2016
DOI: 10.3389/fmicb.2016.01485
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Persistent Infection by Wolbachia wAlbB Has No Effect on Composition of the Gut Microbiota in Adult Female Anopheles stephensi

Abstract: The bacteria in the midgut of Anopheles stephensi adult females from laboratory colonies were studied by sequencing the V4 region of 16S rRNA genes, with respect to three experimental factors: stable or cured Wolbachia infection; sugar or blood diet; and age. Proteobacteria and Bacteroidetes dominated the community [>90% of operational taxonomic units (OTUs)]; most taxa were in the classes Flavobacteriia, Gammaproteobacteria, and Alphaproteobacteria, and were assigned to Elizabethkingia (46.9%), Asaia (6.4%) a… Show more

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Cited by 31 publications
(44 citation statements)
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“…aegypti , regardless of Wolbachia infection status. This low level of microbial diversity is consistent with previous studies of laboratory-reared mosquitoes profiled via both culture-dependent and culture-independent methods [23,27,3739]. While the microbiome composition of mosquitoes is known to differ across environments [31], our detection of Flavobacteriaceae/Elizabethkingia and Enterobacteriaceae as a substantial component of the microbiome is in keeping with several other studies of laboratory-reared Aedes and Anopheles species [23,27,29,39,40].…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…aegypti , regardless of Wolbachia infection status. This low level of microbial diversity is consistent with previous studies of laboratory-reared mosquitoes profiled via both culture-dependent and culture-independent methods [23,27,3739]. While the microbiome composition of mosquitoes is known to differ across environments [31], our detection of Flavobacteriaceae/Elizabethkingia and Enterobacteriaceae as a substantial component of the microbiome is in keeping with several other studies of laboratory-reared Aedes and Anopheles species [23,27,29,39,40].…”
Section: Discussionsupporting
confidence: 91%
“…This low level of microbial diversity is consistent with previous studies of laboratory-reared mosquitoes profiled via both culture-dependent and culture-independent methods [23,27,3739]. While the microbiome composition of mosquitoes is known to differ across environments [31], our detection of Flavobacteriaceae/Elizabethkingia and Enterobacteriaceae as a substantial component of the microbiome is in keeping with several other studies of laboratory-reared Aedes and Anopheles species [23,27,29,39,40]. Other genera detected in our study ( Pseudomonas , Serratia , Chryseobacterium , Leucobacter , Staphylococcus , Weeksella ) have also been reported in previous mosquito microbiome studies [23,38,41,42].…”
Section: Discussionsupporting
confidence: 91%
“…Permutational multivariate analysis of variance (PERMANOVA) was used to determine whether microbiota assemblage varied among mosquito developmental stages, different larval habitat types and sampling years (Chen et al., ). PERMANOVA tests (999 mutations) were performed using adonis functions in the vegan package in R 3.2.3.…”
Section: Methodsmentioning
confidence: 99%
“…Rarefaction curves were constructed to determine sample coverage. Based on the OTU picker data, richness estimators (Chao1) and diversity indices (Shannon) were calculated for each mosquito or water sample Chen et al, 2016)…”
mentioning
confidence: 99%
“…600 ng iron per ul after a blood meal (18). The community structure of the microbiome in the mosquito gut varies temporally with episodes of blood and sugar feeding (3, 19). Our specific interest in E. anophelis focuses on understanding the survival mechanisms and physiological adaptations as it establishes and maintains infection in the mosquito gut, where environmental conditions such as iron flux are highly variable.…”
Section: Introductionmentioning
confidence: 99%