Persistent Infection of Human Fibroblasts by Hepatitis a Virus

Vallbracht, Angelika; Hofmann, Lawrence V.; Wurster, Karl; Flehmig, Bertram

doi:10.1099/0022-1317-65-3-609

Cited by 101 publications

(63 citation statements)

References 19 publications

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“…IFN was present during 20 days of culturing) did not reduce production of viral antigen or infectious virus. This is in contrast to the recent work of Vallbracht et al (1984) where it was convincingly shown that exogenous IFN can cure persistently hepatitis A virus-infected cells in vitro. This discrepancy might be explained by differences in the mechanisms by which these viruses establish persistent infection.…”

Section: Discussioncontrasting

confidence: 53%

Influence of Interferon on Persistent Infection Caused by Borna Disease Virus in vitro

Rheinbaben¹,

Stitz²,

Rott³

1985

Journal of General Virology

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SUMMARYThe effect of interferon (IFN) on infection and maintenance of persistent infection of Borna disease (BD) virus in cell cultures was investigated. Acutely BD virus-infected primary rabbit brain and rat lung cells produced significant levels of interferon detectable 3 days post-infection in the culture supernatants. Rat brain and rat lung cells persistently infected with BD virus produced only moderate levels of IFN over a long period. In contrast, persistently infected Madin-Darby canine kidney (MDCK) cells did not produce detectable amounts of IFN. Exogenous homologous IFN completely inhibited the expression of BD virus antigen in acutely infected rabbit brain cells, when added during the first 24 h after infection. IFN added later (2 to 6 days post-infection) reduced virus titres to different degrees depending on the onset of treatment. However, IFN added to persistently infected rat lung cells did not appear to influence the degree or quality of BD virus antigen expression or the intracellular amount of infectious virus. Two facts indicate that IFN is not involved in the establishment or maintenance of persistent BD virus infection in ritro. Thus, MDCK cells, which could not be induced to produce IFN, can be readily persistently infected with BD virus in vitro, and exogenous IFN did not appear to influence persistent BD virus infection.

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Section: Discussioncontrasting

confidence: 53%

Influence of Interferon on Persistent Infection Caused by Borna Disease Virus in vitro

Rheinbaben¹,

Stitz²,

Rott³

1985

Journal of General Virology

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“…This finding is in agreement with Sainokami et al [2005] who found that ALT levels and virus load were significantly correlated in patients with mild HAV-related symptoms, but not in those with severe symptoms. In contrast to the position in vivo, wild-type HAV can persistently infect human cell lines without causing observable cytopathogenicity [Vallbracht et al, 1984]. Cytotoxic peripheral blood lymphocytes taken from HAV patients have been shown to induce cytolysis in HAV-infected cell cultures, whereas lymphocytes from noninfected persons did not have this effect [Vallbracht et al, 1986;Maier et al, 1988].…”

Section: Discussionmentioning

confidence: 99%

High and persistent excretion of hepatitis A virus in immunocompetent patients

et al. 2006

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The duration and level of virus excretion in blood and faeces of patients with hepatitis A virus (HAV) infection were studied in relation to levels of alanine aminotransferase (ALT), disease severity and HAV genotype. Clinical data, blood and faeces were collected from 27 patients with acute hepatitis A (median age: 33 years) for a maximum of 26 weeks. Single blood donations from 55 other patients with acute HAV (median age: 32 years) were also used. Virus loads were quantified by competitive nested RT-PCR. HAV was excreted in faeces for a median period of 81 days after disease onset, with 50% of patients still excreting high levels at Day 36 (2 Â 10 6 -2 Â 10 8 copies/ml faeces suspension). Viraemia was detected, but not quantifiable, for a median period of 42 days. In the first 10 days of illness, higher ALT levels were correlated with higher viraemia levels. Comparison of patients infected with genotype 1a with those infected with type 1b did not differ significantly in terms of the duration of HAV excretion or jaundice. In conclusion, faecal excretion of HAV is at a high titre in the first month, perhaps making patients infectious for a longer period than assumed currently. Blood banks should be aware that viraemia may be present for more than 1 month, and genotype did not affect the duration of virus excretion or jaundice.

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“…Conditions for the growth of hepatitis A virus (HAV) in various cell lines and under various conditions have been described in numerous reports (Provost & Hilleman, 1979;Flehmig, 1980Flehmig, , 1981Flehmig et al, 1981 ;Daemer et al, 1981 ;Gauss-M~ller et al, 1981 ;Kojima et al, 1981 ;Pana et al, 1982;Widell et al, 1984;Binn et al, 1984;Bradley et al, 1984;Vallbracht et al, 1984;Simmonds et al, 1985;Wheeler et al, 1986 a). After initial adaptation of the virus to the appropriate cell line, the growth of HAV in cell culture has been reported to be non-cytopathic and usually results in a persistent infection.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to the extended periods needed for initial cell adaptation of virus from clinical specimens (Fr6sner et al, 1979;Daemer et al, 1981 ;Gauss-Mfiller et al, 1981 ;Binn et al, 1984;Bradley et al, 1984), cell culture-adapted virus becomes detectable by immunoassay or immunofluorescence within 1 to 17 days (Vallbracht et al, 1985;Simmonds et al, 1985;Wheeler et al, 1986 a;Cromeans et al, 1987). Although persistent infection has been reported to be the most practical method to grow HAV (Vallbracht et al, 1984;Simmonds et al, 1985;Wheeler et al, 1986b), our findings indicate that over time the amount of virus produced by persistently infected FRhK4 cells decreased significantly. One explanation for this was that over a long period, the cells more sensitive to HAV growth were destroyed by infection, and a population of cells resistant to HAV infection was selected.…”

Section: Effect Of Serum Concentration and Growth Time On Ha V-ag Promentioning

confidence: 99%

Large Scale Production of Hepatitis A Virus in Cell Culture: Effect of Type of Infection on Virus Yield and Cell Integrity

Robertson

Khanna²,

Brown³

et al. 1988

Journal of General Virology

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SUMMARYApproaches to cell culture propagation of hepatitis A virus (HAV) have used either acute infection by passage of infected cell lysates or supernatants into uninfected cells or the passage of persistently infected cells. The findings presented here demonstrate that the growth and recovery of purified virus from foetal rhesus monkey kidney (FRhK4) cells persistently infected with HAV isolate HAS-15 decreased over a 2 to 3 month period. In contrast, high multiplicity acute infection of FRhK4 cells with purified HAS-15 HAV resulted in degeneration of the cell monolayer 2 to 3 weeks later. Large scale propagation of acutely infected cells followed by traditional picornavirus purification procedures reproducibly yielded milligram amounts of purified virus.

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Persistent Infection of Human Fibroblasts by Hepatitis a Virus

Cited by 101 publications

References 19 publications

Influence of Interferon on Persistent Infection Caused by Borna Disease Virus in vitro

Influence of Interferon on Persistent Infection Caused by Borna Disease Virus in vitro

High and persistent excretion of hepatitis A virus in immunocompetent patients

Large Scale Production of Hepatitis A Virus in Cell Culture: Effect of Type of Infection on Virus Yield and Cell Integrity

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