Persistent Listeria monocytogenes strains isolated from mussel production facilities form more biofilm but are not linked to specific genetic markers

Nowak, Jessika; Cruz, Cristina D.; Tempelaars, Marcel H.; Abee, Tjakko; Vliet, Arnoud H. M. van; Fletcher, Graham C.; Hedderley, Duncan; Palmer, John W.; Flint, Steve

doi:10.1016/j.ijfoodmicro.2017.05.024

Cited by 53 publications

(41 citation statements)

References 51 publications

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“…A particular risk group are catheterised patients. Due to its ability to produce biofilm, L. monocytogenes is more resistant to antibiotics, host immune system [7,8], biocides, or stress [13,14]. L. monocytogenes can produce biofilms on various surfaces used in medicine or industry, such as polystyrene, glass, and stainless steel [15,16].…”

Section: Discussionmentioning

confidence: 99%

Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes

Skowron

Brożek

Łukasik

et al. 2020

Disaster Emerg Med J

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INTRoduCTIoN: Listeria monocytogenes is a cause of listeriosis, which is dangerous especially for the elderly, immunocompromised people, and pregnant women. The ability of these pathogens to colonise biotic and abiotic surfaces and form biofilm poses a serious threat for hospitalised, catheterised patients. MATERIAL ANd METhodS: The study was conducted on 29 L. monocytogenes strains isolated from clinical materials (blood, cerebrospinal fluid, swabs from vagina) and the reference strain L. monocytogenes ATCC 1911. The ability of the tested strains to form biofilm in 96-well plates and their drug susceptibility (disk diffusion method) was determined. RESuLTS: All strains formed biofilm, but its intensity was correlated with the source of isolation. A strong biofilm formed in 72.73% of isolates from cerebrospinal fluid [(A570 0.421-1.3), 75.0 % of blood isolates 9 (A570 0.389-1.063), and 50.0 % of isolates from vaginal swabs (A570 0.457-0.487)]. The strongest biofilm was formed by strains derived from cerebrospinal fluid whereas isolates from vaginal swabs, which strongly formed a biofilm, accounted for 50.0% of the studied population (absorbance 0.457-0.487). It was found that 93.1% (n = 27) of strains were susceptible to all drugs tested. Two strains (6.9%) were resistant to cotrimoxazole and one strain (3.45 %) to erythromycin. CoNCLuSIoNS: The diverse ability of clinical L. monocytogenes strains to form biofilm is an important aspect in the prophylaxis of catheterised patients.

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Section: Discussionmentioning

confidence: 99%

Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes

Skowron

Brożek

Łukasik

et al. 2020

Disaster Emerg Med J

View full text Add to dashboard Cite

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“…) with some studies suggesting that persistent strains form better biofilms than sporadic strains (Nowak et al . ) whereas others show no difference in biofilm formation between persistent and sporadic strains (Magalhães et al . ).…”

Section: Potential Survival Mechanisms Of L Monocytogenes In the Foomentioning

confidence: 98%

“…Potential survival mechanisms of L. monocytogenes in the food processing environment Persistent strains have been shown to exist in the processing environment, but studies which have tried to explain the physiological characteristics which contribute to L. monocytogenes persistence vary in their findings. For example, it has been reported that persistent strains show enhanced adherence to food contact surfaces after short contact times (Lund en et al 2000) with some studies suggesting that persistent strains form better biofilms than sporadic strains (Nowak et al 2017) whereas others show no difference in biofilm formation between persistent and sporadic strains (Magalhães et al 2017). Persistent strains have also been shown to be more resistant to detergent stresses, although this attribute may be due to the characteristics of biofilms rather than intrinsic resistance of the bacterial cell (Pan et al 2006).…”

Section: Harbourage Sites and Persistent Strains In Food Processing Fmentioning

confidence: 99%

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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Listeria monocytogenes is an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processed on a large scale before reaching the consumer through various products such as sandwiches and prepared salads. From farm to fork, the fresh leafy produce supply chain (FLPSC) is complex and contains a diverse range of environments where L. monocytogenes is sporadically detected during routine sampling of produce and processing areas. This review describes sources of the bacteria in the FLPSC and outlines the physiological and molecular mechanisms behind its survival in the different environments associated with growing and processing fresh produce. Finally, current methods of source tracking the bacteria in the context of the food supply chain are discussed with emphasis on how these methods can provide additional, valuable information on the risk that L. monocytogenes isolates pose to the consumer.

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“…While all L. monocytogenes in this study formed biofilm, some formed significantly more biofilm than others on polystyrene 96-well plates when grown in MWB, a minimally defined media. This result suggests that some strains may have a competitive advantage over others in the fresh produce supply chain based on their ability to form biofilm, but previous research has generated mixed results when determining whether strong biofilm formation is an indicator of persistence in processing environments (Magalhães et al, 2017;Nowak et al, 2017). Furthermore, it is recognised that many environmental factors contribute to biofilm production in L. monocytogenes including substrate, surface material and temperature.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential

Smith

Hearn

Taylor

et al. 2019

International Journal of Food Microbiology

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Listeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh produce and their potential pathogenicity. In this work, fifteen Listeria monocytogenes isolates from the UK fresh produce supply chain were characterised using whole genome sequencing (WGS). Additionally, isolates were characterised based on their ability to form biofilm. Whole genome sequencing data was used to determine the sequence type of isolates based on multi-locus sequence typing (MLST), construct a core single nucleotide polymorphism (SNP) phylogeny and determine the presence of virulence and resistance associated genes. MLST revealed 9 distinct sequence types (STs) spanning 2 lineages (I & II) with one isolate belonging to the ST6 subtype, strains from which have been recently implicated in two large, food-associated L. monocytogenes outbreaks in South Africa and across Europe. Although most of the 15 isolates were different, comparison of core genome SNPs showed 4 pairs of 'indistinguishable' strains (< 5 SNPs difference). Virulence profiling revealed that some isolates completely lacked the Listeria pathogenicity island-3 (LIPI-3) amongst other virulence factors. Investigation of the inlA gene showed that no strains in this study contained a premature stop codon (PMSC), an indicator of ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T2 attenuated virulence. Assessment of biofilm production showed that isolates found in the fresh produce supply chain differ in their ability to form biofilm. This trait is considered important for L.monocytogenes to persist in environments associated with food production and processing. Overall the work indicates that a genetically diverse range of L. monocytogenes strains is present in the UK fresh produce supply chain and the virulence profiles found suggests that at least some of the strains are capable of causing human illness. Interestingly, the presence of some genetically indistinguishable isolates within the 15 isolates examined suggests that cross-contamination in the fresh produce environment does occur. These findings have useful implications in terms of food safety and for informing microbial surveillance programmes in the UK fresh produce supply chain.

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Persistent Listeria monocytogenes strains isolated from mussel production facilities form more biofilm but are not linked to specific genetic markers

Cited by 53 publications

References 51 publications

Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes

Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential

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