Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates

Novikova, Liudmila N.; Novikov, Lev N.; Kellerth, Jan‐Olof

doi:10.1016/s0165-0270(97)02227-9

Cited by 154 publications

(160 citation statements)

References 28 publications

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“…Less labelling was reported in rat motoneurones retrogradely labelled with FG at four weeks compared to one week after tracer application to a peripheral nerve, whereas the number of FB neurones appeared to remain unchanged up to 24 weeks (Novikova et al, 1997).…”

Section: Detection Of Labelled Neurones In the Drgmentioning

confidence: 83%

“…Garrett et al (1991) suggested a toxic effect of FG resulting in cell death at long survival times. The finding by Novikova et al (1997), however, that the number of FB-labelled motoneurones stays unchanged for long periods of time after combined FB and FG administration argues against toxicity as a general problem.…”

Section: Double Labellingmentioning

confidence: 97%

“…For FG, concentrations ranging from 2 to 5% have been used (Schmued and Fallon, 1986;Harsh et al, 1991;Novikova et al, 1997;Madison et al, 1996). We used a concentration of 2% FG in the double labelling experiments, as preliminary experiments showed that the use of this concentration in combination with 5% FB yielded similar mean numbers of labelled neurones.…”

Section: Concentration and Amount Of Tracer Injectedmentioning

confidence: 99%

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Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Puigdellı́vol-Sánchez

Prats-Galino

Ruano-Gil

et al. 1998

Journal of Neuroscience Methods

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Section: Detection Of Labelled Neurones In the Drgmentioning

confidence: 83%

Section: Double Labellingmentioning

confidence: 97%

Section: Concentration and Amount Of Tracer Injectedmentioning

confidence: 99%

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Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Puigdellı́vol-Sánchez

Prats-Galino

Ruano-Gil

et al. 1998

Journal of Neuroscience Methods

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“…FB is one of the most frequently used fluorescent agents for retrograde tracing experiments. It is taken up by axon terminals rather than fibers of passage (Kobbert et al 2000) and exerts a cytoplasmic labeling of neurons 1-2 weeks after its injection (Novikova et al 1997). Among all fluorescent tracers, FB is characterized by its high staining effectiveness and good tissue penetration (Choi et al 2002).…”

Section: Methodological Considerationsmentioning

confidence: 99%

Topography and collateralization of dopaminergic projections to primary motor cortex in rats

2015

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Dopaminergic signaling within the primary motor cortex (M1) is necessary for successful motor skill learning. Dopaminergic neurons projecting to M1 are located in the ventral tegmental area (VTA, nucleus A10) of the midbrain. It is unknown which behavioral correlates are encoded by these neurons. The objective here is to investigate whether VTA-M1 fibers are collaterals of projections to prefrontal cortex (PFC) or nucleus accumbens (NAc) or if they form a distinct pathway. In rats, multiple-site retrograde fluorescent tracers were injected into M1, PFC and the core region of the NAc and VTA sections investigated for concomitant labeling of different tracers. Dopaminergic neurons projecting to M1, PFC and NAc were found in nucleus A10 and to a lesser degree in the medial nucleus A9. Neurons show high target specificity, minimal collateral branching to other than their target area and hardly cross the midline. Whereas PFC-and NAc-projecting neurons are indistinguishably intermingled within the ventral portion of dopaminergic nuclei in middle and caudal midbrain, M1-projecting neurons are only located within the dorsal part of the rostral midbrain. Within M1, the forelimb representation receives sevenfold more dopaminergic projections than the hindlimb representation. This strong rostro-caudal gradient as well as the topographical preference to dorsal structures suggest that projections to M1 emerged late in the development of the dopaminergic systems in and form a functionally distinct system.

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“…A variety of retrograde and anterograde fluorescent tracers, such as carbocyanine dyes or dextran amines can be visualized under the microscope and provide numerous approaches for analyzing neuronal circuits [14,15]. Thus, fluorescent markers in combination with techniques such immunohistochemistry or electrophysiological recording provide the opportunity to study various neurobiological questions [14,16]. In contrast to nonfluorescent tracers, fluorescent markers offer the major advantage of the simultaneous examination in vitro and in vivo [8].…”

Section: Discussionmentioning

confidence: 99%

Mini-Ruby is Rapidly Taken up by Neurons and Astrocytes in Organotypic Brain Slices

Ullrich

Humpel

2011

Neurochem Res

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Cholinergic neurons are intensively studied, because they degenerate in Alzheimer's disease. Although neurotracer techniques are widely used to study axonal transport, guidance, regeneration or sprouting it is not clear if cholinergic neurons can be stained by tracer techniques and studied in brain slices. The aim of the present study was to evaluate the characteristics of the neurotracer Miniruby in organotypic brain slices of the basal nucleus of Meynert (nBM), focusing on cholinergic neurons. Miniruby is a biotinylated dextran amine and is taken up very fast by a variety of cells. When 2-week old nerve growth factor-incubated brain slices of the nBM were treated with Mini-ruby crystals for 1 h, only a few (2-3%) cholinergic neurons were clearly labeled as shown by co-localization with choline acetyltransferase. The staining was found in neuN-positive neurons and microtubule associated protein-2 (MAP-2)-positive nerve fibers. A very rapid dynamic change was observed in these labeled varicosities within seconds. However, Mini-ruby was taken up also by many glutamine synthethase-positive astrocytes. At the site of Mini-ruby application an intense CD11b-positive microglial staining was evident. In conclusion, neurons and astrocytes in organotypic brain slices can be labeled very fast with the fluorescent dye Mini-ruby which undergoes dynamic processes.

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Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates

Cited by 154 publications

References 28 publications

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Topography and collateralization of dopaminergic projections to primary motor cortex in rats

Mini-Ruby is Rapidly Taken up by Neurons and Astrocytes in Organotypic Brain Slices

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