Persistent Patterns of E. coli Concentrations in Two Irrigation Ponds from 3 Years of Monitoring

Stocker, Matthew; Pachepsky, Yakov; Smith, Jaclyn E.; Morgan, Billie; Hill, Robert L.; Kim, Moon S.

doi:10.1007/s11270-021-05438-z

Cited by 11 publications

(3 citation statements)

References 41 publications

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“…It is possible that the die-off rates in the pond were slightly larger than the replenishment of E. coli into the pond from the inflow water source. Identification of inflows to agricultural ponds appears important because these can act as a constant source of microorganisms and may also result in localized hot spots of fecal bacteria (Stocker et al, 2021). We cannot rule out the effect of undiscovered inflows in P1 or P2 as a source of E. coli to the ponds, which may have affected the trends observed in the current study.…”

Section: Discussionmentioning

confidence: 80%

Intra‐daily variation of Escherichia coli concentrations in agricultural irrigation ponds

et al. 2022

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Microbial water quality is determined by comparing observed Escherichia coli concentrations with regulatory thresholds. Measured concentrations can be expected to change throughout the course of a day in response to diurnal variation in environmental conditions, such as solar radiation and temperature. Therefore, the time of day at which samples are taken is an important factor within microbial water quality measurements. However, little is known about the diurnal variations of E. coli concentrations in surface sources of irrigation water. The objectives of this work were to evaluate the intra‐daily dynamics of E. coli in three irrigation ponds in Maryland over several years and to determine the water quality parameters to which E. coli populations are most sensitive. Water sampling was conducted across the ponds at 0900, 1200, and 1500 h on a total of 17 dates in the summers of 2019–2021. One‐way ANOVA revealed significant diurnal variability in E. coli concentrations in Pond (P)1 and P2, whereas no significant effects were observed in P3. Escherichia coli die‐off rates calculated between sampling time points in the same day were significantly higher in P2 than in P1 and P3, and these rates ranged from 0.005 to 0.799 h–1 across ponds. Concentrations of dissolved oxygen, pH, conductivity, and turbidity exerted the most control over E. coli populations. Results of this work demonstrate that sampling in the early‐morning hours provides the most conservative assessment of the microbial quality of irrigation waters.

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Section: Discussionmentioning

confidence: 80%

Intra‐daily variation of Escherichia coli concentrations in agricultural irrigation ponds

et al. 2022

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“…The average concentrations of E. coli in the pond water showed correlations with days since rainfall and with rainfall amounts in the previous 72 h. The effect of precipitation on concentrations of fecal indicator bacteria in agricultural ponds has been documented by several researchers, and the positive correlation is most often attributed to runoff containing high concentrations of indicators entering the pond waters [30][31][32][33] and sediment resuspension [34][35][36]. However, poor correlations between indicator or pathogen concen-…”

Section: Discussionmentioning

confidence: 99%

Spatial Variation of Tetracycline-Resistant E. coli and Relationships with Water Quality Variables in Irrigation Water: A Pilot Study

Stocker

Smith

Pachepsky

2023

Applied Microbiology

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Irrigation waters may facilitate the spread of antibiotic-resistant bacteria or genes to humans and animals. Monitoring of resistance in irrigated waters has become common; however, many studies do not incorporate a spatial component into sampling designs. The objective of this work was to assess spatiotemporal variations in tetracycline-resistant E. coli in an irrigation pond. Water samples were collected at 10 locations and two different water depths, and in situ and laboratory water quality measurements were performed. The percentage of E. coli resistant to the low (4 μg mL−1) and high (16 μg mL−1) tetracycline doses varied by date and location but were observed to be as high as 12.7% and 6.3% of the total population throughout the study, respectively. While significant differences were not observed between resistance levels measured at different depths, on one date resistant E. coli were only detected in samples collected at depth. Nitrate, fluorescent dissolved organic matter, and dissolved oxygen concentrations were found to be the leading control variables for the percentage of resistant E. coli. This work demonstrates that there may be substantial spatial variability in concentrations of antibiotic-resistant E. coli in irrigation ponds which should be accounted for in the design of monitoring programs.

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“…Statistically, more than 50% of bacterial infections around the world are due to MDR bacteria 79 . A long time ago, these MRD bacteria could transform, directly or indirectly, and cause a series of illnesses in humans and animals, such as S. aureus, E. coli, S. enteric, Bacillus cereus, and P. aeruginosa [80][81][82][83] . For instance, these reports demonstrated that more than 20% of clinically isolated E. coli are resistant to the first and second lines of treatments 79,84 .…”

Section: Discussionmentioning

confidence: 99%

Characterization, antibacterial, and cytotoxic activities of silver nanoparticles using the whole biofilm layer as a macromolecule in biosynthesis

Yakoup,

Kamel,

Elbermawy

et al. 2024

Sci Rep

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Recently, multi-drug resistant (MDR) bacteria are responsible for a large number of infectious diseases that can be life-threatening. Globally, new approaches are targeted to solve this essential issue. This study aims to discover novel antibiotic alternatives by using the whole components of the biofilm layer as a macromolecule to synthesize silver nanoparticles (AgNPs) as a promising agent against MDR. In particular, the biosynthesized biofilm-AgNPs were characterized using UV-Vis spectroscopy, electron microscopes, Energy Dispersive X-ray (EDX), zeta sizer and potential while their effect on bacterial strains and normal cell lines was identified. Accordingly, biofilm-AgNPs have a lavender-colored solution, spherical shape, with a size range of 20–60 nm. Notably, they have inhibitory effects when used on various bacterial strains with concentrations ranging between 12.5 and 25 µg/mL. In addition, they have an effective synergistic effect when combined with phage ZCSE9 to inhibit and kill Salmonella enterica with a concentration of 3.1 µg/mL. In conclusion, this work presents a novel biosynthesis preparation of AgNPs using biofilm for antibacterial purposes to reduce the possible toxicity by reducing the MICs using phage ZCSE9.

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Persistent Patterns of E. coli Concentrations in Two Irrigation Ponds from 3 Years of Monitoring

Cited by 11 publications

References 41 publications

Intra‐daily variation of Escherichia coli concentrations in agricultural irrigation ponds

Intra‐daily variation of Escherichia coli concentrations in agricultural irrigation ponds

Spatial Variation of Tetracycline-Resistant E. coli and Relationships with Water Quality Variables in Irrigation Water: A Pilot Study

Characterization, antibacterial, and cytotoxic activities of silver nanoparticles using the whole biofilm layer as a macromolecule in biosynthesis

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