Accident prevention is of great significance in avoiding or reducing all kinds of casualties and economic losses, and is one of the main challenges for social sustainable development. Hence, it has been an active research field for many decades around the world. To master the research status of accident prevention, and explore the knowledge base and hot trends, 1294 papers from the WOS retrieval platform SCIE and SSCI databases from 1990 to 2021 were selected as data samples. Co-occurrence analysis, co-citation analysis, co-authorship analysis, and keyword analysis were performed on the literature on accident prevention research with bibliometric analysis methods. The study showed that the United States ranked first in the number of publications of any country/region and Georgia Inst Technol ranked first in the number of institutional publications. System analysis and accident model establishment, analysis of construction accidents, road accident prevention, and safety culture and safety climate are the knowledge base in the accident prevention studies and the core journals in this field are Safety Science, Accident Analysis and Prevention, Pediatrics, and Reliability Engineering & System Safety. There are four major research hotspots in accident prevention studies: routine accident prevention, model-based research, systems analysis and accident prediction, and occupational safety and public health research. At present, the basic theory and structural system of accident prevention research have been basically established, with many research directions and a wide range of frontier branches. Safety management, public safety, Bayesian networks, and simulation are the research frontiers of accident prevention.