Personalizing the safe, appropriate and effective concentration(s) of ozone for a non-diabetic individual and four type II diabetic patients in autohemotherapy through blood hemoglobin analysis

Mehraban, Fouad; Seyedarabi, Arefeh; Ahmadian, Shahin; Mirzaaghaei, Vahid; Moosavi-Movahedi, Ali Akbar

doi:10.1186/s12967-019-1973-5

Cited by 13 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tumor immune microenvironment (TIME) is an important variable relating to the progression of CC. 11,12 Additionally, several immune prognostic signatures have been reported to predict the prognosis of patients with cancer, such as lung cancer, 13 ovarian cancer, 14 colorectal cancer, 15 and hepatocellular carcinoma. 16 These studies indicate that IRGs or TIME can serve as promising biomarkers for estimating survival in corresponding cancer.…”

Section: Introductionmentioning

confidence: 99%

Identification of a prognostic immune signature for cervical cancer to predict survival and response to immune checkpoint inhibitors

et al. 2019

View full text Add to dashboard Cite

2019) Identification of a prognostic immune signature for cervical cancer to predict survival and response to immune checkpoint inhibitors, OncoImmunology, 8:12, e1659094, ABSTRACT Cervical cancer (CC) is a leading cause of cancer-related death in women. Limited studies have investigated whether immune-related genes (IRGs) or tumor immune microenvironment (TIME) could be indicators for CC prognoses. The aim of this study was to develop an improved prognostic signature for CC based on IRGs or TIME to predict survival and response to immune checkpoint inhibitors (ICIs). A prognostic signature was constructed using bioinformatics method and its predictive capability was validated. The mechanisms underlying the signature's predictive capability were explored with CIBERSORT algorithm and mutation analysis. Immunophenoscore (IPS) is validated for ICIs response, and was therefore explored in relation to the signature. A prognostic signature based on 11 IRGs was developed. A multivariate analysis revealed that the 11-IRG signature was an independent prognostic factor for overall survival (OS) and progression-free interval in CC patients. In the 11-IRG signature highrisk group, CD8 T cells and resting mast cells, which are found to associate with better OS in our study, were lower; activated mast cells, associated with poorer OS, were higher, compared with the low-risk group. An IPS analysis suggested that the 11-IRG signature low-risk group, which possessed a higher IPS, represented a more immunogenic phenotype that was more inclined to respond to ICIs. In short, an 11-IRG prognostic signature for predicting CC patients' survival and response to ICIs was firmly established. The predictive capability of this model in CC requires further testing with the goal of better prognostic stratification and treatment management. ARTICLE HISTORY

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of a prognostic immune signature for cervical cancer to predict survival and response to immune checkpoint inhibitors

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Bioinformatics is a interdiscipline that combines computer science and life science, and it calculates and analyzes the underlying meaning in massive biological data by comprehensively using the theories of computer science, statistics, and biological science. [10][11][12] Many research institutions have provided open platforms and analysis software, and their data contain genomic information and functional integration that greatly facilitates researchers to integrate and analyze biological data and effectively reduces experimental expenses and time costs.…”

Section: Discussionmentioning

confidence: 99%

<p>Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer</p>

Xie¹,

Xiong²,

Li³

et al. 2019

OTT

View full text Add to dashboard Cite

Background: As one of the most common cancers, breast carcinoma is the most common disease in women. Intersectin 1 (ITSN1) contributes to the actin cytoskeleton reconstruction in breast cancer. Purpose: The objective of this study to explore the functions of ITSN1 in breast carcinoma. Methods: We downloaded microarray datasets GSE8087, GSE50697, and GSE98238 from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were used to construct a protein-protein interaction (PPI) network using STRING database, and the modules from PPI network were verified by Cytoscape software. Gene ontology terms and Kyoto Encyclopedia of Gene and Genome pathway were used to analyze the biological functions using the DAVID database. ONCOMINE, GEPIA, UALCAN, and Human Protein Atlas databases were used to investigate the characteristics of ITSN1 for the prognosis of breast carcinoma. Cell counting kit-8, flow cytometry, and colony formation assays were used to detect cell viability, cell apoptosis, and cell proliferation. RT-PCR and Western blot assays were used to detect ITSN1, Ki67, and cleaved caspase-3 expressions. Results: Low expressions of ITSN1 were significantly associated with clinical cancer stages. RT-PCR and Western blot analysis showed low expression of ITSN1 in breast cancer tissues and cell lines. ITSN1 inhibition could promote cell proliferation and inhibit cell apoptosis, while ITSN1 overexpression could inhibit cell proliferation and increase cell apoptosis by regulating the levels of expression of Ki67 and cleaved-caspase-3. Conclusion: The results indicated that ITSN1 could be a prognostic biomarker for survivals of breast cancer patients.

show abstract

“…To measure the DLS of Hb samples, the same method was used as reported in our previous study (Mehraban et al, 2019). The refractive index of the material (protein) and the absorption were set to 1.59 and 0.01, respectively.…”

Section: Dynamic Light Scatteringmentioning

confidence: 99%

“…Ozone dissolves rapidly in the aquatic environment of the blood and gives its energy to the most hematic portion of the blood, hemoglobin (Hb) (Bocci et al, 2011b). Recently, the effects of ozone on Hb have been extensively studied (Karnaukhova et al, 2014;Mehraban et al, 2018Mehraban et al, , 2019.…”

Section: Introductionmentioning

confidence: 99%

“…Structural changes in protein in the presence of multiple compounds can be explained by their respective interactions (Kamshad et al, 2019;Mokaberi et al, 2019Mokaberi et al, , 2020. In Hb, structural changes have been reported upon ozonation in different environments and under different conditions using multiple spectroscopic techniques (Mehraban et al, 2018(Mehraban et al, , 2019.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highlighting the Importance of Water Alkalinity Using Phosphate Buffer Diluted With Deionized, Double Distilled and Tap Water, in Lowering Oxidation Effects on Human Hemoglobin Ozonated at High Ozone Concentrations in vitro

Mehraban

Rayati

Mirzaaghaei³

et al. 2020

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

In autohemotherapy, it is important to find a way to lower the effects of oxidation, especially at high concentrations of ozone. One of the parameters, other than ozone concentration, which can have a significant effect on the stability and rate of decomposition of ozone at high concentrations, is the presence of ions in water. A number of spectroscopic techniques including intrinsic fluorescence, circular dichroism and UV-VIS were used as well as SDS-PAGE, Native-PAGE dynamic light scattering and water ion analysis, in order to investigate the effects of two relatively high concentrations of ozone on purified human hemoglobin (Hb) in phosphate buffer and diluted versions with deionized, double distilled and tap water in vitro. Purified human Hb and not whole blood human Hb was used in this study, since the addition of water to the whole blood would have caused the RBCs to lyse, affecting the purification of Hb for further analysis. Therefore, using purified Hb, it was possible to investigate the effects of dilution of 50 mM phosphate buffer to 10 mM phosphate buffer with different water types including non-ion containing deionized and double distilled water as well as ion-containing tap water, when ozonated at 55 and 80 µg/ml ozone. The fundamental changes in the secondary and tertiary structures of Hb were seen to be related to non-ozonated Hb samples diluted with deionized and double distilled waters, respectively. Generally, Hb oligomerization was more likely to occur at the higher concentration of ozone (80 µg/ml) and in samples where phosphate buffer was diluted with non-ion containing deionized and double distilled waters and not the ion-containing tap water. This could be explained by the presence of water alkalinity or bicarbonate ions in tap water, which can scavenge free radicals and reduce Hb oxidation/oligomerization. Therefore, it was concluded that

show abstract

Personalizing the safe, appropriate and effective concentration(s) of ozone for a non-diabetic individual and four type II diabetic patients in autohemotherapy through blood hemoglobin analysis

Cited by 13 publications

References 38 publications

Identification of a prognostic immune signature for cervical cancer to predict survival and response to immune checkpoint inhibitors

Identification of a prognostic immune signature for cervical cancer to predict survival and response to immune checkpoint inhibitors

<p>Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer</p>

Highlighting the Importance of Water Alkalinity Using Phosphate Buffer Diluted With Deionized, Double Distilled and Tap Water, in Lowering Oxidation Effects on Human Hemoglobin Ozonated at High Ozone Concentrations in vitro

Contact Info

Product

Resources

About