Perspective of Micropropagation Industry

Chu, Irwin Y. E.

doi:10.1007/978-94-011-2785-1_8

Cited by 22 publications

(11 citation statements)

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“…In general, for the conservation of valuable medicinal plant resources, micropropagation technology through axillary bud proliferation has proven to be a handy tool (Pierik, 1978;Mantell et al, 1985;Chu, 1992;Malathy and Pai, 1998). Furthermore, in vitro techniques offer the possibility of rapid clonal propagation of important plants allowing production of genetically stable and trueto-type progeny (Hu and Wang, 1983).…”

Section: Introductionmentioning

confidence: 97%

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Selvakumar

Anbudurai

Balakumar

2001

In Vitro Cell.Dev.Biol.-Plant

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A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced on Murashige and Skoog's basal medium supplemented with 27.2 mM adenine sulfate +2.46 mM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing 4.92 mM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period of 4 wk, there was a 90% transplantation success in the field compared to the 60±65% survival of plantlets recorded in the experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants.

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Section: Introductionmentioning

confidence: 97%

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Selvakumar

Anbudurai

Balakumar

2001

In Vitro Cell.Dev.Biol.-Plant

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“…The simplest category of in vitro plant propagation is the stimulation of axillary bud development/proliferation which shows the least variation among the micropropagated plants [16]. The technique exploits the usual ontogenic route of branch development by lateral (axillary meristems).…”

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination with α-napthalene acetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium were tested. The highest regeneration frequency (90 %) with optimum number of shoots (32 ± 0.00)/explant were obtained on MS medium fortified with 2.5 μM 6-benzyladenine (BA) and 0.5 μM NAA and 30 g/l sucrose at pH 5.8. Among the tried TDZ concentrations, 0.5 μM resulted in maximum number of shoots (20.4 ± 0.40)/explant after 4 weeks of exposure. The proliferating shoot cultures established by repeated subculturing of the mother explants on the hormone-free medium produced the highest shoot number (29.4 ± 0.40) with shoot length (6.80 ± 0.12 cm)/explant at fourth subculture passage, which a decline in shoot proliferation was recorded. Different concentrations of NAA were tested for ex vitro rooting of microshoots. The maximum percentage of rooting 100 % with maximum roots (18.3 ± 0.1) was achieved in soilrite when basal portion of the microshoots were treated with 200 μM (NAA) for 15 min per shoot. The plantlets went through hardening phase in a growth chamber, prior to ex vitro transfer. The PCR-based single primer amplification reaction (SPAR) methods which include random amplified polymorphic DNA (RAPD) and direct amplification of minisatellite DNA (DAMD) markers has been used for assessment of genetic stability of micropropagated plantlets. No variation was observed in DNA fingerprinting patterns among the micropropagated and the donor plants illustrating their genetic uniformity.

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“…Node culture has been previously adopted to propagate in vitro shrubs and trees (George, 1996;Suttle, 1999). This method is considered convenient and reliable because using pre-existing bud explants it is least likely to induce genetic mutation (Chu, 1992), and therefore it is a preferred system for commercial micropropagation (Suttle, 1999). The highest rate of axillary shoot proliferation was induced on MS medium augmented with 0.44 µ M BA.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of <i>Genista aetnensis</i> [(Raf. ex Biv.)DC]

Iapichino

AIRÒ²,

Presti

et al. 2015

Not Bot Hort Agrobot Cluj

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Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi-hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis. The Murashige and Skoog medium, augmented with different concentrations of N -6 -benzyladenine either singly or in combination with indole-3-acetic acid, as potential medium for shoot multiplication by nodal segments was tested. In the following experiment equal molar concentrations of four cytokinins (2-isopenthenyladenine, kinetin, zeatin and N -6 -benzyladenine) were tested for ability to induce axillary shoot development from single node stem segments. The highest rate of axillary shoot proliferation was induced on the medium supplemented with 0.44 μM BA. Growth regulator requirements for shoot proliferation in G. aetnensis were satisfied by BA alone. Explants were divided, subcultured and continued to proliferate shoots. A proliferation rate of 3.5 shoots per single node explants every four weeks occurred. Seven indole-3-acetic acid concentrations (0, 0.23, 0.45, 0.91, 1.82, 3.64 or 7.29 µM) were tested to determine the optimum conditions for in vitro rooting of microshoots. The highest rooting percentage was obtained with indole-3-acetic acid at 3.64 µM (57%). Eighty percent of the in vitro rooted plantlets were successfully established in soil. This micropropagation system of G. aetnensis based on axillary shoot development from nodal segments followed by in vitro rooting should be preferred for rapid and efficient mass propagation of selected clones and could represent an alternative method to sexual and conventional asexual propagation.

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Perspective of Micropropagation Industry

Cited by 22 publications

References 0 publications

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Micropropagation of <i>Genista aetnensis</i> [(Raf. ex Biv.)DC]

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