Perspectives of genetically engineered microbes for groundwater bioremediation

Janssen, Dick B.; Stucki, Gerhard

doi:10.1039/c9em00601j

Cited by 66 publications

(18 citation statements)

References 70 publications

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“…High salinity causes plasmolysis and cell lysis due to increase of osmotic potential and affects the metabolic activities of the degrading microbes [ 60 , 61 ]. Nevertheless, salinity tolerance of YC-IL1 strain can be improved with mutagenesis and genetic engineering tools [ 62 ]. Altogether, these properties indicate that YC-IL1 is a novel strain capable of bioremediating PAEs pollutants from environments under different conditions.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

Lamraoui

Eltoukhy

Wang

et al. 2020

IJERPH

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Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems. Enterobacter spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.

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Section: Discussionmentioning

confidence: 99%

Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

Lamraoui

Eltoukhy

Wang

et al. 2020

IJERPH

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“…While engineering organisms for environmental deployment is a new frontier, we note that it is not without precedent, as there are many such research efforts underway (Coleman and Goold, 2019;Jaiswal and Shukla, 2020;Janssen and Stucki, 2020;Rylott and Bruce, 2020;Zhou et al, 2022), including field trials (Carvalho et al, 2015). Advances in biocontainment may provide options for restricting the spread of engineered microbes outside the intended environment (Lee et al, 2018).…”

Section: Toward Further Integration Of Biology Into Geochemical Netsmentioning

confidence: 99%

Geochemical Negative Emissions Technologies: Part I. Review

et al. 2022

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Over the previous two decades, a diverse array of geochemical negative emissions technologies (NETs) have been proposed, which use alkaline minerals for removing and permanently storing atmospheric carbon dioxide (CO2). Geochemical NETs include CO2 mineralization (methods which react alkaline minerals with CO2, producing solid carbonate minerals), enhanced weathering (dispersing alkaline minerals in the environment for CO2 drawdown) and ocean alkalinity enhancement (manipulation of ocean chemistry to remove CO2 from air as dissolved inorganic carbon). CO2 mineralization approaches include in situ (CO2 reacts with alkaline minerals in the Earth's subsurface), surficial (high surface area alkaline minerals found at the Earth's surface are reacted with air or CO2-bearing fluids), and ex situ (high surface area alkaline minerals are transported to sites of concentrated CO2 production). Geochemical NETS may also include an approach to direct air capture (DAC) that harnesses surficial mineralization reactions to remove CO2 from air, and produce concentrated CO2. Overall, these technologies are at an early stage of development with just a few subjected to field trials. In Part I of this work we have reviewed the current state of geochemical NETs, highlighting key features (mineral resources; processes; kinetics; storage durability; synergies with other NETs such as DAC, risks; limitations; co-benefits, environmental impacts and life-cycle assessment). The role of organisms and biological mechanisms in enhancing geochemical NETs is also explored. In Part II, a roadmap is presented to help catalyze the research, development, and deployment of geochemical NETs at the gigaton scale over the coming decades.

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“…Remediation of contained liquids allows for a high degree of process control and engineered bacteria can be employed using established microbial fermentation technologies. These advantages can also be extended to field applications when dealing with contaminated liquids that can be diverted into a contained system, such as extractable groundwater (Janssen and Stucki, 2020). New genetic tools for engineering different bacteria are reported regularly, and as such there are increasing options to engineer reductive P450 metabolism into bacteria that can already perform downstream steps in a bioremediation metabolic pathway.…”

Section: New Frontiers and Future Directions In Synthetic Biology For Bioremediationmentioning

confidence: 99%

Reductive Cytochrome P450 Reactions and Their Potential Role in Bioremediation

Behrendorff

2021

Front. Microbiol.

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Cytochrome P450 enzymes, or P450s, are haem monooxygenases renowned for their ability to insert one atom from molecular oxygen into an exceptionally broad range of substrates while reducing the other atom to water. However, some substrates including many organohalide and nitro compounds present little or no opportunity for oxidation. Under hypoxic conditions P450s can perform reductive reactions, contributing electrons to drive reductive elimination reactions. P450s can catalyse dehalogenation and denitration of a range of environmentally persistent pollutants including halogenated hydrocarbons and nitroamine explosives. P450-mediated reductive dehalogenations were first discovered in the context of human pharmacology but have since been observed in a variety of organisms. Additionally, P450-mediated reductive denitration of synthetic explosives has been discovered in bacteria that inhabit contaminated soils. This review will examine the distribution of P450-mediated reductive dehalogenations and denitrations in nature and discuss synthetic biology approaches to developing P450-based reagents for bioremediation.

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Perspectives of genetically engineered microbes for groundwater bioremediation

Abstract: Bacteria degrading 1,2,3-trichloropropane were constructed by genetic engineering and may be applicable for groundwater bioremediation, following the example of 1,2-dichloroethane.

Cited by 66 publications

References 70 publications

Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

Geochemical Negative Emissions Technologies: Part I. Review

Reductive Cytochrome P450 Reactions and Their Potential Role in Bioremediation

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