Perspectives of nanotechnology in male fertility and sperm function

Falchi, Laura; Khalil, Wael A.; Hassan, Mahmoud A. E.; Marei, Waleed F.A.

doi:10.1016/j.ijvsm.2018.09.001

Cited by 83 publications

(56 citation statements)

References 81 publications

(93 reference statements)

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“…Nanotechnology, such as nano-zinc and nano-selenium, can be used to achieve bioactive properties in the reproduction, digestion, development, and freezing cells of various elements [78]. Recent studies have shown the beneficial effects of nanoparticles for sperm freezing in humans and animals by lowering the sperm chromatin damage and MDA levels [79][80][81]. However, no studies have examined the impact of zinc oxide or selenium nanoparticles on epididymal camel sperm.…”

Section: Discussionmentioning

confidence: 99%

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supplementation with vitamin C, E, inorganic trace elements of selenium (Na2SeO3) and zinc (ZnSO4), and zinc and selenium nanoparticles (ZnONPs and SeNPs, respectively) on the cryopreservation of dromedary camel epididymal spermatozoa. When the SHOTOR extender was supplemented with ZnONPs and SeNPs; the sperm showed increased progressive motility; vitality; and membrane integrity after cooling at 5 °C for 2 h; when compared to the control and vitamin-supplemented groups. Moreover, the ZnONPs and SeNPs supplementation improved the progressive motility, vitality, sperm membrane integrity, ultrastructural morphology, and decreased apoptosis when frozen and thawed. SeNPs significantly increased reduced glutathione (GSH), superoxide dismutase (SOD), and decreased lipid peroxide malondialdehyde (MDA) levels. The advantageous effects of the trace elements were potentiated by reduction into a nano-sized particle, which could increase bioavailability and reduce the undesired liberation of toxic concentrations. We recommend the inclusion of SeNPs or ZnONPs to SHOTOR extenders to improve the cryotolerance of camel epididymal spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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“…In this study, the synthesized iron oxide particles measured 7 to 14 nm in diameters, and corresponded to maghemite (γ-Fe 2 O 3 ) and hematite (α-Fe 2 O 3 ) as revealed by the HRTEM images showing respective interplanar distances of 2.54 Å and 2.98 Å [41, 42]. All forms of MNP (hematite or α-Fe 2 O 3 , maghemite or γ-Fe 2 O 3 , and magnetite or Fe 3 O 4 ) have been used for in vitro targeting of spermatozoa, in a broad range of applications (i.e., sperm nanopurification, toxicity test, and sperm-mediated gene transfer), without affecting their functionality or structures (reviewed by [43]). In the current study, the conjugation of maghemite-made MNP with annexin V and hematite-made MNP with lectins (PNA/LCA) to respectively target apoptici and acrosome reacted spermatozoa [44–46] did not affect the MNP magnetism nor the functionality of both MNP conjugates.…”

Section: Discussionmentioning

confidence: 99%

Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Durfey

Swistek

Liao

et al. 2019

J Animal Sci Biotechnol

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BackgroundAdvances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles (MNP). Their use to detect and remove damaged spermatozoa from semen doses could be of great interest. Here, MNP were synthesized and tested for their ability to target apoptotic (annexin V) and acrosome-reacted (lectin) boar spermatozoa, for high-throughout retrieval in a magnetic field (nanoselection). The potential impacts of nanoselection on sperm functions and performance of offspring sired by sperm subjected to nanoselection were determined. Fresh harvested and extended boar semen was mixed with various amounts (0, 87.5, and 175 μg) of MNP-conjugates (Annexin V-MNP or Lectin-MNP) and incubated (10 to 15 min) for 37 °C in Exp. 1. In Exp. 2, extended semen was mixed with optimal concentrations of MNP-conjugates and incubated (0, 30, 90, or 120 min). In Exp. 3, the synergistic effects of both MNP-conjugates (87.5 μg – 30 min) on spermatozoa was evaluated, followed by sperm fertility assessments through pregnancy of inseminated gilts and performance of neonatal offspring. Sperm motion, viability, and morphology characteristics were evaluated in all experiments.ResultsTransmission electron microscopy, atomic force microscopy, and hyperspectral imaging techniques were used to confirm attachment of MNP-conjugates to damaged spermatozoa. The motility of nanoselected spermatozoa was improved (P < 0.05). The viability of boar sperm, as assessed by the abundance of reactive oxygen species and the integrity of the acrosome, plasma membrane, and mitochondrial membrane was not different between nanoselected and control spermatozoa. The fertility of gilts inseminated with control or nanoselected spermatozoa, as well as growth and health of their offspring were not different between (P > 0.05).ConclusionsThe findings revealed the benefit of magnetic nanoselection for high-throughput targeting of damaged sperm, for removal and rapid and effortless enrichment of semen doses with highly motile, viable, and fertile spermatozoa. Therefore, magnetic nanoselection for removal of abnormal spermatozoa from semen is a promising tool for improving fertility of males, particularly during periods, such as heat stress during the summer months.

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“…Darbandi et al () reported that lifestyle and environmental factors might be major contributing factors to the rising incidence of male infertility. The scientific community is also increasingly wary of the deleterious effects of nanomaterials on the reproductive system, especially of males (Falchi, Khalil, Hassan, & Marei, ). This current study, therefore, assessed the potential reproductive toxicity of VERN following 14‐days repeated dose administration in male albino rats.…”

Section: Discussionmentioning

confidence: 99%

Salubrious effects of a vermiculite–cellulose‐based bionanocomposite on oxidative stress indices and histomorphology of male Wistar rats

et al. 2019

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There is a current interest from the food packaging, biomedical and agricultural sectors in hybrid materials formed from clays and natural polymeric compounds. However, research investigating the toxicity of vermiculite–cellulose nanocrystal (VERN) hybrid on the testes of Wistar rats is rare. Twenty rats, divided into control and treatment groups, were orally administered distilled water, 5, 10, and 20 mg/kg bw VERN daily for two consecutive weeks. At the termination of experiments, the testicular organo‐somatic index, superoxide dismutase, catalase, and gamma‐glutamyl transferase activities were not significantly changed by VERN relative to the controls. Contrarily, myeloperoxidase, glutathione peroxidase, and malondialdehyde levels were depleted in the testes of treated rats. Moreso, VERN increased follicle‐stimulating and luteinizing hormones, and decreased testosterone levels at the 20 mg/kg dose. Histology of the testes revealed healthy looking Leydig cells at the doses of 5 and 10 mg/kg VERN. Overall, these results indicate that oral exposure of VERN was not overly deleterious to the redox and structural histoarchitecture in the testes of rats.

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Perspectives of nanotechnology in male fertility and sperm function

Cited by 83 publications

References 81 publications

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Salubrious effects of a vermiculite–cellulose‐based bionanocomposite on oxidative stress indices and histomorphology of male Wistar rats

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