Perspectives of Use Laser Doppler Flowmetry for Accurate Determination Оf Degree of a Burn at Prehospital Stage

Юрова, Ю. В.; Zinoviev, E. V.; Krylov, K. M.; Soloshenko, V. V.

doi:10.24884/2072-6716-2019-20-4-33-36

Cited by 2 publications

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“…Ранее Ю. В. Юрова и др. отметили, что показатель микроциркуляции в гранулирующей ране больше, чем в здоровой коже [18,36]. Это, вероятно, обусловлено барьерной функцией эпидермиса и отсутствием в нем капилляров.…”

Section: мышиunclassified

“…Запись продолжали в течение 3 мин. Оценку параметров микроциркуляции донорской раны проводили по одному из основных показателей микроциркуляции -М (среднее арифметическое значение показателя микроциркуляции, измеряемое в перфузионных единицах (ПЕ))[18].Гистологическое исследованиеНа 14 сутки эксперимента выполняли биопсию в пределах эпителизированных донорских участков на всю глубину до фасции и мышц с помощью DERMO-PUNCH DMP-35 (STERYLAB, Италия). Биоптаты регенерирующих тканей подвергали стандартной гистологической обработке с последующей окраской гематоксилин-эозином и анилиновым синим по Массону.…”

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Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Koloshein,

Ryabkov,

Egorikhina

et al. 2024

Урал. мед. журнал

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Introduction. The optimal method of obtaining and application a stromal-vascular fraction (SVF) of adipose tissue in the treatment of donor wounds in burned patients has not been determined.Objective — to compare the effectiveness of the stromal-vascular fraction of adipose tissue in the treatment of donor wounds, depending on the method of obtaining (mechanical and enzymatic) and the injection (subdermal, intradermal) into the tissue.Material and methods. Wistar rats (n = 30) were divided into 2 investigated groups: in the first group (eSVF) enzymatic processing of adipose tissue (n = 10) was used, in the second group (mSVF) mechanical processing was used (n = 10). Another 10 rats were used as adipose tissue donors’ group. A deep burn was created in all animals, and after 4 days two more donor wounds were created by taking a split thickness skin autograft: the stromal-vascular fraction was injected intradermally into one of the wounds, subdermally into the other. After 14 days, the area of epithelialization, the microcirculation index and the histological microstructure of the skin were evaluated.Results. The proportion of completely epithelized donor wounds in the eSVF-group was 85 %, in the mSVF-group — 55 % (p < 0.05). The index of microcirculation significantly decreased after intradermal injections in the eSVF-group (p < 0.01), and after subdermal injections — in the mSVF-group (p < 0.05). According to histomorphometry, with intradermal injections of eSVF, the thickness of the epidermis and the layer of granulation tissue is less than in the mSVF group (p < 0.01). At the same time, the relative density of collagen fibers in the granulation tissue was significantly higher after the injections of eSVF than after mSVF (p < 0.01). Comparison of injections of the stromal-vascular fraction showed: no significant differences were found when using eSVF; when using mSVF, the subdermal injections are preferable, since it was accompanied by a significantly lower thickness of granulation tissue (p < 0.01).Conclusions. The enzymatically obtained stromal-vascular fraction of adipose tissue has advantages over the mechanically obtained one: the efficiency of eSVF is equally high when used with intradermally and subdermally injections, which are confirmed by the structure and blood circulation in the newly formed skin. When using mSVF, intradermal injections are the least effective, and subdermal injections are accompanied by a moderately pronounced positive dynamic in the microstructure of the skin of donor wounds.

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Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Koloshein,

Ryabkov,

Egorikhina

et al. 2024

Урал. мед. журнал

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Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

Durnovo,

Tarakanova,

Shakhova

et al. 2023

Kuban. nauсh. med. vestnik

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Background. Healing of open wounds in the oral cavity is often accompanied by infection and constant traumatization, which lead to impaired blood supply to the tissues, lack of trophic support and low oxygen supply to increasing proliferative processes. Recently, photodynamic therapy has taken its place among the methods for stimulating regeneration of oral mucosal tissues. The authors consider it essential to study the influence of photodynamic treatment on the functional state of the microvasculature in the wound tissues, which determines the transcapillary exchange and the tissue respiration, providing the tissue regeneration.Objective. To study the photodynamic effect of sequential dual-wavelength radiation on the microvasculature in the wound tissues of the oral mucosa in vivo.Methods. The study was carried out on 36 female outbred Wistar rats, weighing 200–250 g. The animals were divided into two groups: control group (n = 18) and experimental group (n = 18). Wound surfaces healed by secondary intention were modeled on the cheek mucosa of the animals. Dual-wavelength photodynamic therapy was performed in the 1st postoperative day in experimental group. The microcirculatory function was assessed by Doppler flowmetry before, and on days 3, 7 and 14 after wounding. The results were processed using IBM SPSS Statistics 26.0.0.1 (IBM Inc., USA).Results. It has been established that dual-wavelength photodynamic therapy has a positive effect on the restoration of the microvasculature of the wound tissues. This therapy facilitated bypassing for microcirculatory flow on the 3rd day of the study, and increased the perfusion of tissues on the 7th. The experimental group did not have congestive-ischaemic phenomena in the wound region; the microcirculatory flow was augmented through the microcirculation regulation by the 14th day.Conclusion. Following the obtained results the use of dual-wavelength photodynamic therapy can be considered as a promising method for restoration of the microvasculature of tissues in the oral mucosal trauma.

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Perspectives of Use Laser Doppler Flowmetry for Accurate Determination Оf Degree of a Burn at Prehospital Stage

Cited by 2 publications

References 2 publications

Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

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