Perspectives on Hormonal Manipulation of Shrimp Reproduction

Okumura, Takuji

doi:10.6090/jarq.38.49

Cited by 62 publications

(27 citation statements)

References 24 publications

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“…Testes contained immature sperm cells indicated by undeveloped spike at the anterior of the spermatozoa. The pattern of spermatozoa development observed in the present tudy was relatively similar to that found on giant freshwater shrimp, Macrobrachium rosenbergii (Okumura, 2004), lobster, Nephrops norvergicus (Rotllant et al, 2012) and white shrimp, Litopenaeus schmitti (Fransozo et al, 2016). Based on morphological observation on spermatozoa cell, it was also identified undeveloped spikespermatozoa inside spermatophore.…”

Section: Discussionsupporting

confidence: 51%

Reproductive performances of wild male tiger shrimp, Penaeus monodon post-injection of oocyte developer without eyestalk ablation

Prasetyo

Laining²,

Sudrajat

2017

Jurnal Akuakultur Indonesia

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The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5) and 1 (OD1.0) mL/kg of body weight and a control was eyestalk ablation (AB). The male stock of tiger shrimp used was from wild with body weight ranged from 55-85 g, stocked into three of 10 tonnages concrete tanks with density of 25 males/tank. Oodev injection was applied for two times with one week interval. Tiger shrimp of OD0.5 group produced the highest number of gonadal maturing which was 84% followed by AB (68%) and OD1.0 (64%). Oodev injection was able to shorten the time required for spermatophore maturation at maturation phase which happened simultaneously within 7 days post injection compared to that of ablated males. Positive correlation (R2=0.612) was detected between the shrimp weight and weight of spermatophore of the tiger shrimp while correlation between spermatophore weight and number of spermatozoa was relatively low (R2=0.415). Total fatty acid tented to be higher in males injected with oodev compared to ablated males. Concentration of arachidonic acid in the muscle of male stock in OD1.0 group was extremely low of 0.0037% of total lipid compared to AB (0.3190%) and OD0.5 (0.2806%). Oodev injection at the dose of 0.5 mL/kg of tiger shrimp could improve the number of males stock producing spermatophore compared to eyestalk ablation. Simultaneously matured-spermatophore of wild male tiger shrimp within short time could be achieved through oodev injection.Keywords: oodev, spermatophore, reproduction, tiger shrimp ABSTRAK Tujuan penelitian ini adalah mengevaluasi efek hormon oodev terhadap pematangan gonad dan karakter spermatofor dan spermatozoa yang dihasilkan oleh induk udang windu jantan alam dibandingkan dengan teknik ablasi tangkai mata. Perlakuan yang dicobakan adalah dua dosis injeksi oodev yaitu 0,5 (OD0,5) dan 1 (OD1,0) mL/kg bobot tubuh dan kontrol yaitu ablasi tangkai mata (AB). Induk udang windu jantan yang digunakan berasal dari alam berbobot antara 55-85 g, ditebar dalam tiga bak pematangan gonad berkapasitas 10 ton dengan kepadatan 25 ekor/ bak. Injeksi oodev dilakukan dua kali dengan interval satu minggu. Induk udang windu pada OD0,5 menghasilkan jumlah induk matang gonad tertinggi yaitu 84%, diikuti oleh AB (68%) dan OD1 (64%). Injeksi oodev mampu mempersingkat masa pematangan spermatofor pada fase maturasi dan pematangan tersebut terjadi secara simultan setelah 7 hari pascainjeksi dibandingkan dengan induk yang di ablasi. Korelasi positif (R2=0,612) dideteksi antara bobot tubuh dan bobot spermatofor induk udang windu, sedangkan korelasi antara bobot spermatofor dan jumlah spermatozoa relatif lemah (R2=0,415). Total asam lemak daging cenderung lebih besar pada induk udang windu jantan yang diinjeksi oodev dibandingkan yang diablasi. Konsentrasi arachidonic acid dalam daging induk yang diinjeksi OD1 sangat...

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Section: Discussionsupporting

confidence: 51%

Reproductive performances of wild male tiger shrimp, Penaeus monodon post-injection of oocyte developer without eyestalk ablation

Prasetyo

Laining²,

Sudrajat

2017

Jurnal Akuakultur Indonesia

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“…Acting opposite to the inhibitory action of GIH is a putative ovarian stimulating factor produced by the brain and thoracic ganglia, that possibly stimulates vitellogenesis. This factor, though not yet characterized, has been called gonad-stimulating hormone (GSH) (Okumura, 2004;Meeratana et al, 2006). However, vitellogenin-stimulating ovarian hormone (VSOH) (Takayanagi et al, 1986;Yano et al, 1988) also plays a part in ovarian maturation.…”

Section: Introductionmentioning

confidence: 99%

Gonadotropin-releasing hormone (GnRH) and a GnRH analog induce ovarian maturation in the giant freshwater prawn,Macrobrachium rosenbergii

Ngernsoungnern

Sobhon³

et al. 2009

Invertebrate Reproduction & Development

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Gonadotropin-releasing hormone (GnRH) is a highly conserved peptide that plays a role in regulating reproduction in both vertebrates and invertebrates. The present study investigated the effects of lamprey (l)GnRH-I, lGnRH-III, octopus (oct)GnRH and buserelin, a GnRH analog (GnRHa), on ovarian maturation and spawning in the giant freshwater prawn, Macrobrachium rosenbergii. We demonstrated that the times for ovarian maturation in prawns treated with octGnRH (at 50 and 500 ng/g BW), lGnRH-I and lGnRH-III (at 500 ng/g BW), and GnRHa (at 1,000 ng/g BW) were significantly shorter (23.50 ± 2.12 and 22.50 ± 1.15, 25.50 ± 4.04 and 25.50± 4.03, and 26.67 ± 4.04 days) than the controls (40.0 ± 3.40 days). On day 22 post-treatment, the gonadosomatic index (GSI) values of the prawns treated with octGnRH (at 50 and 500 ng/g BW), lGnRH-I and lGnRH-III (at 500 ng/g BW), and GnRHa (at 1,000 ng/g BW) were significantly greater (2.50 ± 0.72 and 4.64 ± 0.95, 6.07 ± 1.29 and 8.90 ± 1.04, and 3.88 ± 1.34%) than the controls (0.45 ± 0.15%). Vitellin protein was first detected in the ovary on day 15 and had increased significantly by day 22 in prawns treated with GnRHs at 500 ng/g BW, while it was not detected in the controls. The prawns treated with the GnRHs and GnRHa showed similar numbers and percentages of spawned and fertilized eggs to those of the control group. These findings indicate that GnRH controls ovarian maturation and spawning in this prawn, as in other species.

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“…This in turn is integrated with olfaction stimuli via the connections between the protocerebral neuropils, namely the anterior medial protocerebral neuropils (AMPN), posterior medial protocerebral neuropils (PMPN), central body (CB), and protocerebral bridge (PB), with the olfactory neuropil (ON) and accessory neuropil (Ac) (Sullivan and Beltz 2001). The hormones controlling molting and reproduction in the CHH family are also produced in the X-organ-sinus gland (XO-SG) complex (Adiyodi 1985;Keller 1992;Huberman 2000;Okumura 2004;Mazurova et al 2008). Thus, the presence of ALP-ir only in XO-SG is likely to be related to the control of synthesis and release of the aforementioned neurohormones.…”

Section: Discussionmentioning

confidence: 99%

The distribution of APGWamide and RFamides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii

et al. 2011

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Immunohistochemistry was used to identify the distribution of both APGWamide-like and RFamide-like peptides in the central nervous system (CNS) and ovary of the mature female giant freshwater prawn, Macrobrachium rosenbergii. APGWamide-like immunoreactivity (ALP-ir) was found only within the sinus gland (SG) of the eyestalk, in small- and medium-sized neurons of cluster 4, as well as their varicosed axons. RFamide-like immunoreactivity (RF-ir) was detected in neurons of all neuronal clusters of the eyestalk and CNS, except clusters 1 and 5 of the eyestalk, and dorsal clusters of the subesophageal, thoracic, and abdominal ganglia. The RF-ir was also found in all neuropils of the CNS and SG, except the lamina ganglionaris. These immunohistochemical locations of the APGWamide-like and RF-like peptides in the eyestalk indicate that these neuropeptides could modulate the release of the neurohormones in the sinus gland. The presence of RFamide-like peptides in the thoracic and abdominal ganglia suggests that it may act as a neurotransmitter which controls muscular contractions. In the ovary, RF-ir was found predominantly in late previtellogenic and early vitellogenic oocytes, and to a lesser degree in late vitellogenic oocytes. These RFs may be involved with oocyte development, but may also act with other neurohormones and/or neurotransmitters within the oocyte in an autocrine or paracrine manner.

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Perspectives on Hormonal Manipulation of Shrimp Reproduction

Cited by 62 publications

References 24 publications

Reproductive performances of wild male tiger shrimp, Penaeus monodon post-injection of oocyte developer without eyestalk ablation

Reproductive performances of wild male tiger shrimp, Penaeus monodon post-injection of oocyte developer without eyestalk ablation

Gonadotropin-releasing hormone (GnRH) and a GnRH analog induce ovarian maturation in the giant freshwater prawn,Macrobrachium rosenbergii

The distribution of APGWamide and RFamides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii

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