Perspectives on the Feasibility of Using Enzymes for Pharmaceutical Removal in Wastewater

Bilal, Muhammad; Iqbal, Hafiz M.N.; Barceló, Damià

doi:10.1007/698_2020_661

Cited by 9 publications

(4 citation statements)

References 77 publications

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“…The next investigated factor, that may affect the efficiency of antibiotic degradation, was the concentration of H 2 O 2 , as hydrogen peroxide acts as a co-substrate for horseradish peroxidase ( Figure 3 e) [ 33 ]. Over the whole analyzed H 2 O 2 concentration range, free enzymes showed higher removal rates than immobilized HRP.…”

Section: Resultsmentioning

confidence: 99%

Removal of Persistent Sulfamethoxazole and Carbamazepine from Water by Horseradish Peroxidase Encapsulated into Poly(Vinyl Chloride) Electrospun Fibers

Zdarta

Degórska

Jankowska

et al. 2021

IJMS

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Enzymatic conversion of pharmaceutically active ingredients (API), using immobilized enzymes should be considered as a promising industrial tool due to improved reusability and stability of the biocatalysts at harsh process conditions. Therefore, in this study horseradish peroxidase was immobilized into sodium alginate capsules and then trapped into poly(vinyl chloride) electrospun fibers to provide additional enzyme stabilization and protection against the negative effect of harsh process conditions. Due to encapsulation immobilization, 100% of immobilization yield was achieved leading to loading of 25 μg of enzyme in 1 mg of the support. Immobilized in such a way, enzyme showed over 80% activity retention. Further, only slight changes in kinetic parameters of free (Km = 1.54 mM) and immobilized horseradish peroxidase (Km = 1.83 mM) were noticed, indicating retention of high catalytic properties and high substrate affinity by encapsulated biocatalyst. Encapsulated horseradish peroxidase was tested in biodegradation of two frequently occurring in wastewater API, sulfamethoxazole (antibiotic) and carbamazepine (anticonvulsant). Over 80% of both pharmaceutics was removed by immobilized enzyme after 24 h of the process from the solution at a concentration of 1 mg/L, under optimal conditions, which were found to be pH 7, temperature 25 °C and 2 mM of H2O2. However, even from 10 mg/L solutions, it was possible to remove over 40% of both pharmaceuticals. Finally, the reusability and storage stability study of immobilized horseradish peroxidase showed retention of over 60% of initial activity after 20 days of storage at 4 °C and after 10 repeated catalytic cycles, indicating great practical application potential. By contrast, the free enzyme showed less than 20% of its initial activity after 20 days of storage and exhibited no recycling potential.

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Section: Resultsmentioning

confidence: 99%

Removal of Persistent Sulfamethoxazole and Carbamazepine from Water by Horseradish Peroxidase Encapsulated into Poly(Vinyl Chloride) Electrospun Fibers

Zdarta

Degórska

Jankowska

et al. 2021

IJMS

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“…One group of ECs of particular concern is the estrogenic hormones, which include estrone, estriol, 17β-estradiol, and 17α-ethinylestradiol (Bilal et al, 2020a). The occurrence of these compounds in the environment is beginning to receive considerable attention from the scientific community and from public health and ecological conservation authorities.…”

Section: Highlightsmentioning

confidence: 99%

A contemporary review of enzymatic applications in the remediation of emerging estrogenic compounds

Zdarta

Nguyen

Jankowska

et al. 2021

Critical Reviews in Environmental Science and Technology

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“…Due to the increasing environmental pollution, which is majorly caused by anthropogenic activities (man-made processes), the people are now more concerned around the globe. The increasing number of various types of organic pollutants, including phenols, pharmaceuticals, pesticides, dyes, estrogens, and personal care products, is increasingly being detected in our water systems and wastewater [1][2][3]. Their presence in unknown and uncontrolled dosages undesirably compromises the quality of water and shows a serious risk to human beings and aquatic organisms [4,5].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Wastewater Treatment by Immobilized Enzymes

et al. 2021

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Purpose of Review In the presented review, we have summarized recent achievements on the use of immobilized oxidoreductases for biodegradation of hazardous organic pollutants including mainly dyes, pharmaceuticals, phenols, and bisphenols. In order to facilitate process optimization and achievement of high removal rates, effect of various process conditions on biodegradation has been highlighted and discussed. Recent Findings Current reports clearly show that immobilized oxidoreductases are capable of efficient conversion of organic pollutants, usually reaching over 90% of removal rate. Further, immobilized enzymes showed great recyclability potential, allowing their reuse in numerous of catalytic cycles. Summary Collected data clearly indicates immobilized oxidoreductases as an efficient biocatalytic tools for removal of hazardous phenolic compounds, making them a promising option for future water purification. Data shows, however, that both immobilization and biodegradation conditions affect conversion efficiency; therefore, process optimization is required to achieve high removal rates. Nevertheless, we have demonstrated future trends and highlighted several issues that have to be solved in the near-future research, to facilitate large-scale application of the immobilized oxidoreductases in wastewater treatment.

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Perspectives on the Feasibility of Using Enzymes for Pharmaceutical Removal in Wastewater

Cited by 9 publications

References 77 publications

Removal of Persistent Sulfamethoxazole and Carbamazepine from Water by Horseradish Peroxidase Encapsulated into Poly(Vinyl Chloride) Electrospun Fibers

Removal of Persistent Sulfamethoxazole and Carbamazepine from Water by Horseradish Peroxidase Encapsulated into Poly(Vinyl Chloride) Electrospun Fibers

A contemporary review of enzymatic applications in the remediation of emerging estrogenic compounds

Enhanced Wastewater Treatment by Immobilized Enzymes

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