Perspectives on the Incidence of Acanthamoeba Keratitis

Aiello, Francesco; Gallo Afflitto, Gabriele; Ceccarelli, Francesca; Turco, Maria Vittoria; Han, Yuyi; Amescua, Guillermo; Dart, John K.; Nucci, Carlo

doi:10.1016/j.ophtha.2024.08.003

Ophthalmology

2024

DOI: 10.1016/j.ophtha.2024.08.003

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Perspectives on the Incidence of Acanthamoeba Keratitis

Francesco Aiello,

Gabriele Gallo Afflitto,

Francesca Ceccarelli

et al.

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2024

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Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model

Rodríguez-Expósito,

Sifaoui,

Salazar-Villatoro

et al. 2024

Marine Drugs

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Acanthamoeba is a ubiquitous genus of amoebae that can trigger a severe and progressive ocular disease known as Acanthamoeba Keratitis (AK). Furthermore, current treatment protocols are based on the combination of different compounds that are not fully effective. Therefore, an urgent need to find new compounds to treat Acanthamoeba infections is clear. In the present study, we evaluated staurosporine as a potential treatment for Acanthamoeba keratitis using mouse cornea as an ex vivo model, and a comparative proteomic analysis was conducted to elucidate a mechanism of action. The obtained results indicate that staurosporine altered the conformation of actin and tubulin in treated trophozoites of A. castellanii. In addition, proteomic analysis of treated trophozoites revealed that this molecule induced overexpression and a downregulation of proteins related to key functions for Acanthamoeba infection pathways. Additionally, the ex vivo assay used validated this model for the study of the pathogenesis and therapies of AK. Finally, staurosporine eliminated the entire amoebic population and prevented the adhesion and infection of amoebae to the epithelium of treated mouse corneas.

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Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model

Rodríguez-Expósito,

Sifaoui,

Salazar-Villatoro

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

show abstract

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Perspectives on the Incidence of Acanthamoeba Keratitis

Cited by 1 publication

References 121 publications

Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model

Staurosporine as a Potential Treatment for Acanthamoeba Keratitis Using Mouse Cornea as an Ex Vivo Model

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