Chitosan and chitosan oligomers are receiving increasing attention due to their antimicrobial properties. In the present study, they were assayed as a preventive treatment against white-rot decay of Populus wood (very important in economic and environmental terms), caused by Trametes versicolor fungus. Their capacity to incorporate different chemical species into the polymer structure with a view to improving their anti-fungal activity was also assessed by mixing oligo-chitosan with propolis and silver nanoparticles. The minimum inhibitory concentration of medium-molecular weight chitosan (MMWC), chitosan oligomers (CO), propolis (P), nanosilver (nAg), and their binary and ternary composites against T. versicolor was determined in vitro. Although all products exhibited anti-fungal properties, composites showed an enhanced effect as compared to the individual products: 100% mycelial growth inhibition was attained for concentrations of 2.0 and 0.2 mg·mL −1 for the CO-P binary mixture, respectively; and 2 µg·mL −1 for nAg in the ternary mixture. Subsequently, MMWC, CO, CO-P and CO-P-nAg composites were tested on poplar wood blocks as surface protectors. Wood decay caused by the fungus was monitored by microscopy and vibrational spectroscopy, evidencing the limitations of the CO-based coatings in comparison with MMWC, which has a higher viscosity and better adhesion properties. The usage of MMWC holds promise for poplar wood protection, with potential industrial applications. Coatings 2018, 8, 415 2 of 15its main characteristics, such as its bioactivity, non-toxicity or biodegradability, its antimicrobial effect is especially relevant [4][5][6]. Its activity against different fungi, gram positive and gram negative bacteria, is ascribed to the positive charge of amino group (NH 3 + ), which interacts electrostatically with the surface of the cellular membrane, destabilizing it. As a consequence, the presence of chitosan inside the cell can lead to intracellular responses such as inactivation or blocking of enzymes activities, and of DNA transcription and translation [7,8].The antifungal activity of chitosan not only depends on the fungus species, but also on its molecular weight (MW), polymerization degree (PD) or deacetylation degree (DD). For instance, chito-oligosaccharides with low MW, PD and DD have been reported to be more effective on phytopathogenic fungi than chitosan with higher MW, PD or DD [9][10][11].Another known advantage from chitosan and its oligomers is associated with their sorption and chelating properties. The cationic nature of the polymer allows it to bind to different chemicals species, ranging from organic macromolecules to metal nanoparticles [12][13][14]. Natural oils or extracts of natural products can be incorporated into the chitosan matrix, for example, by cross-linking, producing solutions, films or beads [15][16][17]. In the particular case of propolis, its polyphenols have been reported to form hydrogen bonds and covalent bonds with the functional groups in chitosan [18]. This results...