Perturbations of gut microbiome and metabolome of pigs infected with Mycoplasma hyorhinis

Abstract

BackgroundMycoplasma hyorhinis is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a large impact on intestinal microecology. To study the effect of M. hyorhinis infection on gut microbial composition and metabolome profile, pigs were infected with M. hyorhinis. Metagenomic sequencing analysis was performed of fecal samples and a liquid chromatography/tandem mass spectrometry (LC–MS/MS) analysis of gut digesta was made.ResultsPigs infected with M. hyorhinis had enriched Sutterella and Mailhella, and depleted Dechloromonas, Succinatimonas, Campylobacter, Blastocystis, Treponema, and Megasphaera. The pigs infected with M. hyorhinis also had greater abundances of bacterium_0_1xD8_71, Ruminococcus_sp__CAG_353, Firmicutes_bacterium_CAG_194, Firmicutes_bacterium_CAG_534, bacterium_1xD42_87, and lower abundances of Chlamydia_suis, Megasphaera_elsdenii, Treponema_porcinum, Bacteroides_sp__CAG_1060, Faecalibacterium_prausnitzii. Metabolomic analysis revealed that some lipids and lipid‐like molecules increased in the small intestine, whereas most lipids and lipid‐like molecule metabolites decreased in the large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism, and thiamine metabolism.ConclusionThese findings demonstrate that infection with M. hyorhinis can alter the gut microbial composition and metabolite structure in pigs, which may further affect amino acid metabolism and lipid metabolism in the intestine. © 2023 Society of Chemical Industry.

Comparative analysis of intestinal microbiota composition between free-ranged captive yak populations in Nimu County

“…Disconcertingly, several genera ( Paenibacillaceae , Verrucomicrobiota , Akkermansia , Ruminococcaceae , Marvinbryantia , etc) which significantly increased in the NF group have been reported to be positively associated with metabolic diseases such as diabetes and obesity, including some SCFAs producers ( Palmas et al., 2021 ; Rondanelli et al., 2021 ; Deledda et al., 2022 ). The abundance of Lactobacillus and Olsenella relatively decreased in group NF, while Coriobacteriaceae _ UCG_ 002 relative increased, similar tendencies were also found in the gut microbiota of animals with intestinal diseases such as colitis and inflammatory bowel disease ( Zhang et al., 2020a ; Zhang et al., 2023 ). It can be speculated that although the body has made adaptations, the complex environment in pastoral areas still has certain adverse effects on the health status of grazing yaks.…”

Metagenomic analysis fecal microbiota of dysentery-like diarrhoea in a pig farm using next-generation sequencing

“…Metagenomic sequencing has demonstrated numerous advantages over conventional targeted detection technologies by detecting and characterizing multiple, unexpected, or novel pathogens, and even unculturable microbial species (11)(12)(13). Additionally, metagenomic sequencing analysis showed a higher level of phylotype resolution compared to 16S rRNA gene sequencing, thus providing more useful information to discover potential infection pathogens (14), such as Porcine circovirus type 2 (PCV2), Porcine sapelovirus (PSV), Porcine enterovirus, Escherichia coli, and Mycoplasma hyorhinis (15)(16)(17)(18)(19).…”

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“…Disconcertingly, several genera (Paenibacillaceae, Verrucomicrobiota, Akkermansia, Ruminococcaceae, Marvinbryantia, etc) which significantly increased in the NF group have been reported to be positively associated with metabolic diseases such as diabetes and obesity, including some SCFAs producers (Palmas et al, 2021;Rondanelli et al, 2021;Deledda et al, 2022). The abundance of Lactobacillus and Olsenella relatively decreased in group NF, while Coriobacteriaceae_ UCG_ 002 relative increased, similar tendencies were also found in the gut microbiota of animals with intestinal diseases such as colitis and inflammatory bowel disease (Zhang et al, 2020a;Zhang et al, 2023). It can be speculated that although the body has made adaptations, the complex environment in pastoral areas still has certain adverse effects on the health status of grazing yaks.…”