Pescoids and chimeras to probe early evo-devo in the fish<i>Astyanax mexicanus</i>

Torres-Paz, Jorge; Rétaux, Sylvie

doi:10.1101/2020.10.06.328500

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…This process of self-assembly, where cells aggregate and establish a pattern is not unique to the annual killifish and has also been observed experimentally after dissociating whole embryonic explants of Atlantic killifish [55], zebrafish [56,57] and cavefish [58] embryos. These explants have recently been termed Pescoids (figure 2 c ).…”

Section: Pattern Formation During Extensive Cell Rearrangementmentioning

confidence: 89%

The unappreciated generative role of cell movements in pattern formation

2022

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The mechanisms underpinning the formation of patterned cellular landscapes has been the subject of extensive study as a fundamental problem of developmental biology. In most cases, attention has been given to situations in which cell movements are negligible, allowing researchers to focus on the cell-extrinsic signalling mechanisms, and intrinsic gene regulatory interactions that lead to pattern emergence at the tissue level. However, in many scenarios during development, cells rapidly change their neighbour relationships in order to drive tissue morphogenesis, while also undergoing patterning. To draw attention to the ubiquity of this problem and propose methodologies that will accommodate morphogenesis into the study of pattern formation, we review the current approaches to studying pattern formation in both static and motile cellular environments. We then consider how the cell movements themselves may contribute to the generation of pattern, rather than hinder it, with both a species specific and evolutionary viewpoint.

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Section: Pattern Formation During Extensive Cell Rearrangementmentioning

confidence: 89%

The unappreciated generative role of cell movements in pattern formation

2022

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“…Lately, such explant system have also been derived from the Mexican tetra Astyanax mexicanus ( Torres-Paz and Retaux, 2021 ) and the Japanese rice fish or medaka Oryzias latipes ( Zilova et al, 2021 ). In the latter case, blastoderm cells were re-aggregated and exposed to factors which facilitate neural development to generate anterior structures recapitulating retinal differentiation as well as morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

Studying evolution of the primary body axis in vivo and in vitro

Anlaş

Trivedi

2021

eLife

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The metazoan body plan is established during early embryogenesis via collective cell rearrangements and evolutionarily conserved gene networks, as part of a process commonly referred to as gastrulation. While substantial progress has been achieved in terms of characterizing the embryonic development of several model organisms, underlying principles of many early patterning processes nevertheless remain enigmatic. Despite the diversity of (pre-)gastrulating embryo and adult body shapes across the animal kingdom, the body axes, which are arguably the most fundamental features, generally remain identical between phyla. Recently there has been a renewed appreciation of ex vivo and in vitro embryo-like systems to model early embryonic patterning events. Here, we briefly review key examples and propose that similarities in morphogenesis and associated gene expression dynamics may reveal an evolutionarily conserved developmental mode as well as provide further insights into the role of external or extraembryonic cues in shaping the early embryo. In summary, we argue that embryo-like systems can be employed to inform previously uncharted aspects of animal body plan evolution as well as associated patterning rules.

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Evolution of the regulation of developmental gene expression in blind Mexican cavefish

Leclercq

Torres-Paz

Policarpo

et al. 2022

Preprint

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Changes in gene expression regulation during development are considered the main drivers of morphological evolution and diversification. Here, we analysed the embryonic transcriptomes of surface-dwelling and blind cave-adapted morphs of the species Astyanax mexicanus and their reciprocal F1 hybrids at tailbud stage. Comparing gene expression in parents and allelic expression ratios in hybrids, we found that ~20% of the transcriptome is differentially expressed and that cis-regulatory changes are the main contributors to variations in early developmental gene expression in the two morphs. We provide a list of 108 cis-regulated genes that could contribute to cavefish developmental evolution, and further explore the regulatory mechanisms controlling the cellular and regional expression of rx3, a master eye gene. Using quantitative embryology approaches after fluorescent in situ hybridisation, cell transplantations and interference with signalling pathways, we show that rx3 cellular levels -controlling optic cell fates- are regulated in cis and in a cell-autonomous manner, whereas the size of rx3 domain -controlling eye size- depends on non-autonomous Wnt signalling. Altogether, we reveal how distinct mechanisms and regulatory modules can regulate developmental gene expression and shape developmental evolution, with negligible contribution of coding mutations.

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Pescoids and chimeras to probe early evo-devo in the fishAstyanax mexicanus

Cited by 3 publications

References 47 publications

The unappreciated generative role of cell movements in pattern formation

The unappreciated generative role of cell movements in pattern formation

Studying evolution of the primary body axis in vivo and in vitro

Evolution of the regulation of developmental gene expression in blind Mexican cavefish

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