Staphylococcus sp. are responsible for several clinical problems in fish farming and humans. This microorganism has a high risk associated with its presence in large numbers, as they have the ability to produce, under certain conditions, thermostable enterotoxins, which, when ingested, cause poisoning and various foodborne diseases. Present in aquaculture environments, Staphylococcus sp, have great potential to acquire antibiotic resistance, due to the use of such substances in the indiscriminate treatment and prophylaxis of bacterial diseases. The presence of food resistant Staphylococcus sp is a current problem in food production and marketing and is a potential danger to human health and a risk to the world fish industry. One hundred tilapia fillet samples were used for isolation of Staphylococccus sp., obtained from fishes located in northwestern Paraná state. It was submitted to antimicrobial susceptibility testing with ten antibiotics. Regarding the 100 samples analyzed, all showed resistance: Tetracycline 18%, Penicillin 31%, Enrofloxacin 14%, Erythromycin 12%, Vancomycin 15%, Gentamicin 14%, Oxacillin 20%, Ampicillin 28% and Cephalothin 11%. The emergence of resistant microorganisms in food is worrying as it can cause serious infections, hospitalizations, and death.