Pest survey card on Pseudomonas syringae pv.actinidiae

Vogelaar, Martijn; Schenk, Martijn; Delbianco, Alice; Graziosi, Ignazio; Vos, Sybren

doi:10.2903/sp.efsa.2020.en-1986

Cited by 3 publications

(3 citation statements)

References 59 publications

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Section: Detection and Diagnosis Of P Syringae With Conventional Methodsmentioning

confidence: 99%

“…Moreover, treatments are often ineffective once visible damage has already taken place. Farmers need to be able to identify a pathogen before the symptom becomes apparent to prevent irreparable harm to crops [96,97]. Microscopy examination is a technique that uses a microscope to magnify and observe small objects, including plant tissues and cells (Figure 3) [17][18][19].…”

Section: Detection and Diagnosis Of P Syringae With Conventional Methodsmentioning

confidence: 99%

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Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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Plant diseases caused by the pathogen Pseudomonas syringae are serious problems for various plant species worldwide. Accurate detection and diagnosis of P. syringae infections are critical for the effective management of these plant diseases. In this review, we summarize the current methods for the detection and diagnosis of P. syringae, including traditional techniques such as culture isolation and microscopy, and relatively newer techniques such as PCR and ELISA. It should be noted that each method has its advantages and disadvantages, and the choice of each method depends on the specific requirements, resources of each laboratory, and field settings. We also discuss the future trends in this field, such as the need for more sensitive and specific methods to detect the pathogens at low concentrations and the methods that can be used to diagnose P. syringae infections that are co-existing with other pathogens. Modern technologies such as genomics and proteomics could lead to the development of new methods of highly accurate detection and diagnosis based on the analysis of genetic and protein markers of the pathogens. Furthermore, using machine learning algorithms to analyze large data sets could yield new insights into the biology of P. syringae and novel diagnostic strategies. This review could enhance our understanding of P. syringae and help foster the development of more effective management techniques of the diseases caused by related pathogens.

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Section: Detection and Diagnosis Of P Syringae With Conventional Methodsmentioning

confidence: 99%

Section: Detection and Diagnosis Of P Syringae With Conventional Methodsmentioning

confidence: 99%

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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“…Pseudomonas syringae pv. actinidiae is one of over 60 P. syringae described pathovars and is subject to emergency measures that aim to prevent further spread of the pathogen in the European Union (EU) [1]. Bacterial canker of kiwifruit is an economically important disease caused by Pseudomonas syringae pv.…”

Section: Introductionmentioning

confidence: 99%

Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike

et al. 2021

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Currently, kiwifruit cultivation arouses great economic interest in the agricultural sector in several countries of the European Union due to high consumer demand and good results achieved in terms of production potential and fruit quality. One of the main bacterial species that cause yield losses in kiwifruit plants is Pseudomonas syringae. Diseases such as bacterial canker, caused by pathovar (pv.) actinidiae; floral bud necrosis caused by pv. syringae and leaf spots caused by pv. actinidifoliorum (Pfm) are clear examples. Between 2014 and 2017, in the main kiwifruit producing areas in the north and east of Spain, several surveys were carried out in search of these pathogens. Analyses realized from symptomatic and asymptomatic plants of Actinidiadeliciosa revealed the existence of new bacterial isolates close to Pfm. These new isolates werelow virulence pathogens similar to Pfm but belonging to a new group of P. syringae that affected the leaves of A. chinensis var. deliciosa. This study focused on the characterization and classification of these new isolates by a polyphasic approach in order to provide more information for understanding how the different populations of P.syringae affecting kiwifruit. They had the phenotypic characteristics of Pfm but by molecular approaches, they constituted a supported genetic lineage closely-related to Pfm independent of the five lineages described so far. This work revealed the great diversity found in P. syringae species affecting kiwifruit plants and supports the hypothesis that Pfm is a low virulence pathogen which is long established in Europe.

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Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens

Muthukumar,

Kamalakannan,

Johnson

et al. 2024

Plant Prot. Sci.

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Pest survey card on Pseudomonas syringae pv.actinidiae

Cited by 3 publications

References 59 publications

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike

Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens

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