Pesticide degrading natural multidrug resistance bacterial flora

Kirubakaran, Rangasamy; Athiappan, Murugan; Samykannu, Gopinath; Parray, Javid A.; ArulJothi, K.N.; Shameem, Nowsheen; Alqarawi, Abdulaziz A.; Abd_Allah, Elsayed Fathi

doi:10.1016/j.micpath.2017.12.013

Cited by 61 publications

(26 citation statements)

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“…Integrons are another MGE responsible for the emergence and spread of antibiotic resistance genes, including β-lactamases, aminoglycosides, and fluoroquinolones [38]. Transposons, like plasmids, have the potential to transfer horizontally or vertically among pathogens, driving the development of antibiotic resistance [39]. A genomic island (GI), usually with a size of 4.5–600 kb and generated by lateral gene transfer (LGT), is a large continuous genomic region.…”

Section: Resultsmentioning

confidence: 99%

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

et al. 2019

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Background Multidrug resistance is a growing global public health threat with far more serious consequences than generally anticipated. In this study, we investigated the antibiotic resistance and genomic traits of a clinical strain of Escherichia coli LCT-EC001. Results LCT-EC001 was resistant to 16 kinds of widely used antibiotics, including fourth-generation cephalosporins and carbapenems. In total, up to 68 determinants associated with antibiotic resistance were identified, including 8 beta-lactamase genes (notably producing ESBLs and KPCs), 31 multidrug efflux system genes, 6 outer membrane transport system genes, 4 aminoglycoside-modifying enzyme genes, 10 two-component regulatory system genes, and 9 other enzyme or transcriptional regulator genes, covering nearly all known drug-resistance mechanisms in E. coli . More than half of the resistance genes were located close to mobile genetic elements, such as plasmids, transposons, genomics islands, and insertion sequences. Phylogenetic analysis revealed that this strain may have evolved from E. coli K-12 but is a completely new MLST type. Conclusions Antibiotic resistance was extremely severe in E. coli LCT-EC001, mainly due to mobile genetic elements that allowed the gain of a large quantity of resistance genes. The antibiotic resistance genes of E. coli LCT-EC001 can probably be transferred to other bacteria. To the best of our knowledge, this is the first report of a strain of E. coli which has such a large amount of antibiotic resistance genes. Apart from providing an E. coli reference genome with an extremely high multidrug-resistant background for future analyses, this work also offers a strategy for investigating the complement and characteristics of genes contributing to drug resistance at the whole-genome level. Electronic supplementary material The online version of this article (10.1186/s13099-019-0298-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

et al. 2019

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“…Therefore, it may be concluded that the bacterial system itself has the inborn mechanism of carbofuran metabolism that is needed to be investigated. Advantageously, it has reported that pesticide resistance genes in microbes are always transferred as gene clusters [40], and they may also carry genes essential for antibiotic resistance.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

Ekram

Sarker

Rahi³

et al. 2020

J Basic Microbiol

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Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment-friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran-supplemented rice-field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse-phase high-performance liquid chromatography (RP-HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP-HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure).Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation. K E Y W O R D S antibiotic sensitivity, biodegradation, carbofuran, cytotoxicity, RP-HPLC How to cite this article: Ekram MA-E, Sarker I, Rahi MS, Rahman MA, Saha AK, Reza MA. Efficacy of soil-borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate.

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“…Heavy metal pollution in recent years has become a Global problem, as heavy metals unlike other pollutants, are non-biodegradable and bioaccumulate in the system ( Macdonald and Singh, 2014 ). In many microorganisms metal resistance is a common process which posses different mechanisms like extended delay stage, for adapting to heavy metal ions ( Rangasamy et al, 2018b ). In metal contaminated environments, heavy metal -resistant microbial species occur naturally and these metals generate selection pressures to enable this proliferation and survival ( Rahman and Singh, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

MALDI-TOF-MS and 16S rRNA characterization of lead tolerant metallophile bacteria isolated from saffron soils of Kashmir for their sequestration potential

Uqab

Nazir

Ganai

et al. 2020

Saudi Journal of Biological Sciences

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Toxic metal contamination in soils due industrialization is nowadays a concern to the scientists worldwide. The current study deals with the evaluation of response and tolerance by isolated metallophilic bacteria in different lead concentrations (100 ppm to 1000 ppm). By taking optical densities of the isolates, the minimum inhibitory concentration (MIC) of Pb 2+ were determined.16S rRNA and MALDI-TOF MS were used for the identification of the bacteria. Total of 37 isolates were observed, among them 04 ( Staphylococcus equorum, Staphylococcus warneri , Bacillus safensis and Bacillus thuringiensis ), isolated were detected having efficacy of Pb 2+ tolerance and sequestration at varying MIC. Furthermore, B. thuringiensis was observed to have highest (900 ppm) tolerance for lead and lowest (500 ppm) for Staphylococcus warneri. Moreover, the highest (65.3%) sequestration potential has been observed for B. thuringiensis and least (52.8%) for S. warneri. The tolerance and sequestration potential properties of these isolated species can be utilised to exterminate heavy metals and reduce their toxicity from the contaminated environment.

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Pesticide degrading natural multidrug resistance bacterial flora

Cited by 61 publications

References 50 publications

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

MALDI-TOF-MS and 16S rRNA characterization of lead tolerant metallophile bacteria isolated from saffron soils of Kashmir for their sequestration potential

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