The main objective of this review was to determine the main risks that agricultural workers are exposed to during pesticide application, which may have a harmful effect on their health and on public health. This systematic review was based on the PRISMA guidelines. A search for articles was conducted in the Medline/PubMed, ScienceDirect, and Web of Science databases. Fifteen articles were selected considering their assessment of agricultural workers’ knowledge, perceptions, attitudes, practices, and behaviours, identifying the main risks and risk factors for disease associated with the unsafe handling of pesticides. The main risk factors identified were age, education, pesticide safety training, farming experience, and contact with other farmers/intermediaries resulting in pesticide access. The most frequent risk behaviour was an application of pesticides without personal protective equipment (PPE), incorrect disposal of empty packaging and waste, and undervaluation of label information, as well as other unsafe practices. Multidisciplinary and more effective training must be delivered to enhance pesticide-safe usage. This will empower workers to adopt more conscious and safer behaviours while using pesticides.