This work aimed to evaluate changes in phytic phosphorus content as the response of three maize hybrids (ZP 457, ZP 5601, and ZP 606) to different pesticide treatments for European Corn Borer (ECB) attacks and, as well as the correlation between damage caused by ECB attack and Pphy content. In the experimental field, maize ears were sampled and dried at a moisture content of 14% for the determination of changes in phytic phosphorus content in the grain. The content of phytic phosphorus was determined using UV / VIS spectrophotometry. Observed results showed the highest content of phytic phosphorus for untreated control for all tested hybrids, compared to pesticide treatments. The content of Pphy in the tested maize kernel samples ranged from 2.12 to 3.40 mg g-1. A significant positive correlation between attacks of larvae and Pphy content could indicate activation of its antioxidative function as a response to damage caused by ECB attack.