Pesticide mediated oxidative stress induces genotoxicity and disrupts chromatin structure in fenugreek (Trigonella foenum - graecum L.) seedlings

Mahapatra, Kalyan; De, Sayanti; Banerjee, Samrat; Roy, Sujit

doi:10.1016/j.jhazmat.2019.02.056

Cited by 72 publications

(35 citation statements)

References 59 publications

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“…When studying the effect of pesticides of various concentrations on the growth of wheat roots, it turned out, that with the exception of metribuzin, all fungicides and herbicides signi cantly inhibited root growth, starting from a concentration of 0.10 µg / mL.It should be noted, that the analysis of literature data on the effect of pesticides on the cytogenetic rearrangements of higher plants, showed a huge (by orders of magnitude) spread of data on the studied concentrations from very high: 0.5-2.0 mg/mL [20] and 5.0-15.0 mg/ml [45] to very low: 0.002-0.4 µg/mL [46]. The authors of the works did not motivate the choice of concentrations, but noted the cytotoxic effects of pesticides, which were detected for both, high and low concentrations.…”

Section: Discussionmentioning

confidence: 94%

“…Using the Allium cepa test, the authors showed, that tricyclazole causes chromosomal abnormalities, but does not signi cantly affect the MI. In a research of Mahapatra et al [20] examined the cytotoxicity, genotoxicity, and prooxidant activity of herbicides at a concentration of 0.5 to 2.0 mg/mL in fenugreek culture. A high yield of cells with various chromosomal abnormalities was found under the in uence of all, including low, concentrations of herbicides.…”

Section: Discussionmentioning

confidence: 99%

“…Chromosome sticking for these pesticides was a major class of anomalies. The formation of disul de bonds can be the result of oxidative modi cations under conditions of oxidative stress, which fungicides and pesticides induce in cells [20]. However, direct chemical interactions of pesticides with chromatin, which lead to disruption of the molecular architectonics of chromosomes and their sticking, are not excluded [20,54].…”

Section: Discussionmentioning

confidence: 99%

“…Pesticides have selective and targeted activity against mycopathogens and crop weeds. However, the selectivity of pesticide activity is not absolute: the evolutionary conservatism of many molecular-cellular events determines the activity of pesticides with respect to non-target organisms [16,17,18,19,20,21,22]. In this regard, cytogenetic studies of pesticides with respect to cereal crops, which are the main source in the nutritional structure of the world's population, are very important.…”

Section: Introductionmentioning

confidence: 99%

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Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Menzyanova

Shishatskaya

Pyatina

et al. 2020

Preprint

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Using the example of 2-day-old Triticum aestivum seedlings, grown in hydroponic culture, the cytological effects of pesticides with various mechanisms of the damaging were studied: the herbicides metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl and the fungicides tebuconazole, epoxiconazole, and azoxystrobin. All pesticides showed a dose-dependent inhibitory effect on the development of the roots of Triticum aestivum, but did not affect the mitotic activity and did not reduce the mitotic index of root apex meristem cells. Herbicides and fungicides affected the duration of metaphase, anophase, prophase, and cytokinesis activity in the root apex meristem of the wheat, and these changes were specific for the six studied preparations. Under the influence of all pesticides, the number of abnormal cells increased significantly, to the greatest extent (10–12 times compared with control - distilled water) under the influence of herbicides tribenuron-methyl and phenoxaprop-ethyl and fungicides azoxystrobin and epoxiconazole; to a lesser extent - under the influence of metribuzin and tebuconazole (6–7 times). For all studied pesticides the manifestation of cyto- and genotoxicity, including both clastogenic and aneugenic effects, was revealed in relation to the non-target plant Triticum aestivum. Under the influence of pesticides, structural anomalies, caused by chromosome breakdown (bridges, fragments, micronuclei) and spindle damage (lagging chromosomes and their fragments, uneven chromosome separation) were detected in the cells of the Triticum aestivum root apex meristem.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Menzyanova

Shishatskaya

Pyatina

et al. 2020

Preprint

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“…It was suggested that the use of pesticides in agriculture could be better controlled. Mahapatra, De, Banerjee, and Roy (2019) reported cytotoxic and genotoxic abilities commonly used four pesticides in the environment. The pesticides investigated were tricyclazole, thiabendazole, plethora, and insecticides.…”

Section: Annual Literature Reviewmentioning

confidence: 99%

Pesticides and herbicides

Choudri

Charabi

Al‐Nasiri

et al. 2020

Water Environment Research

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This paper highlights a review of scientific papers published in the year 2019 regarding pesticides and herbicides. The scientific review presented in this paper includes the presence and occurrence of pesticides and herbicides in the environment. The entire review divided into different sections, which are grouped into four main sections. Each of these sections provides studies conducted on toxicology, ecological risk assessment, strategies of treatment, policies, modeling, and guidelines regarding pesticides and herbicides management.

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Physiology, Biochemistry, and Transcriptomics Jointly Reveal the Phytotoxicity Mechanism of Acetochlor on Pisum sativum L.

Ma,

Wei

et al. 2024

Enviro Toxic and Chemistry

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Acetochlor, as a commonly used pre‐emergent herbicide, can be toxic to crops and affect production if used improperly. However, the toxic mechanism of acetochlor on plants is not fully understood. The present study used a combination of transcriptomic analysis and physiological measurements to investigate the effects of short‐term (15‐day) exposure to different concentrations of acetochlor (1, 10, 20 mg/kg) on the morphology, physiology, and transcriptional levels of pea seedlings, aiming to elucidate the toxic response and resistance mechanisms in pea seedlings under herbicide stress. The results showed that the toxicity of acetochlor to pea seedlings was dose‐dependent, manifested as dwarfing and stem base browning with increasing concentrations, especially at 10 mg/kg and above. Analysis of the antioxidant system showed that from the 1 mg/kg treatment, malondialdehyde, superoxide dismutase, peroxidase, and glutathione peroxidase in peas increased with increasing concentrations of acetochlor, indicating oxidative damage. Analysis of the glutathione (GSH) metabolism system showed that under 10 mg/kg treatment, the GSH content of pea plants significantly increased, and GSH transferase activity and gene expression were significantly induced, indicating a detoxification response in plants. Transcriptomic analysis showed that after acetochlor treatment, differentially expressed genes in peas were significantly enriched in the phenylpropane metabolic pathway, and the levels of key metabolites (flavonoids and lignin) were increased. In addition, we found that acetochlor‐induced dwarfing of pea seedlings may be related to gibberellin signal transduction. Environ Toxicol Chem 2024;00:1–15. © 2024 SETAC

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Pesticide mediated oxidative stress induces genotoxicity and disrupts chromatin structure in fenugreek (Trigonella foenum - graecum L.) seedlings

Cited by 72 publications

References 59 publications

Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Cytological Effects of Herbicidal and Fungicidal Pesticides on Root Apex Meristem Cells of Triticum Aestivum

Pesticides and herbicides

Physiology, Biochemistry, and Transcriptomics Jointly Reveal the Phytotoxicity Mechanism of Acetochlor on Pisum sativum L.

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