Pesticide Resisdue Levels In Blood And Different Organs Of Pregnant Goat

Mohan, K. Murali

doi:10.9790/3013-31301921

Cited by 2 publications

(1 citation statement)

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“…However, as BeWo cells only represent part of the placental tissue and since placental tissue changes over pregnancy, it is difficult to translate this in vitro finding to the exact in vivo situation. Results on placental accumulation of the six triazoles in vivo are not available, but it has been elucidated that some pesticides were found to accumulate in placental tissue (Mohan and Singh 2013 ; Saxena et al 1981 ). Therefore, it might be of interest to pay attention to possible placental toxicity, for which the in vitro BeWo cell model might be useful for prioritization because as shown in the present study the model is able to provide information on the intracellular accumulation of compounds.…”

Section: Discussionmentioning

confidence: 99%

Extended evaluation on the ES-D3 cell differentiation assay combined with the BeWo transport model, to predict relative developmental toxicity of triazole compounds

Flick

Rietjens

et al. 2015

Arch Toxicol

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The mouse embryonic stem D3 (ES-D3) cell differentiation assay is based on the morphometric measurement of cardiomyocyte differentiation and is a promising tool to detect developmental toxicity of compounds. The BeWo transport model, consisting of BeWo b30 cells grown on transwell inserts and mimicking the placental barrier, is useful to determine relative placental transport velocities of compounds. We have previously demonstrated the usefulness of the ES-D3 cell differentiation assay in combination with the in vitro BeWo transport model to predict the relative in vivo developmental toxicity potencies of a set of reference azole compounds. To further evaluate this combined in vitro toxicokinetic and toxicodynamic approach, we combined ES-D3 cell differentiation data of six novel triazoles with relative transport rates obtained from the BeWo model and compared the obtained ranking to the developmental toxicity ranking as derived from in vivo data. The data show that the combined in vitro approach provided a correct prediction for in vivo developmental toxicity, whereas the ES-D3 cell differentiation assay as stand-alone did not. In conclusion, we have validated the combined in vitro approach for developmental toxicity, which we have previously developed with a set of reference azoles, for a set of six novel triazoles. We suggest that this combined model, which takes both toxicodynamic and toxicokinetic aspects into account, should be further validated for other chemical classes of developmental toxicants.

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Section: Discussionmentioning

confidence: 99%