Pesticides and Herbicides

Chelme-Ayala, Pamela; Li, Xiangfei; Wang, Yingnan; Pourrezaei, Parastoo; Smith, Daniel W.; El-Din, Mohamed Gamal

doi:10.2175/106143008x328743

Cited by 3 publications

(1 citation statement)

References 128 publications

(132 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The viability of HuWa TERT cells was clearly impacted by exposure to all selected environmental contaminants (Figure a), with EC 50 ranging from 6.0 (lindane) to 173.3 nM ( p,p’ -DDE); in order of increasing toxicity: lindane > chlorpyrifos > dieldrin > endosulfan > trifluralin > p,p’ -DDE. Each of our priority pesticides have been applied in lethal ecotoxicological studies with a variety of species. − Our observed DAPI EC50 values were consistent with the reported LC50 on several marine fish species for lindane (ranged 3.8 to 226 nM), chlorpyrifos (ranged 0.7 to 5 × 10 5 nM), dieldrin (ranged 2.6 to 207.4 nM), endosulfan (ranged 0.24 to 368.6 nM), , and trifluralin (ranged 25 to 2.2 × 10 6 nM) . To our knowledge, no in vitro cytotoxicity studies have reported on cetaceans for our candidate chemicals, with the exception of p,p’ -DDE.…”

Section: Resultsmentioning

confidence: 99%

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

Hosen

Sykes

Wood

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

Cetaceans are at elevated risk of accumulating persistent and lipophilic environmental contaminants due to their longevity and high proportion of body fat. Despite this, there is a paucity of taxa-specific chemical effect data, in part due to the ethical and logistical constraints in working with highly mobile aquatic species. Advances in cetacean cell culture have opened the door to the application of mainstream in vitro toxicological effect assessment approaches. Image-based cell profiling is a high-throughput, microscopy-based system commonly applied in drug development. It permits the analysis of the xenobiotic effect on multiple cell organelles simultaneously, hereby flagging its potential utility in the evaluation of chemical toxicodynamics. Here we exposed immortalized humpback whale skin fibroblasts (HuWaTERT) to six priority environmental contaminants known to accumulate in the Southern Ocean food web, in order to explore their subcellular organelle responses. Results revealed chemical-dependent modulation of mitochondrial texture, with the lowest observed effect concentrations for chlorpyrifos, dieldrin, trifluralin, and p,p’-dichlorodiphenyldichloroethane of 0.3, 4.1, 9.3, and 19.8 nM, respectively. By contrast, no significant changes were observed upon exposure to endosulfan and lindane. This study contributes the first fixed mitochondrial images of HuWaTERT and constitutes novel, taxa-specific chemical effect data in support of evidence-based conservation policy and management.

show abstract

Section: Resultsmentioning

confidence: 99%

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

Hosen

Sykes

Wood

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

A Macrocyclic Ligand as Receptor and Zn^II‐Complex Receptor for Anions in Water: Binding Properties and Crystal Structures

Ambrosi

Formica

Fusi

et al. 2011

Chemistry A European J

View full text Add to dashboard Cite

Binding properties of 24,29-dimethyl-6,7,15,16-tetraoxotetracyclo[19.5.5.0(5,8).0(14,17)]-1,4,9,13,18,21,24,29-octaazaenatriaconta-Δ(5,8),Δ(14,17)-diene ligand L towards Zn(II) and anions, such as the halide series and inorganic oxoanions (phosphate (Pi), sulfate, pyrophosphate (PPi), and others), were investigated in aqueous solution; in addition, the Zn(II)/L system was tested as a metal-ion-based receptor for the halide series. Ligand L is a cryptand receptor incorporating two squaramide functions in an over-structured chain that connects two opposite nitrogen atoms of the Me(2)[12]aneN(4) polyaza macrocyclic base. It binds Zn(II) to form mononuclear species in which the metal ion, coordinated by the Me(2)[12]aneN(4) moiety, lodges inside the three-dimensional cavity. Zn(II)-containing species are able to bind chloride and fluoride at the physiologically important pH value of 7.4; the anion is coordinated to the metal center but the squaramide units play the key role in stabilizing the anion through a hydrogen-bonding network; two crystal structures reported here clearly show this aspect. Free L is able to bind fluoride, chloride, bromide, sulfate, Pi, and PPi in aqueous solution. The halides are bound at acidic pH, whereas the oxoanions are bound in a wide range of pH values ranging from acidic to basic. The cryptand cavity, abundant in hydrogen-bonding sites at all pH values, allows excellent selectivity towards Pi to be achieved mainly at physiological pH 7.4. By joining amine and squaramide moieties and using this preorganized topology, it was possible, with preservation of the solubility of the receptor, to achieve a very wide pH range in which oxoanions can be bound. The good selectivity towards Pi allows its discrimination in a manner not easily obtainable with nonmetallic systems in aqueous environment.

show abstract

Physiological explanation of herbicide tolerance in Azolla pinnata R.Br.

Sarkar

Adak

2017

Annals of Agrarian Science

View full text Add to dashboard Cite

Pesticides and Herbicides

Cited by 3 publications

References 128 publications

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

A Macrocyclic Ligand as Receptor and Zn^II‐Complex Receptor for Anions in Water: Binding Properties and Crystal Structures

Physiological explanation of herbicide tolerance in Azolla pinnata R.Br.

Contact Info

Product

Resources

About

Pesticides and Herbicides

Cited by 3 publications

References 128 publications

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

A Macrocyclic Ligand as Receptor and ZnII‐Complex Receptor for Anions in Water: Binding Properties and Crystal Structures

Physiological explanation of herbicide tolerance in Azolla pinnata R.Br.

Contact Info

Product

Resources

About

A Macrocyclic Ligand as Receptor and Zn^II‐Complex Receptor for Anions in Water: Binding Properties and Crystal Structures