Pestivirus Species Potential Adventitious Contaminants of Biological Products

Giangaspero, Massimo

doi:10.4172/2329-9088.1000153

Cited by 16 publications

(11 citation statements)

References 52 publications

(59 reference statements)

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“…The presence of adventitious viruses in cell cultures is well-recognized as one of the main concerns in the manufacture of human and animal biological products. FBS is routinely used as a medium supplement and may harbor many viral agents, one of the most common being NCP-BVDV (27). The only previous study carried out to determine pestivirus contamination in Argentine FBS was published in 2000, which reported that 100 out of 200 (50%) FBS batches analyzed were BVDV-positive.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Pécora

Aguirreburualde

Ridpath³

et al. 2019

Front. Vet. Sci.

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Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. Therefore, 124 local samples of non-irradiated FBS originating from Argentina were surveyed for the presence of pestiviruses using RT-PCR. Amplicons from pestivirus positive samples were genotyped. Four samples were positive for HoBi virus-specific RT-PCR, while the BVDV-positive samples (n = 45) were classified as BVDV-1b (82.2%), BVDV-1a (13.3%), and BVDV-2 (4.5%). Virus isolation and serological profile assessment were performed for the four HoBi-positive FBS lots. These results confirm the circulation of HoBi-like virus in some regions of the Argentinean territory, highlighting the need to review the diagnostic techniques currently used in the clinical cases suspected of BVDV and in contamination control protocols for adventitious agents in cells and biotechnological products.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Pécora

Aguirreburualde

Ridpath³

et al. 2019

Front. Vet. Sci.

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“…Other peptides -AKIQDKEGIPPDQQR and LIFAGKQLEDGR -matched down-stream segments of the polypeptide chain of both BVDV and human ubiquitin confirming the existence and localization in this polypeptide region. BVDV is a common contaminant of bovine biological products (10,11). To test the contamination of brain extracts by BVDV from reagents we submitted the extraction solution containing sodium deoxycholate, porcine trypsin and inhibitors to the same analytical protocol used for protein extraction, except acetone precipitation to avoid protein losses.…”

Section: Main Textmentioning

confidence: 99%

Zika virus may not be alone: proteomics associates a bovine-like viral diarrhea virus to microcephaly

Nogueira

Velásquez

Melo³

et al. 2016

Preprint

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One Sentence Summary:Proteomics analysis lead us to suspect the presence of a Bovine-like viral diarrhea virus (BVDV-like) in the brain tissue of fetuses bearing microcephaly during the outbreak in Paraíba State, Brazil, 2015. AbstractNo direct experimental causal evidence confirms that the Zika virus is the sole etiological agent responsible for the development of brain malformations in human fetuses during pregnancy. We used a discovery-driven approach to analyze protein extracts of three Zika positive brains. Shotgun mass spectrometry (MS) proteomics did not identify any Zika protein in all samples. However, MS detected the presence of peptide(s) from the polyprotein of a Bovine-like viral diarrhea virus (BVDV-like) in Zika-positive brains. These results indicate that Zika virus may not be, per se, the only etiological agent responsible for microcephaly and suggests that discovery-driven approaches play an essential role in the screening of fluids or tissues for virus or other etiological agents. MAIN TEXTTo the Zika infection in Bahia, Brazil in 2015 (1) followed a dramatic outbreak of microcephaly in the Northeastern Brazilian States of Paraíba, Pernambuco and Bahia (2). In January 2016, Melo suggested a correlation between Zika infection and fetal microcephaly during a two case study of women living in Paraiba State who complained of symptoms of Zika virus infection during pregnancy; real time PCR confirmed the presence of Zika virus in children brain tissue and amniotic fluid (3). Other groups associated the presence of Zika virus to microcephaly (4) and reviewed the evidence for causality by several criteria (5). However, reports linking high incidence of human microcephaly to the presence of Zika virus do not disclose any experimental data that provide a direct link between microcephaly and Zika virus during fetal brain development in spite of reducing viability and growth of neurospheres and brain organoids (6). Here, we expand these findings using discovery-driven shotgun . CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/062596 doi: bioRxiv preprint first posted online Jul. 15, 2016; proteomics based on high resolution and accuracy mass spectrometry analysis to show the detection of a BVDV-like virus in tissue extracts of Zika positive brains. Microcephaly is associated with genetic disorders. Among others, chromosomal trisomy (Edward´s), Aicardi-Goutieres´ and Rett´s syndromes and X-chromosomal microcephaly and trans-placental infections by viruses or bacteria, including rubella, cytomegalovirus, herpes simplex, varicella zoster virus, HIV, arboviruses and maternal malnutrition or drug use and chemical intoxication of pregnant mothers (3-5, 7).Reports disclosed the incidence of Central Nervous System (CNS) malformations in fetuses and neonates after the Zika virus outbreak in French Polynesia; however, incidence of microcephaly associated with the outbreak was n...

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“…These cases have primarily involved bovine viral diarrhea virus in veterinary vaccines. [6] However, there have been a number of reported cases of viral contaminants detected in biological bulk harvest samples. The viral contaminants attributed to the use of FBS have included epizootic hemorrhagic disease virus, Cache Valley virus, reovirus type 2, and vesivirus 2117.…”

Section: Examples Of Adventitious Agents In Final Productmentioning

confidence: 99%

Gamma Irradiation of Animal Serum: General Regulatory Environment and Process Controls

Hanson¹,

Croonenborghs²,

Senescu³

et al. 2019

BioProcess J

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Pestivirus Species Potential Adventitious Contaminants of Biological Products

Cited by 16 publications

References 52 publications

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Molecular Characterization of Pestiviruses in Fetal Bovine Sera Originating From Argentina: Evidence of Circulation of HoBi-Like Viruses

Zika virus may not be alone: proteomics associates a bovine-like viral diarrhea virus to microcephaly

Gamma Irradiation of Animal Serum: General Regulatory Environment and Process Controls

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