PET Imaging of Crossed Cerebellar Diaschisis after Long-Term Cerebral Ischemia in Rats

Abstract

Crossed cerebellar diaschisis (CCD) is a decrease of regional blood flow and metabolism in the cerebellar hemisphere contralateral to the injured brain hemisphere as a common consequence of stroke. Despite CCD has been detected in patients with stroke using neuroimaging modalities, the evaluation of this phenomenon in rodent models of cerebral ischemia has been scarcely evaluated so far. Here, we report the in vivo evaluation of CCD after long-term cerebral ischemia in rats using positron emission tomography (PET) imaging with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG). Imaging studies were combined with neurological evaluation to assess functional recovery. In the ischemic territory, imaging studies showed a significant decrease in glucose metabolism followed by a progressive recovery later on. Conversely, the cerebellum showed a contralateral hypometabolism from days 7 to 14 after reperfusion. Neurological behavior showed major impaired outcome at day 1 after ischemia followed by a significant recovery of the sensorimotor function from days 7 to 28 after experimental stroke. Taken together, these results suggest that the degree of CCD after cerebral ischemia might be predictive of neurological recovery.

Longitudinal Synaptic Density PET with 11C‐UCB‐J 6 Months After Ischemic Stroke

“…Here, we show persistence of this diaschisis over months in accordance with studies reporting on this phenomenon several months after the insult 22,26 . However, this contrasts with other studies suggesting diaschisis to be a transient phenomenon as a reflection of temporary functional alterations 27,28 . Differences in included patients, study design, and functional imaging modalities utilized to study crossed cerebellar diaschisis could possibly explain this heterogeneity in study results.…”

“…22,26 However, this contrasts with other studies suggesting diaschisis to be a transient phenomenon as a reflection of temporary functional alterations. 27,28 Differences in included patients, study design, and functional imaging modalities utilized to study crossed cerebellar diaschisis could possibly explain this heterogeneity in study results. However, as SV2A PET can be regarded as structural correlate of synaptic function, our findings may suggest the functional disturbances to be accompanied (or partially explained) by structural alterations.…”

Transhemispheric Diaschisis after Unilateral Focal Cerebral Ischemia Reperfusion: A Longitudinal Voxel-Based Study by MRI

“…This can be done by using transcranial Doppler ultrasonography [67,68]. Although the importance of the degree of diaschisis in the prediction of neurological outcomes following cerebral ischemia being appreciated [69], our experiments were the first steps in acquiring quantitative hemispheric diaschisis data. The next step of this study would be to investigate the effect of age on diaschisis.…”

Inhibition of exosome release augments neuroinflammation following intracerebral hemorrhage

“…To assess cerebral glucose metabolism following ICH, the intensity of 18 F‐FDG signal in the ipsilateral side was normalized to contralateral side. Signal intensity in the PET images were analyzed using Amide image analysis software 25 …”

“…Signal intensity in the PET images were analyzed using Amide image analysis software. 25 2.9 | Luxol fast blue staining Luxol Fast Blue staining was used to assess the white matter (WM) damage. Brain sections incubated with Luxol Fast Blue staining solution at 60°C for 2 hours.…”